Detection of a new cfr-like gene, cfr(B), in Enterococcus faecium isolates recovered from human specimens in the United States as part of the SENTRY Antimicrobial Surveillance Program

Antimicrobial Agents and Chemotherapy

Volumn 59, Issue 10, 2015, Pages 6256-6261

  Deshpande, Lalitagauri M ^a   Ashcraft, Deborah S ^b   Kahn, Heather P ^b   Pankey, George ^b   Jones, Ronald N ^a   Farrell, David J ^a   Mendes, Rodrigo E ^a  

^a JMI LABORATORIES   (United States)

^b OCHSNER CLINIC FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; CHLORAMPHENICOL; CIPROFLOXACIN; COTRIMOXAZOLE; DALFOPRISTIN PLUS QUINUPRISTIN; DAPTOMYCIN; DORIPENEM; DOXYCYCLINE; ERYTHROMYCIN; FLUCONAZOLE; GENTAMICIN; LINEZOLID; METRONIDAZOLE; MICAFUNGIN; PIPERACILLIN PLUS TAZOBACTAM; RETAPAMULIN; RIBOSOME RNA; TEICOPLANIN; TETRACYCLINE; TIGECYCLINE; VANCOMYCIN; VIRGINIAMYCIN; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; BETA LACTAM; OXAZOLIDINONE DERIVATIVE; RECOMBINANT PROTEIN; RIBOSOME PROTEIN; RNA 23S; TRANSPOSON;

ABDOMINAL ABSCESS; ADULT; AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CASE REPORT; CFR LIKE GENE; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; GENE LOCATION; GENE SEQUENCE; HUMAN; MALE; MIDDLE AGED; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; ORCHITIS; PEPTOCLOSTRIDIUM DIFFICILE; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SARCOIDOSIS; SEPTIC SHOCK; SEQUENCE ANALYSIS; STAPHYLOCOCCUS AUREUS; TRANSPOSON; UNITED STATES; ALLELE; BACTERIAL CHROMOSOME; CHEMISTRY; CLASSIFICATION; DRUG EFFECTS; EPIDEMIOLOGICAL MONITORING; GENE DOSAGE; GENE EXPRESSION; GENETICS; GRAM-POSITIVE BACTERIAL INFECTIONS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR CLONING; MULTIDRUG RESISTANCE; PHYLOGENY;

ALLELES; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAMS; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ENTEROCOCCUS FAECIUM; EPIDEMIOLOGICAL MONITORING; GENE DOSAGE; GENE EXPRESSION; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MALE; MIDDLE AGED; OXAZOLIDINONES; PHYLOGENY; RECOMBINANT PROTEINS; RIBOSOMAL PROTEINS; RNA, RIBOSOMAL, 23S; STAPHYLOCOCCUS AUREUS; UNITED STATES;

EID: 84942279482     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.01473-15     Document Type: Article

References (41)

