POMGNT1 is glycosylated by mucin-type O-glycans

Biological and Pharmaceutical Bulletin

Volumn 38, Issue 9, 2015, Pages 1389-1394

  Xin, Xin ^a,b   Akasaka Manya, Keiko ^a   Manya, Hiroshi ^a   Furukawa, Jun Ichi ^c   Kuwahara, Naoyuki ^d   Okada, Kazue ^c   Tsumoto, Hiroki ^a   Higashi, Nobuaki ^b   Kato, Ryuichi ^d   Shinohara, Yasuro ^c   Irimura, Tatsuro ^e   Endo, Tamao ^a  

^a Tokyo Metropolitan Institute of Gerontology   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

^d INSTITUTE OF MATERIALS STRUCTURE SCIENCE   (Japan)

^e JUNTENDO UNIVERSITY   (Japan)

Author keywords

Glycan structure; Mucin type O glycan; O glycosylation; Protein O linked mannose 1,2 N acetylglucosaminyltransferase 1

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; GLYCAN; GLYCOSYLTRANSFERASE; LECTIN; MUCIN TYPE O GLYCAN; N ACETYLGLUCOSAMINE; PROTEIN O LINKED MANNOSE BETA1,2 N ACETYLGLUCOSAMINYLTRANSFERASE 1; PYRAZOLONE; RECOMBINANT ENZYME; SIALIDASE; UNCLASSIFIED DRUG; N ACETYLGLUCOSAMINYLTRANSFERASE; PLANT LECTIN; POLYSACCHARIDE; PROTEIN O-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE; RECOMBINANT PROTEIN; RIBOSOME INACTIVATING PROTEIN; SAMBUCUS NIGRA LECTINS;

ARTICLE; CARBOHYDRATE ANALYSIS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME MODIFICATION; HEK293 CELL LINE; HYDROPHOBICITY; MASS SPECTROMETRY; STRUCTURE ANALYSIS; GLYCOSYLATION; HUMAN; METABOLISM;

GLYCOSYLATION; HEK293 CELLS; HUMANS; N-ACETYLGLUCOSAMINYLTRANSFERASES; PLANT LECTINS; POLYSACCHARIDES; RECOMBINANT PROTEINS; RIBOSOME INACTIVATING PROTEINS;

EID: 84941209205     PISSN: 09186158     EISSN: 13475215     Source Type: Journal    
DOI: 10.1248/bpb.b15-00415     Document Type: Article

References (20)

