Defining human dendritic cell progenitors by multiparametric flow cytometry

Nature Protocols

Volumn 10, Issue 9, 2015, Pages 1407-1422

  Breton, Gaëlle ^a   Lee, Jaeyop ^b   Liu, Kang ^b   Nussenzweig, Michel C ^a,c  

^a ROCKEFELLER UNIVERSITY   (United States)

^b NewYork Presbyterian Hospital   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD115 ANTIGEN; CD116 ANTIGEN; CD123 ANTIGEN; CD135 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; CD45RA ANTIGEN; CELL MARKER; MONOCLONAL ANTIBODY; STEM CELL FACTOR RECEPTOR; UNCLASSIFIED DRUG;

AGED; ANIMAL CELL; ARTICLE; BONE MARROW; BONE MARROW STROMA CELL; CELL DIFFERENTIATION; CELL ISOLATION; CELL PROLIFERATION; CELL SELECTION; COMMON DENDRITIC PROGENITOR CELL; CONTROLLED STUDY; DENDRITIC CELL; DENDRITIC PRECURSOR CELL; FLOW CYTOMETRY; FREEZE THAWING; GRANULOCYTE MONOCYTE AND DENDRITIC PROGENITOR CELL; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MONOCYTE AND DENDRITIC PROGENITOR CELL; MONONUCLEAR CELL; MOUSE; MULTIPARAMETRIC FLOW CYTOMETRY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; STEM CELL; UMBILICAL CORD BLOOD; BONE MARROW CELL; CYTOLOGY; PHYSIOLOGY; PROCEDURES;

BONE MARROW CELLS; CELL DIFFERENTIATION; DENDRITIC CELLS; FLOW CYTOMETRY; HUMANS; STEM CELLS;

EID: 84940552348     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.092     Document Type: Article

References (24)

1. Steinman, R.M. & Hemmi, H. Dendritic cells: translating innate to adaptive immunity. Curr. Top. Microbiol. Immunol. 311, 17-58 (2006).
2. Steinman, R.M. & Banchereau, J. Taking dendritic cells into medicine. Nature 449, 419-426 (2007).
3. Manicassamy, S. & Pulendran, B. Dendritic cell control of tolerogenic responses. Immunol. Rev. 241, 206-227 (2011).
4. Colonna, M., Trinchieri, G. & Liu, Y.J. Plasmacytoid dendritic cells in immunity. Nat. Immunol. 5, 1219-1226 (2004).
5. Jin, J.O., Zhang, W., Du, J.Y. & Yu, Q. BDCA1-positive dendritic cells (DCs) represent a unique human myeloid DC subset that induces innate and adaptive immune responses to Staphylococcus aureus infection. Infect. Immun. 82, 4466-4476 (2014).
6. Cohn, L. et al. Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3+ dendritic cells at cross presentation. J. Exp. Med. 210, 1049-1063 (2013).
7. + dendritic cells. J. Exp. Med. 207, 1273-1281 (2010).
8. + dendritic cells. J. Exp. Med. 207, 1283-1292 (2010).
9. + dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. J. Exp. Med. 207, 1247-1260 (2010).
10. + dendritic cells. J. Exp. Med. 207, 1261-1271 (2010).
11. + dendritic cells. J. Exp. Med. 207, 1131-1134 (2010).
12. Robbins, S.H. et al. Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profling. Genome Biol. 9, R17 (2008).
13. Geissmann, F. et al. Development of monocytes, macrophages, and dendritic cells. Science 327, 656-661 (2010).
14. Lee, J. et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J. Exp. Med. 212, 385-99 (2015).
15. + dendritic cells. J. Exp. Med. 212, 401-413 (2015).
16. Doulatov, S. et al. Revised map of the human progenitor hierarchy shows the origin of macrophages and dendritic cells in early lymphoid development. Nat. Immunol. 11, 585-593 (2010).
17. Hao, Q.L. et al. Identifcation of a novel, human multilymphoid progenitor in cord blood. Blood 97, 3683-3690 (2001).
18. + common lymphoid progenitors expressing lymphoid-specifc genes. Leukemia 21, 311-319 (2007).
19. Majeti, R., Park, C.Y. & Weissman, I.L. Identifcation of a hierarchy of multipotent hematopoietic progenitors in human cord blood. Cell Stem Cell 1, 635-645 (2007).
20. - cells assured by intra-bone marrow injection. Blood 101, 2924-2931 (2003).
21. Boissel, N. et al. Defective blood dendritic cells in chronic myeloid leukemia correlate with high plasmatic VEGF and are not normalized by imatinib mesylate. Leukemia 18, 1656-1661 (2004).
22. Bigley, V. et al. The human syndrome of dendritic cell, monocyte, B and NK lymphoid defciency. J. Exp. Med. 14, 227-234 (2011).
23. Hambleton, S. et al. IRF8 mutations and human dendritic-cell immunodefciency. N. Engl. J. Med. 365, 127-138 (2011).
24. Perfetto, S.P. et al. Quality assurance for polychromatic fow cytometry using a suite of calibration beads. Nat. Protoc. 7, 2067-2079 (2012).