Paraformaldehyde fixation may lead to misinterpretation of the subcellular localization of plant high mobility group box proteins

PLoS ONE

Volumn 10, Issue 8, 2015, Pages

  Li, Man Wah ^a   Zhou, Liang ^a,b   Lam, Hon Ming ^a   Langowski, Jörg ^c  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b SOUTHERN MEDICAL UNIVERSITY   (China)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; HIGH MOBILITY GROUP B PROTEIN; NUCLEIC ACID; PARAFORMALDEHYDE; ARABIDOPSIS PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; FIXATIVE; FORMALDEHYDE; HIGH MOBILITY GROUP B1 PROTEIN; HMGB1 PROTEIN, ARABIDOPSIS; HYBRID PROTEIN; POLYMER;

ARABIDOPSIS; ARTICLE; CELL STRUCTURE; CELLULAR DISTRIBUTION; CHROMOSOME ANALYSIS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; IMMUNOFLUORESCENCE TEST; METAPHASE CHROMOSOME; NONHUMAN; PLANT CELL; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; TOBACCO; GENETICS; INTERPHASE; METABOLISM; METAPHASE; PLANT CHROMOSOME; PROCEDURES; TISSUE FIXATION;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHROMOSOMES, PLANT; FIXATIVES; FORMALDEHYDE; GREEN FLUORESCENT PROTEINS; HMGB1 PROTEIN; INTERPHASE; METAPHASE; POLYMERS; RECOMBINANT FUSION PROTEINS; TISSUE FIXATION; TOBACCO;

EID: 84942870573     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0135033     Document Type: Article

References (23)

1. Antosch M, Mortensen SA, Grasser KD (2012) Plant Proteins Containing High Mobility Group Box DNA-Binding Domains Modulate Different Nuclear Processes. Plant Physiol. 159: 875-883. doi: 10.1104/pp.112.198283 PMID: 22585776
2. Bianchi ME, Beltrame M, Paonessa G (1989) Specific Recognition of Cruciform DNA by Nuclear-Protein Hmg1. Science. 243: 1056-1059. PMID: 2922595
3. Park S, Lippard SJ (2011) Redox State-Dependent Interaction of HMGB1 and Cisplatin-Modified DNA. Biochemistry-Us. 50: 2567-2574.
4. Jaouen S, de Koning L, Gaillard C, Muselikova-Polanska E, Stros M, Strauss F (2005) Determinants of specific binding of HMGB1 protein to hemicatenated DNA loops. J Mol Biol. 353: 822-837. PMID: 16199053
5. Ugrinova I, Pashev IG, Pasheva EA (2009) Post-synthetic acetylation of HMGB1 protein modulates its interactions with supercoiled DNA. Mol Biol Rep. 36: 1399-1404. doi: 10.1007/s11033-008-9327-z PMID: 18670905
6. Grasser M, Lentz A, Lichota J, Merkle T, Grasser KD (2006) The Arabidopsis genome encodes structurally and functionally diverse HMGB-type proteins. J Mol Biol. 358: 654-664. PMID: 16563436
7. Launholt D, Merkle T, Houben A, Schulz A, Grasser KD (2006) Arabidopsis chromatin-associated HMGA and HMGB use different nucleartargeting signals and display highly dynamic localization within the nucleus. Plant Cell. 18: 2904-2918. PMID: 17114349
8. Pedersen DS, Merkle T, Marktl B, Lildballe DL, Antosch M, Bergmann T, et al. (2010) Nucleocytoplasmic Distribution of the Arabidopsis Chromatin-Associated HMGB2/3 and HMGB4 Proteins. Plant Physiol. 154: 1831-1841. doi: 10.1104/pp.110.163055 PMID: 20940346
9. Wu Q, Zhang WS, Pwee KH, Kumar PP (2003) Rice HMGB1 protein recognizes DNA structures and bends DNA efficiently. Arch Biochem Biophys. 411: 105-111. PMID: 12590928
10. Grasser M, Christensen JM, Peterhansel C, Grasser KD (2007) Basic and acidic regions flanking the HMG-box domain of maize HMGB1 and HMGB5 modulate the stimulatory effect on the DNA binding of transcription factor Dof2. Biochemistry-Us. 46: 6375-6382.
11. Lichota J, Ritt C, Grasser KD (2004) Ectopic expression of the maize chromosomal HMGB1 protein causes defects in root development of tobacco seedlings. Biochem Bioph Res Co. 318: 317-322.
12. Hu LS, Yang XY, Yuan DJ, Zeng FC, Zhang XL (2011) GhHmgB3 deficiency deregulates proliferation and differentiation of cells during somatic embryogenesis in cotton. Plant Biotechnol J. 9: 1038-1048. doi: 10.1111/j.1467-7652.2011.00617.x PMID: 21554528
13. Lildballe DL, Pedersen DS, Kalamajka R, Emmersen J, Houben A, Grasser KD (2008) The Expression Level of the Chromatin-Associated HMGB1 Protein Influences Growth, Stress Tolerance, and Transcriptome in Arabidopsis. J Mol Biol. 384: 9-21. doi: 10.1016/j.jmb.2008.09.014 PMID: 18822296
14. Friml J, Benkova E, Mayer U, Palme K, Muster G (2003) Automated whole mount localisation techniques for plant seedlings. Plant J. 34: 115-124. PMID: 12662314
15. Manzanero S, Arana P, Puertas MJ, Houben A (2000) The chromosomal distribution of phosphorylated histone H3 differs between plants and animals at meiosis. Chromosoma. 109: 308-317. PMID: 11007489
16. Pallier C, Scaffidi P, Chopineau-Proust S, Agresti A, Nordmann P, Bianchi ME, et al. (2003) Association of chromatin proteins high mobility group box (HMGB) 1 and HMGB2with mitotic chromosomes. Mol Biol Cell. 14: 3414-3426. PMID: 12925773
17. Brears T, Liu C, Knight TJ, Coruzzi GM (1993) Ectopic Overexpression of Asparagine Synthetase in Transgenic Tobacco. Plant Physiol. 103: 1285-1290. PMID: 12232020
18. Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco by-2 Cell-Line as the Hela-Cell in the Cell Biology of Higher-Plants. Int Rev Cytol. 132: 1-30.
19. Li WYF, Wong FL, Tsai SN, Phang TH, Shao GH, Lam HM (2006) Tonoplast-locatedGmCLC1 and GmNHX1 from soybean enhance NaCl tolerance in transgenic bright yellow (BY)-2 cells. Plant Cell Environ. 29: 1122-1137. PMID: 17080938
20. Wilson SM, Bacic A (2012) Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling of carbohydrate and protein epitopes. Nat Protoc. 7: 1716-1727. doi: 10.1038/nprot.2012.096 PMID: 22918389
21. McDonald KL, Auer M (2006) High-pressure freezing, cellular tomography, and structural cell biology. Biotechniques. 41: 137-143. PMID: 16925014
22. Kolotuev I (2014) Positional Correlative Anatomy of Invertebrate Model Organisms Increases Efficiency of TEM Data Production. Microsc Microanal. 20: 1392-1403. doi: 10.1017/S1431927614012999 PMID: 25180638
23. Xiong HQ, Zhou ZQ, Zhu MQ, Lv XH, Li AA, Li SW, et al. (2014) Chemical reactivation of quenched fluorescent protein molecules enables resin-embedded fluorescence microimaging. Nat Commun. 5.