Comparing two intestinal porcine epithelial cell lines (IPECs): Morphological differentiation, function and metabolism

PLoS ONE

Volumn 10, Issue 7, 2015, Pages

  Nossol, Constanze ^a   Barta Böszörményi, Anicò ^a   Kahlert, Stefan ^a   Zuschratter, Werner ^b   Faber Zuschratter, Heidi ^a   Reinhardt, Nicole ^a   Ponsuksili, Siriluk ^c   Wimmers, Klaus ^c   Diesing, AnneKathrin ^a   Rothkötter, Hermann Josef ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUTE FOR NEUROBIOLOGY   (Germany)

^c LEIBNIZ INSTITUTE FOR FARM ANIMAL BIOLOGY FBN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ACTIN FILAMENT; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL FUNCTION; CELL METABOLISM; CELL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DOWN REGULATION; ELECTRIC RESISTANCE; GENE EXPRESSION; GENETIC ASSOCIATION; ILEUM; IMMUNOFLUORESCENCE; IN VITRO STUDY; INTESTINE EPITHELIUM CELL; INTESTINE FUNCTION; JEJUNUM; MICROARRAY ANALYSIS; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PIG; PROTEIN CONTENT; RIBOSOME; RNA DEGRADATION; SPLICEOSOME; TIGHT JUNCTION; TRANSMISSION ELECTRON MICROSCOPY; UPREGULATION; ANIMAL; CELL LINE; CELL SHAPE; COMPARATIVE STUDY; CYTOLOGY; EPITHELIUM CELL; INTESTINE; INTESTINE MUCOSA; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY;

ANIMALIA; SUIDAE; SUS;

ANIMALS; CELL DIFFERENTIATION; CELL LINE; CELL SHAPE; EPITHELIAL CELLS; INTESTINAL MUCOSA; INTESTINES; PHOSPHORYLATION; SWINE; UP-REGULATION;

EID: 84940056629     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0132323     Document Type: Article

References (31)