1. Flamm RK, Mendes RE, Hogan PA, Ross JE, Farrell DJ, Jones RN. 2015. In vitro activity of linezolid as assessed through the 2013 LEADER surveillance program. Diagn Microbiol Infect Dis 81:283-289. http://dx.doi.org/10.1016/j.diagmicrobio.2014.12.009.
2. Mendes RE, Deshpande LM, Jones RN. 2014. Linezolid update: stable in vitro activity following more than a decade of clinical use and summary of associated resistance mechanisms. Drug Resist Updat 17:1-12. http://dx.doi.org/10.1016/j.drup.2014.04.002.
3. Locke JB, Hilgers M, Shaw KJ. 2009. Mutations in ribosomal protein L3 are associated with oxazolidinone resistance in staphylococci of clinical origin. Antimicrob Agents Chemother 53:5275-5278. http://dx.doi.org/10.1128/AAC.01032-09.
4. Locke JB, Hilgers M, Shaw KJ. 2009. Novel ribosomal mutations in Staphylococcus aureus strains identified through selection with the oxazolidinones linezolid and torezolid (TR-700). Antimicrob Agents Chemother 53:5265-5274. http://dx.doi.org/10.1128/AAC.00871-09.
5. Floyd JL, Smith KP, Kumar SH, Floyd JT, Varela MF. 2010. LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus. Antimicrob Agents Chemother 54:5406-5412. http://dx.doi.org/10.1128/AAC.00580-10.
6. Wang Y, Lv Y, Cai J, Schwarz S, Cui L, Hu Z, Zhang R, Li J, Zhao Q, He T, Wang D, Wang Z, Shen Y, Li Y, Fessler AT, Wu C, Yu H, Deng X, Xia X, Shen J. 2015. A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin. J Antimicrob Chemother 70:2182-2190. http://dx.doi.org/10.1093/jac/dkv116.
7. Toh SM, Xiong L, Arias CA, Villegas MV, Lolans K, Quinn J, Mankin AS. 2007. Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. Mol Microbiol 64:1506-1514. http://dx.doi.org/10.1111/j.1365-2958.2007.05744.x.
8. Challand MR, Salvadori E, Driesener RC, Kay CW, Roach PL, Spencer J. 2013. Cysteine methylation controls radical generation in the Cfr radical AdoMet rRNA methyltransferase. PLoS One 8:e67979, http://dx.doi.org/10.1371/journal.pone.0067979.
9. Long KS, Poehlsgaard J, Kehrenberg C, Schwarz S, Vester B. 2006. The cfr rRNA methyltransferase confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Antimicrob Agents Chemother 50:2500-2505. http://dx.doi.org/10.1128/AAC.00131-06.
10. Kaminska KH, Purta E, Hansen LH, Bujnicki JM, Vester B, Long KS. 2010. Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria. Nucleic Acids Res 38:1652-1663. http://dx.doi.org/10.1093/nar/gkp1142.
11. Atkinson GC, Hansen LH, Tenson T, Rasmussen A, Kirpekar F, Vester B. 2013. Distinction between the Cfr methyltransferase conferring antibiotic resistance and the housekeeping RlmN methyltransferase. Antimicrob Agents Chemother 57:4019-4026. http://dx.doi.org/10.1128/AAC.00448-13.
12. Schwarz S, Werckenthin C, Kehrenberg C. 2000. Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri. Antimicrob Agents Chemother 44:2530-2533. http://dx.doi.org/10.1128/AAC.44.9.2530-2533.2000.
13. Mendes RE, Deshpande LM, Castanheira M, DiPersio J, Saubolle MA, Jones RN. 2008. First report of cfr-mediated resistance to linezolid in human staphylococcal clinical isolates recovered in the United States. Antimicrob Agents Chemother 52:2244-2246. http://dx.doi.org/10.1128/AAC.00231-08.
14. Bongiorno D, Campanile F, Mongelli G, Baldi MT, Provenzani R, Reali S, Lo Russo C, Santagati M, Stefani S. 2010. DNA methylase modifications and other linezolid resistance mutations in coagulase-negative staphylococci in Italy. J Antimicrob Chemother 65:2336-2340. http://dx.doi.org/10.1093/jac/dkq344.
15. Rajan V, Kumar VG, Gopal S. 2014. A cfr-positive clinical staphylococcal isolate from India with multiple mechanisms of linezolid-resistance. Indian J Med Res 139:463-467.
16. Gales AC, Deshpande LM, de Souza AG, Pignatari AC, Mendes RE. 2015. MSSA ST398/t034 carrying a plasmid-mediated cfr and erm(B) in Brazil. J Antimicrob Chemother 70:303-305. http://dx.doi.org/10.1093/jac/dku366.
17. Kehrenberg C, Schwarz S. 2006. Distribution of florfenicol resistance genes fexA and cfr among chloramphenicol-resistant Staphylococcus isolates. Antimicrob Agents Chemother 50:1156-1163. http://dx.doi.org/10.1128/AAC.50.4.1156-1163.2006.
18. Shen J, Wang Y, Schwarz S. 2013. Presence and dissemination of the multiresistance gene cfr in Gram-positive and Gram-negative bacteria. J Antimicrob Chemother 68:1697-1706. http://dx.doi.org/10.1093/jac/dkt092.
19. Liu Y, Wang Y, Schwarz S, Li Y, Shen Z, Zhang Q, Wu C, Shen J. 2013. Transferable multiresistance plasmids carrying cfr in Enterococcus spp. from swine and farm environment. Antimicrob Agents Chemother 57:42-48. http://dx.doi.org/10.1128/AAC.01605-12.
20. Diaz L, Kiratisin P, Mendes R, Panesso D, Singh KV, Arias CA. 2012. Transferable plasmid-mediated resistance to linezolid due to cfr in a human clinical isolate of Enterococcus faecalis. Antimicrob Agents Chemother 56:3917-3922. http://dx.doi.org/10.1128/AAC.00419-12.
