Isolation and cryopreservation of neonatal rat cardiomyocytes

Journal of Visualized Experiments

Volumn 2015, Issue 98, 2015, Pages

  Vandergriff, Adam C ^a,b   Hensley, Michael Taylor ^a,b   Cheng, Ke ^a,b,c  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b USA and North Carolina State University   (United States)

^c Department of Philosophy   (United States)

Author keywords

Cardiac research; Cardiomyocytes; Cell culture; Cell based assay; Cryopreservation; Developmental biology; In vitro assay; Issue 98

Indexed keywords

ACTININ; CRYOPROTECTIVE AGENT; DIMETHYL SULFOXIDE;

ANIMAL; CARDIAC MUSCLE CELL; CELL CULTURE TECHNIQUE; CELL SEPARATION; CELL SURVIVAL; CHEMISTRY; CRYOPRESERVATION; CYTOLOGY; FREEZING; METABOLISM; NEWBORN; PHYSIOLOGY; PROCEDURES; RAT; SARCOMERE;

ACTININ; ANIMALS; ANIMALS, NEWBORN; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELL SURVIVAL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DIMETHYL SULFOXIDE; FREEZING; MYOCYTES, CARDIAC; RATS; SARCOMERES;

EID: 84940735270     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52726     Document Type: Article

References (19)

1. Louch, W. E., Sheehan, K. Methods in cardiomyocyte isolation, culture, and gene transfer. Journal of Molecular and Cellular Cardiology. 51, (3), 288-298 (2011).
2. Miragoli, M., Salvarani, N., Rohr, S. Myofibroblasts Induce Ectopic Activity in Cardiac Tissue. Circulation Research. 101, 755-758 (2007).
3. Simpson, P., Savion, S. Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Crossstriations, ultrastructure, and chronotropic response to isoproterenol. Circulation Research. 50, (1), 101-116 (1982).
4. Simpson, P., McGrath, A, Savion, S. Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines. Circulation Research. 51, (6), 787-801 (1982).
5. Rohr, S., Flückiger-Labrada, R., Kucera, J. P. Photolithographically defined deposition of attachment factors as a versatile method for patterning the growth of different cell types in culture. Pflügers Archive: European Journal of Physiology. 446, (1), 125-132 (2003).
6. Zhang, B., Green, J. V., Murthy, S. K., Radisic, M. Label-free enrichment of functional cardiomyocytes using microfluidic deterministic lateral flow displacement. PloS One. 7, (5), e37619 (2012).
7. Boateng, S. Y., Hartman, T. J., Ahluwalia, N., Vidula, H., Desai, T. Inhibition of fibroblast proliferation in cardiac myocyte cultures by surface microtopography. American Journal of Physiology. Cell Physiology. 285, (1), C171-C182 (2003).
8. Fu, J. -D., Li, J., et al. Crucial role of the sarcoplasmic reticulum in the developmental regulation of Ca2+ transients and contraction in cardiomyocytes derived from embryonic stem cells. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology. 20, (1), 181-183 (2006).
9. Liu, J., Fu, J. D., Siu, C. W., Li, R. a Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation. Stem Cells. 25, (12), 3038-3044 (2007).
10. Knollmann, B. C. Induced pluripotent stem cell-derived cardiomyocytes: boutique science or valuable arrhythmia model. Circulation Research. 112, (6), 969-976 (2013).
11. Uchida, T., Nagayama, M., Taira, T., Shimizu, K., Sakai, M., Gohara, K. Optimal temperature range for low-temperature preservation of dissociated neonatal rat cardiomyocytes. Cryobiology. 63, (3), 279-284 (2011).
12. Miyamura, K., Nagayama, M., et al. Evaluation of viability of cryopreserved rat cardiac myocytes and effects of dimethyl sulfoxide concentration on cryopreservation. Cryobiology. 59, (3), 375 (2010).
13. Rana, P., Anson, B., Engle, S., Will, Y. Characterization of human-induced pluripotent stem cell-derived cardiomyocytes: bioenergetics and utilization in safety screening. Toxicological Sciences: An Official Journal of the Society of Toxicology. 130, (1), 117-131 (2012).
14. Yokomuro, H., Mickle, D. aG., Weisel, R. D., Li, R. -K. Optimal conditions for heart cell cryopreservation for transplantation. Molecular and Cellular Biochemistry. 242, (1-2), 109-114 (2003).
15. Simpson, P., McGrath, A, Savion, S. Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines. Circulation Research. 51, (6), 787-801 (1982).
16. Evans, H. J., Western Goodwin, R. L. Array analysis of cell cycle protein changes during the hyperplastic to hypertrophic transition in heart development. Molecular and Cellular Biochemistry. 303, (1-2), 189-199 (2007).
17. Golden, H. B., Gollapudi, D., et al. Methods in Molecular Biology. 205-214 (2012).
18. Zlochiver, S., Muñoz, V., Vikstrom, K. L., Taffet, S. M., Berenfeld, O., Jalife, J. Electrotonic myofibroblast-to-myocyte coupling increases propensity to reentrant arrhythmias in two-dimensional cardiac monolayers. Biophysical Journal. 95, (9), 4469-4480 (2008).
19. Chang, M. G., Zhang, Y., et al. Spiral waves and reentry dynamics in an in vitro model of the healed infarct border zone. Circulation Research. 105, (11), 1062-1071 (2009).