1. Litten-Brown J.C., Corson AM and Clarke L. Porcine models forthe metabolic syndrome, digestive and bone disorders: a general overview. Animal 2010 4:899-920 doi: 10.1017/S1751731110000200 PMID: 22444262
2. Lamendella R, Domingo JWS, Ghosh S, Martinson J and Oerther DB. Comparative fecal metagenomics unveils unique functional capacity of the swine gut. BMC Microbiol 2011 11: 103. doi: 10.1186/1471-2180-11-103 PMID: 21575148
3. Chantret I, Barbat A, Dussaulx E., Brattain MG, Zweibaum A. Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells, a survey of twenty cell lines. Cancer Res 1988 48:1936-1942. PMID: 3349466
4. Chantret I, Rodolosse A, Barbat A., Dussaulx E, Brot-Laroche E, Zweibaum A, et al Differential expression of sucrase-isomaltase in clones isolated from early and late passages of the cell lineCaco-2, evidence for glucose-dependent negative regulation. J Cell Sci 1994 107(Pt 1):213-225.
5. Hidalgo IJ, Li JB Carrier-mediated transport and efflux mechanisms in Caco-2 cells. Advanced Drug Delivery Reviews 1996 22:53-66.
6. Basora N, Desloges N, Chang Q., Bouatrouss Y, Gosselin J, Poisson J., et al. Expression of the alpha9-beta1 integrin in human colonic epithelial cells, resurgence of the fetal phenotype in a subset of colon cancers and adenocarcinoma cell lines. Int J Cancer 1998 75:738-743. PMID: 9495242
7. Schierack P, Nordhoff M, Pollmann M., Weyrauch KD, Amasheh S, et al. (2006) Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine. Histochem Cell Biol 125: 293-305. doi: 10.1007/s00418-005-0067-z PMID: 16215741
8. Diesing AK, Nossol C, Ponsuksili S, Wimmers K., Kluess J, Walk N, et al Gene Regulation of Intestinal Porcine Epithelial Cells IPECJ2 is Dependent on the Site of Deoxynivalenol Toxicological Action. Plo-SOne 2012 7(4):e34136. doi:10.1371/journal.pone.0034136
9. Berschneider H. Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl. Abstract of the Annual Meeting of the American Gastroenterological Association 1989.
10. Steube K, Koelz AL, Uphoff C, Drexler H.G., Kluess J., Steinberg P. The necessity of identity assessment of animal intestinal cell lines: A case report. Cytotechnology 2011 doi: 10.1007/s10616-011-9420-3
11. Bolstad BM, Irizarry RA, Astrand M, Speed TP A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 2003 19:185-193. PMID: 12538238
12. Dennis G Jr., Sherman BT, Hosack DA, Yang J, Gao W, et al. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol 2003 4:3.
13. Nossol C, Diesing AK, Walk N, Faber-Zuschratter H, Hartig R., Post A, Kluess J, et al Air-liquid interface cultures enhance the oxygen supply and triggerthe structural and functional differentiation of intestinal porcine epithelial cells (IPEC). Histochem Cell Biol 2011 136:103-115. doi: 10.1007/s00418-011-0826-y PMID: 21681518
14. Quaroni A, Wands J, Trelstad R.L., Isselbacher KJ Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J Cell Biol 1979 80:248-265. PMID: 88453
15. Gonzalez-Vallina R., Wang H, Zhan R., Berschneider HM, Lee RM, Davidson NO, et al Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1). Am J Physiol 1996 271: G249-G259. PMID: 8770040
16. Diesing AK, Nossol C, Dänicke S, Walk N, Post A, Kahlert S, Rothkötter HJ, et al. Vulnerability of polarised intestinal porcine epithelial cells to mycotoxin deoxynivalenol depends on the route of application. PLoS One 2011 6:e17472. doi: 10.1371/journal.pone.0017472 PMID: 21364771
17. Sargeant HR, Shaw MA, Abu Oun M, Collins J.W., Woodward MJ, La Ragione RM, et al. The metabolic impact of zinc oxide on porcine intestinal cells and enterotoxigenic Escherichia coli K88. Livestock Science 2010 133(1-3):45-48.
18. MacPherson I, Montagnier. Agar suspension culture forthe selective assay of cells transformed by polyomavirus. Virology 1964 23:291-294. PMID: 14187925
19. Frisch SM, Screaton RA Anoikis mechanisms. CurrOpin Cell Biol 2001 13:555-562. PMID: 11544023
20. Cerda SR, Bissonnette M, Scaglione-Sewell B, Lyons M.R., Khare S., Mustafi R, et al. PKC-delta inhibits anchorage-dependent and - independent growth, enhances differentiation, and increases apoptosis in CaCo-2 cells. Gastroenterology 2001 120:1700-1712. PMID: 11375951
21. Mooseker MS. Actin binding proteins of the brush border. Cell 1983 35:11-3. PMID: 6313218
22. Brown JB, McKnight CJ Molecular model of the microvillarcytoskeleton and organisation of the brush border. PLoSONE 2010 5:e9406.
23. Macleod KF, Sherry N, Hannon G., Beach D, Tokino T, Kinzler K., et al. P53-Dependentand Independent Expression of P21 During Cell-Growth, Differentiation, and Dna-Damage. Genes & Development 1995 9:935-944.
24. Cox LS, Lane DP Tumor Suppressors, Kinases and Clamps - How P53 Regulates the Cell-Cycle in Response to Dna-Damage. Bioessays 1995 17:501-508. PMID: 7575491
25. Bargou RC, Daniel PT, Mapara M.Y., Bommert K., Wagener C, Kallinich B., et al. Expression of the bcl-2 gene family in normal and malignant breast tissue: low bax-alpha expression in tumor cells correlates with resistance towards apoptosis. Int J Cancer 1995 16: 60(6):854-9. PMID: 7896458
26. Miyashita T, Krajewski S, Krajewska M., Wang HG, Lin HK, Liebermann DA, et al Tumor suppressor p53isa regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene 1994 9(6):1799-805. PMID: 8183579
27. Jiang P, Du WJ, Heese K, Wu M. The bad guy cooperates with good cop p53, Bad is transcriptionally up-regulated by p53 and forms a bad/p53 complex at the mitochondria to induce apoptosis. Molecular and Cellular Biology 2006 26:9071-9082. PMID: 17000778
28. Niessen CM, Gottardi CJ Molecular components of the adherens junctions. Biochimicaet Biophysica Acta 2008 1778:562-571. doi: 10.1016/j.bbamem.2007.12.015 PMID: 18206110
29. Matter K, Balda MS Functional analysis of tight junctions. Methods 2003 30:228-234. PMID: 12798137
30. Zagari F, Jordan M, Stettler M., Broly H, Wurm F. Lactate metabolism shift in CHO cells: the role of mitochondrial oxidative activity. New Biotechnology 2013 30(2):238-245. doi: 10.1016/j.nbt.2012.05.021 PMID: 22683938
31. Sureshkumar GK, Mtharasan R. The influence of temperature on a mose-mouse hybridoma growth and monoclonal antibody production. Biotechnol Bioeng 1991 55: 328-338.