1. Yoshida A, Kobayashi K, Manya H, Taniguchi K, Kano H, Mizuno M, Inazu T, Mitsuhashi H, Takahashi S, Takeuchi M, Herrmann R, Straub V, Talim B, Voit T, Topaloglu H, Toda T, Endo T. Muscular dystrophy and neuronal migration disorder caused by mutations in a glycosyltransferase, POMGnT1. Dev. Cell, 1, 717-724 (2001).
2. Endo T. Glycobiology of alpha-dystroglycan and muscular dystrophy. J. Biochem., 157, 1-12 (2015).
3. Xiong H, Kobayashi K, Tachikawa M, Manya H, Takeda S, Chiyonobu T, Fujikake N, Wang F, Nishimoto A, Morris GE, Nagai Y, Kanagawa M, Endo T, Toda T. Molecular interaction between fukutin and POMGnT1 in the glycosylation pathway of alpha-dystroglycan. Biochem. Biophys. Res. Commun., 350, 935-941 (2006).
4. Akasaka-Manya K, Manya H, Kobayashi K, Toda T, Endo T. Structure-function analysis of human protein O-linked mannose beta1, 2-N-acetylglucosaminyltransferase 1, POMGnT1. Biochem. Biophys. Res. Commun., 320, 39-44 (2004).
5. Manya H, Sakai K, Kobayashi K, Taniguchi K, Kawakita M, Toda T, Endo T. Loss-of-function of an N-acetylglucosaminyltransferase, POMGnT1, in muscle-eye-brain disease. Biochem. Biophys. Res. Commun., 306, 93-97 (2003).
6. "Essentials of Glycobiology." (Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, Hart GW, Etzler ME eds.) Cold Spring Harbor, NY (2009).
7. Topaz O, Shurman DL, Bergman R, Indelman M, Ratajczak P, Mizrachi M, Khamaysi Z, Behar D, Petronius D, Friedman V, Zelikovic I, Raimer S, Metzker A, Richard G, Sprecher E. Mutations in GALNT3, encoding a protein involved in O-linked glycosylation, cause familial tumoral calcinosis. Nat. Genet., 36, 579-581 (2004).
8. Ichikawa S, Sorenson AH, Austin AM, Mackenzie DS, Fritz TA, Moh A, Hui SL, Econs MJ. Ablation of the Galnt3 gene leads to low-circulating intact fibroblast growth factor 23 (Fgf23) concentrations and hyperphosphatemia despite increased Fgf23 expression. Endocrinology, 150, 2543-2550 (2009).
9. Schjoldager KT, Vester-Christensen MB, Bennett EP, Levery SB, Schwientek T, Yin W, Blixt O, Clausen H. O-Glycosylation modulates proprotein convertase activation of angiopoietin-like protein 3: possible role of polypeptide GalNAc-transferase-2 in regulation of concentrations of plasma lipids. J. Biol. Chem., 285, 36293-36303 (2010).
10. Holleboom AG, Karlsson H, Lin RS, Beres TM, Sierts JA, Herman DS, Stroes ES, Aerts JM, Kastelein JJ, Motazacker MM, Dallinga-Thie GM, Levels JH, Zwinderman AH, Seidman JG, Seidman CE, Ljunggren S, Lefeber DJ, Morava E, Wevers RA, Fritz TA, Tabak LA, Lindahl M, Hovingh GK, Kuivenhoven JA. Heterozygosity for a loss-of-function mutation in GALNT2 improves plasma triglyceride clearance in man. Cell Metab., 14, 811-818 (2011).
11. Park JH, Katagiri T, Chung S, Kijima K, Nakamura Y. Polypeptide N-acetylgalactosaminyltransferase 6 disrupts mammary acinar morphogenesis through O-glycosylation of fibronectin. Neoplasia, 13, 320-326, IN9-IN10 (2011).
12. Wasser CR, Masiulis I, Durakoglugil MS, Lane-Donovan C, Xian X, Beffert U, Agarwala A, Hammer RE, Herz J. Differential splicing and glycosylation of Apoer2 alters synaptic plasticity and fear learning. Sci. Signal., 7, ra113 (2014).
13. Furukawa J, Fujitani N, Araki K, Takegawa Y, Kodama K, Shinohara Y. A versatile method for analysis of serine/threonine posttranslational modifications by beta-elimination in the presence of pyrazolone analogues. Anal. Chem., 83, 9060-9067 (2011).
14. Takahashi S, Sasaki T, Manya H, Chiba Y, Yoshida A, Mizuno M, Ishida H, Ito F, Inazu T, Kotani N, Takasaki S, Takeuchi M, Endo T. A new beta-1, 2-N-acetylglucosaminyltransferase that may play a role in the biosynthesis of mammalian O-mannosyl glycans. Glycobiology, 11, 37-45 (2001).
15. Pereira MEA, Kabat EA, Lotan R, Sharon N. Immunochemical studies on the specificity of the peanut (Arachis hypogaea) agglutinin. Carbohydr. Res., 51, 107-118 (1976).
16. Wyss DF, Choi JS, Li J, Knoppers MH, Willis KJ, Arulanandam AR, Smolyar A, Reinherz EL, Wagner G. Conformation and function of the N-linked glycan in the adhesion domain of human CD2. Science, 269, 1273-1278 (1995).
17. Mer G, Hietter H, Lefevre JF. Stabilization of proteins by glycosylation examined by NMR analysis of a fucosylated proteinase inhibitor. Nat. Struct. Biol., 3, 45-53 (1996).
18. Stryer L. The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites. J. Mol. Biol., 13, 482-495 (1965).
19. Hawe A, Sutter M, Jiskoot W. Extrinsic fluorescent dyes as tools for protein characterization. Pharm. Res., 25, 1487-1499 (2008).
20. Matsuura H, Greene T, Hakomori S. An alpha-N-acetylgalactosaminylation at the threonine residue of a defined peptide sequence creates the oncofetal peptide epitope in human fibronectin. J. Biol. Chem., 264, 10472-10476 (1989).