21. Liu Y, Wang Y, Wu C, Shen Z, Schwarz S, Du XD, Dai L, Zhang W, Zhang Q, Shen J. 2012. First report of the multidrug resistance gene cfr in Enterococcus faecalis of animal origin. Antimicrob Agents Chemother 56: 1650-1654. http://dx.doi.org/10.1128/AAC.06091-11.
22. Patel SN, Memari N, Shahinas D, Toye B, Jamieson FB, Farrell DJ. 2013. Linezolid resistance in Enterococcus faecium isolated in Ontario, Canada. Diagn Microbiol Infect Dis 77:350-353. http://dx.doi.org/10.1016/j.diagmicrobio.2013.08.012.
23. Marin M, Martin A, Alcala L, Cercenado E, Iglesias C, Reigadas E, Bouza E. 2015. Clostridium difficile isolates with high linezolid MICs harbor the multiresistance gene cfr. Antimicrob Agents Chemother 59:586-589. http://dx.doi.org/10.1128/AAC.04082-14.
24. Schwarz S, Wang Y. 2015. Nomenclature and functionality of the so-called cfr gene from Clostridium difficile. Antimicrob Agents Chemother 59:2476-2477. http://dx.doi.org/10.1128/AAC.04893-14.
25. Mendes RE, Flamm RK, Hogan PA, Ross JE, Jones RN. 2014. Summary of linezolid activity and resistance mechanisms detected during the 2012 LEADER surveillance program for the United States. Antimicrob Agents Chemother 58:1243-1247. http://dx.doi.org/10.1128/AAC.02112-13.
26. Mendes RE, Deshpande LM, Bonilla HF, Schwarz S, Huband MD, Jones RN, Quinn JP. 2013. Dissemination of a pSCFS3-like cfr-carrying plasmid in Staphylococcus aureus and Staphylococcus epidermidis clinical isolates recovered from hospitals in Ohio. Antimicrob Agents Chemother 57:2923-2928. http://dx.doi.org/10.1128/AAC.00071-13.
27. Kahn HP, Deshpande LM, Mendes RE, Ashcraft DS, Pankey G. 2015. Abstr 115th Gen Meet Am Soc Microbiol, abstr 103.
28. Clinical and Laboratory Standards Institute. 2015. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard - 10th ed. CLSI document M07-A10. Clinical and Laboratory Standards Institute, Wayne, PA.
29. Clinical and Laboratory Standards Institute. 2015. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. CLSI M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA.
30. Miller K, Dunsmore CJ, Fishwick CW, Chopra I. 2008. Linezolid and tiamulin cross-resistance in Staphylococcus aureus mediated by point mutations in the peptidyl transferase center. Antimicrob Agents Chemother 52:1737-1742. http://dx.doi.org/10.1128/AAC.01015-07.
31. Hansen LH, Planellas MH, Long KS, Vester B. 2012. The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene. Antimicrob Agents Chemother 56:3563-3567. http://dx.doi.org/10.1128/AAC.00673-12.
32. Dingle KE, Elliott B, Robinson E, Griffiths D, Eyre DW, Stoesser N, Vaughan A, Golubchik T, Fawley WN, Wilcox MH, Peto TE, Walker AS, Riley TV, Crook DW, Didelot X. 2014. Evolutionary history of the Clostridium difficile pathogenicity locus. Genome Biol Evol 6:36-52. http://dx.doi.org/10.1093/gbe/evt204.
33. Liu Y, Wang Y, Schwarz S, Wang S, Chen L, Wu C, Shen J. 2014. Investigation of a multiresistance gene cfr that fails to mediate resistance to phenicols and oxazolidinones in Enterococcus faecalis. J Antimicrob Chemother 69:892-898. http://dx.doi.org/10.1093/jac/dkt459.
34. Lobritz M, Hutton-Thomas R, Marshall S, Rice LB. 2003. Recombination proficiency influences frequency and locus of mutational resistance to linezolid in Enterococcus faecalis. Antimicrob Agents Chemother 47:3318-3320. http://dx.doi.org/10.1128/AAC.47.10.3318-3320.2003.
35. Mendes RE, Hogan PA, Streit JM, Jones RN, Flamm RK. 2015. Update on the linezolid in vitro activity through the Zyvox Annual Appraisal of Potency and Spectrum program, 2013. Antimicrob Agents Chemother 59:2454-2457. http://dx.doi.org/10.1128/AAC.04784-14.
36. Mendes RE, Hogan PA, Streit JM, Jones RN, Flamm RK. 2014. Zyvox Annual Appraisal of Potency and Spectrum (ZAAPS) program: report of linezolid activity over nine years (2004-12). J Antimicrob Chemother 69: 1582-1588. http://dx.doi.org/10.1093/jac/dkt541.
37. McBride SM, Fischetti VA, Leblanc DJ, Moellering RC, Jr, Gilmore MS. 2007. Genetic diversity among Enterococcus faecalis. PLoS One 2:e582. http://dx.doi.org/10.1371/journal.pone.0000582.
38. Arias CA, Panesso D, McGrath DM, Qin X, Mojica MF, Miller C, Diaz L, Tran TT, Rincon S, Barbu EM, Reyes J, Roh JH, Lobos E, Sodergren E, Pasqualini R, Arap W, Quinn JP, Shamoo Y, Murray BE, Weinstock GM. 2011. Genetic basis for in vivo daptomycin resistance in enterococci. N Engl J Med 365:892-900. http://dx.doi.org/10.1056/NEJMoa1011138.
39. Kehrenberg C, Ojo KK, Schwarz S. 2004. Nucleotide sequence and organization of the multiresistance plasmid pSCFS1 from Staphylococcus sciuri. J Antimicrob Chemother 54:936-939. http://dx.doi.org/10.1093/jac/dkh457.
40. Nallapareddy SR, Singh KV, Murray BE. 2006. Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains. Appl Environ Microbiol 72:334-345. http://dx.doi.org/10.1128/AEM.72.1.334-345.2006.
41. Su YC, Wong AC. 1995. Identification and purification of a new staphylococcal enterotoxin, H. Appl Environ Microbiol 61:1438-1443.