A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides

Nature Biotechnology

Volumn 33, Issue 7, 2015, Pages 743-749

  Chick, Joel M ^a   Kolippakkam, Deepak ^a   Nusinow, David P ^a   Zhai, Bo ^a   Rad, Ramin ^a   Huttlin, Edward L ^a   Gygi, Steven P ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; CHEMICAL MODIFICATION; INFORMATION RETRIEVAL; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PEPTIDES; SEARCH ENGINES;

DATA SET; DATABASE SEARCHES; HEK293 CELLS; HIGH CONFIDENCE; SEQUEST; SHOTGUN PROTEOMICS; TANDEM MASS SPECTROMETRY;

PROTEINS;

AMINO ACID; PEPTIDE; PROTEOME; PROTEIN;

ANIMAL TISSUE; ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; DATA ANALYSIS SOFTWARE; DATA BASE; ELECTROSPRAY MASS SPECTROMETRY; GLYCOSYLATION; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHYLATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEOMICS; SHOTGUN PROTEOMICS; STOICHIOMETRY; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; CHEMISTRY; HEK293 CELL LINE; MOLECULAR GENETICS; PEPTIDE MAPPING; PROCEDURES; PROTEIN DATABASE;

AMINO ACID SEQUENCE; DATABASES, PROTEIN; HEK293 CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PEPTIDES; PROTEINS; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 84941266080     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3267     Document Type: Article

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