Identification of novel genes associated with alginate production in Pseudomonas aeruginosa using Mini-himar1 mariner transposon-mediated mutagenesis

Journal of Visualized Experiments

Volumn , Issue 85, 2014, Pages

  Ryan Withers, T ^a   Yin, Yeshi ^a   Yu, Hongwei D ^a  

^a MARSHALL UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Alginate; Immunology; Issue 85; Mini himar1 mariner transposon; Mucoidy; Mutagenesis; pFAC; Pseudomonas aeruginosa

Indexed keywords

EID: 84940282305     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51346     Document Type: Article

References (16)

1. Govan, J. R. & Deretic, V. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 60, 539-574 (1996).
2. Chitnis, C. E. & Ohman, D. E. Genetic analysis of the alginate biosynthetic gene cluster of Pseudomonas aeruginosa shows evidence of an operonic structure. Mol. Microbiol. 8, 583-593 (1993).
3. Franklin, M. J. et al. Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase. J. Bacteriol. 176, 1821-1830 (1994).
4. Franklin, M. J. & Ohman, D. E. Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate inPseudomonas aeruginosa. J. Bacteriol. 184, 3000-3007 (2002).
5. Deretic, V., Gill, J. F. & Chakrabarty, A. M. Gene algD coding for GDPmannose dehydrogenase is transcriptionally activated in mucoid Pseudomonas aeruginosa. J. Bacteriol. 169, 351-358 (1987).
6. Mathee, K., McPherson, C. J. & Ohman, D. E. Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon inPseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN). J. Bacteriol. 179, 3711-3720 (1997).
7. Boucher, J. C., Schurr, M. J., Yu, H., Rowen, D. W. & Deretic, V. Pseudomonas aeruginosa in cystic fibrosis: role of mucC in the regulation of alginate production and stress sensitivity. Microbiology. 143, 3473-3480 (1997).
8. Martin, D. W., Holloway, B. W. & Deretic, V. Characterization of a locus determining the mucoid status of Pseudomonas aeruginosa: AlgU shows sequence similarities with a Bacillus sigma factor. J. Bacteriol. 175, 1153-1164 (1993).
9. Damron, F. H. & Goldberg, J. B. Proteolytic regulation of alginate overproduction in Pseudomonas aeruginosa. Mol. Microbiol. 84 (4), 595-607 (2012).
10. Browne, P., Barret, M., O'Gara, F. & Morrissey, J. P. Computational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria. BMC Microbiol. 10, 300 (2010).
11. Damron, F. H., Qiu, D. & Yu, H. D. The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis. J. Bacteriol. 191, 2285-2295 (2009).
12. Damron, F. H. et al. Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN. J. Bacteriol. 194, 1317-1330 (2012).
13. Qiu, D., Eisinger, V. M., Rowen, D. W. & Yu, H. D. Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U.S.A. 104, 8107-8112 (2007).
14. Schurr, M. J. Which bacterial biofilm exopolysaccharide is preferred, Psl or alginate? J. Bacteriol. 195, 1623-1626 (2013).
15. Wong, S. M. & Mekalanos, J. J. Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U.S.A. 97, 10191-10196 (2000).
16. Lampe, D. J., Akerley, B. J., Rubin, E. J., Mekalanos, J. J. & Robertson, H. M. Hyperactive transposase mutants of the Himar1 mariner transposon. Proc. Natl. Acad. Sci. U.S.A. 96, 11428-11433 (1999).