Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis

Methods

Volumn 87, Issue , 2015, Pages 64-74

  Dragovic, R A ^a   Collett, G P ^a   Hole, P ^b   Ferguson, D J P ^a   Redman, C W ^a   Sargent, I L ^a   Tannetta, D S ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b MALVERN INSTRUMENTS LTD   (United Kingdom)

Author keywords

Exosomes; Extracellular vesicles; Flow cytometry; Fluorescence NTA; Microvesicles; Placenta perfusion

Indexed keywords

ALIX PROTEIN; ALKALINE PHOSPHATASE PLACENTA ISOENZYME; CARRIER PROTEIN; CD63 ANTIGEN; UNCLASSIFIED DRUG; ALKALINE PHOSPHATASE; BIOLOGICAL MARKER; CALCIUM BINDING PROTEIN; CD63 PROTEIN, HUMAN; CELL CYCLE PROTEIN; ESCRT PROTEIN; NANOPARTICLE; PDCD6IP PROTEIN, HUMAN;

ARTICLE; CELL ISOLATION; CENTRIFUGATION; CONTROLLED STUDY; ENZYME ANALYSIS; ERYTHROCYTE; EXOSOME; FILTRATION; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; FLUORESCENCE NANOPARTICLE TRACKING ANALYSIS; FRACTIONATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MEMBRANE MICROPARTICLE; MULTICOLOR FLOW CYTOMETRY; PLACENTA; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; STANDARDIZATION; SYNCYTIOTROPHOBLAST; WESTERN BLOTTING; CHEMISTRY; DEVICES; FEMALE; FLUORESCENCE; GENE EXPRESSION; GENETICS; METABOLISM; PERFUSION; PREGNANCY; PROCEDURES; TRANSMISSION ELECTRON MICROSCOPY; TROPHOBLAST; ULTRASTRUCTURE;

ALKALINE PHOSPHATASE; ANTIGENS, CD63; BIOMARKERS; BLOTTING, WESTERN; CALCIUM-BINDING PROTEINS; CELL CYCLE PROTEINS; CENTRIFUGATION; ENDOSOMAL SORTING COMPLEXES REQUIRED FOR TRANSPORT; EXOSOMES; FEMALE; FILTRATION; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; NANOPARTICLES; PERFUSION; PREGNANCY; TROPHOBLASTS;

EID: 84942567212     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2015.03.028     Document Type: Article

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