High-throughput method of whole-brain sectioning, using the tape-transfer technique

PLoS ONE

Volumn 10, Issue 7, 2015, Pages

  Pinskiy, Vadim ^a,b   Jones, Jamie ^a   Tolpygo, Alexander S ^a   Franciotti, Neil ^a   Weber, Kevin ^a   Mitra, Partha P ^a  

^a Cold Spring Harbor Laboratory ^*  (United States)

^b Stony Brook University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CONTROLLED STUDY; EQUIPMENT DESIGN; FLUORESCENCE; FROZEN SECTION; HIGH THROUGHPUT SCREENING; HISTOCHEMISTRY; INTERMETHOD COMPARISON; MALE; MEDICAL PROCEDURES; MOUSE; MYELIN STAINING; NISSL STAINING; NONHUMAN; PROCESS DESIGN; QUALITY CONTROL; STAINING; TAPE TRANSFER TECHNIQUE; ANIMAL; BRAIN; C57BL MOUSE; CHEMISTRY; DEVICES; FLUORESCENCE MICROSCOPY; HAPLORHINI; LONG EVANS RAT; MICROTOMY; MYELIN SHEATH; NISSL SUBSTANCE; PROCEDURES; RAT; ULTRAMICROTOMY; ULTRASTRUCTURE;

DIAMINOBENZIDINE;

3,3'-DIAMINOBENZIDINE; ANIMALS; BRAIN; CRYOULTRAMICROTOMY; HAPLORHINI; HIGH-THROUGHPUT SCREENING ASSAYS; MALE; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE; MICROTOMY; MYELIN SHEATH; NISSL BODIES; RATS; RATS, LONG-EVANS; STAINING AND LABELING;

EID: 84941308382     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0102363     Document Type: Article

References (12)

1. CryoJane-Operating-Manual. Leica Microsystems Inc.: St. Louis, MO.
2. Pinskiy V, Tolpygo AS, Jones J, Weber K, Franciotti N, Mitra PP. A low-cost technique to cryo-protect and freeze rodent brains, precisely aligned to stereotaxic coordinates for whole-brain cryosectioning. J Neurosci Methods, 2013; 218: 206-13. doi: 10.1016/j.jneumeth.2013.03.004 PMID: 23541995
3. Peters SR. A Practical Guide to Frozen Section Technique. Springer, 2009.
4. Reiner A, Veenman CL, Medina L, Jiao Y, Del Mar N, Honig MG. Pathway tracing using biotinylated dextran amines. J Neurosci Methods, 2000; 103: 23-37. PMID: 11074093
5. Akins MR, Greer CA. Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion. J Comp Neurol, 2006; 499: 979-89. PMID: 17072833
6. Laux A, Muller AH, Miehe M, Dirrig-Grosch S, Deloulme JC, Delalande F, Stuber D, Sage D, Van Dorsselaer A, Poisbeau P, Aunis D, Goumon Y. Mapping of endogenous morphine-like compounds in the adult mouse brain: Evidence of their localization in astrocytes and GABAergic cells. J Comp Neurol, 2011; 519: 2390-416. doi: 10.1002/cne.22633 PMID: 21456021
7. Gallyas F. Silver staining of myelin by means of physical development. Neurological research, 1979; 1: 203-9. PMID: 95356
8. Microm-HM550. Cryostat MICROTOM (OMVPD) Operating Manual. Thermo Fisher Scientific: Kalamazoo, MI, 2007.
9. Jacquet R, Hillyer J, Landis WJ. Analysis of connective tissues by laser capture microdissection and reverse transcriptase-polymerase chain reaction. Analytical Biochemistry, 2005; 337: 22-34. PMID: 15649372
10. Jiang X, Kalajzic Z, Maye P, Braut A, Bellizzi J, Mina M, Rowe DW. Histological analysis of GFP expression in murine bone. J Histochem Cytochem, 2005; 53: 593-602. PMID: 15872052
11. Salie R, Li H, Jiang X, Rowe D, Kalajzic I, Susa M. A Rapid, Nonradioactive In Situ Hybridization Technique for Use on Cryosectioned Adult Mouse Bone. Calcified Tissue International, 2008; 83: 212-21.
12. Swanson LW. Brain Maps III: Structure of the Rat Brain. Academic, 2003. doi: 10.1007/s00223-008- 9154-1 PMID: 18762852