Detection of phosphatidyl serine on activated platelets' surface by flow cytometry in whole blood: A simpler test for the diagnosis of Scott syndrome

British Journal of Haematology

Volumn 171, Issue 2, 2015, Pages 290-292

  Halliez, Maxime ^a   Fouassier, Marc ^a   Robillard, Nelly ^a   Ternisien, Catherine ^a   Sigaud, Marianne ^a   Trossaert, Marc ^a   Bene, Marie Christine ^a  

^a NANTES UNIVERSITY HOSPITAL   (France)

Author keywords

Bleeding disorders; Flow cytometry; Phosphatidyl serine; Phospholipid flip flop; Scott syndrome

Indexed keywords

FIBRIN; FIBRINOGEN RECEPTOR; LIPOCORTIN 5; PHOSPHATIDYLSERINE; PROTHROMBIN; THROMBIN;

ANO6 GENE; BLOOD CLOTTING; BLOOD CLOTTING DISORDER; BLOOD CLOTTING TEST; DIAGNOSTIC TEST; DISEASE ASSOCIATION; FIBRIN FORMATION; FLOW CYTOMETRY; GENE; GENE MUTATION; HUMAN; LETTER; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; SCOTT SYNDROME; THROMBOCYTE ACTIVATION;

EID: 84942986693     PISSN: 00071048     EISSN: 13652141     Source Type: Journal    
DOI: 10.1111/bjh.13391     Document Type: Letter

References (9)

1. Bolton-Maggs, P.H., Chalmers, E.A., Collins, P.W., Harrison, P., Kitchen, S., Liesner, R.J., Minford, A., Mumford, A.D., Parapia, L.A., Perry, D.J., Watson, S.P., Wilde, J.T. & Williams, M.D. (2006) A review of inherited platelet disorders with guidelines for their management on behalf of the UKHCDO. British Journal of Haematology, 135, 603-633.
2. Dachary-Prigent, J., Freyssinet, J.M., Pasquet, J.M., Carron, J.C. & Nurden, A.T. (1993) Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: a flow cytometry study showing a role for free sulfhydryl groups. Blood, 81, 2554-2565.
3. Flores-Nascimento, M.C., Orsi, F.L., Yokoyama, A.P., Pereira, F.G., Lorand-Metze, I., De Paula, E.V., Castro, V. & Annichino-Bizzacchi, J.M. (2012) Diagnosis of Scott syndrome in patient with bleeding disorder of unknown cause. Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis, 23, 75-77.
4. Hayward, C.P. (2013) Improving blood disorder diagnosis: reflections on the challenges. International Journal of Laboratory Hematology, 35, 244-253.
5. Munnix, I.C., Harmsma, M., Giddings, J.C., Collins, P.W., Feijge, M.A., Comfurius, P., Heemskerk, J.W. & Bevers, E.M. (2003) Store-mediated calcium entry in the regulation of phosphatidylserine exposure in blood cells from Scott patients. Thrombosis and Haemostasis, 89, 687-695.
6. Suzuki, J., Umeda, M., Sims, P.J. & Nagata, S. (2010) Calcium-dependent phospholipid scrambling by TMEM16F. Nature, 468, 834-838.
7. Toti, F., Satta, N., Fressinaud, E., Meyer, D. & Freyssinet, J.M. (1996) Scott syndrome, characterized by impaired transmembrane migration of procoagulant phosphatidylserine and hemorrhagic complications, is an inherited disorder. Blood, 87, 1409-1415.
8. Weiss, H.J. (1994) Scott syndrome: a disorder of platelet coagulant activity. Seminars in Hematology, 31, 312-319.
9. Zwaal, R.F., Comfurius, P. & Bevers, E.M. (2005) Surface exposure of phosphatidylserine in pathological cells. Cellular and Molecular Life Sciences, 62, 971-988.