Back to basics - The influence of DNA extraction and primer choice on phylogenetic analysis of activated sludge communities

PLoS ONE

Volumn 10, Issue 7, 2015, Pages

  Albertsen, Mads ^a   Karst, Søren M ^a   Ziegler, Anja S ^a   Kirkegaard, Rasmus H ^a   Nielsen, Per H ^a  

^a AALBORG UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; PRIMER DNA; RNA 16S; SEWAGE;

ACTIVATED SLUDGE; AMPLICON; ARTICLE; BACTERIAL CELL WALL; COMPUTER MODEL; CONTROLLED STUDY; DNA EXTRACTION; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC ANALYSIS; GRAM POSITIVE BACTERIUM; METAGENOMICS; MICROBIAL COMMUNITY; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; CLASSIFICATION; DNA SEQUENCE; GENETICS; GRAM NEGATIVE BACTERIUM; ISOLATION AND PURIFICATION; MICROBIAL CONSORTIUM; MICROBIOLOGY; PROCEDURES; SEWAGE; SOLID PHASE EXTRACTION;

DNA PRIMERS; DNA, BACTERIAL; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; IN SITU HYBRIDIZATION, FLUORESCENCE; METAGENOMICS; MICROBIAL CONSORTIA; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SEWAGE; SOLID PHASE EXTRACTION;

EID: 84941331779     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0132783     Document Type: Article

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