Genetic characterisation of clinical Klebsiella pneumoniae isolates with reduced susceptibility to tigecycline: Role of the global regulator RamA and its local repressor RamR

International Journal of Antimicrobial Agents

Volumn 45, Issue 6, 2015, Pages 635-640

  Wang, Xiaojuan ^a   Chen, Hongbin ^a   Zhang, Yawei ^a   Wang, Qi ^a   Zhao, Chunjiang ^a   Li, Henan ^a   He, Wenqiang ^a   Zhang, Feifei ^a   Wang, Zhanwei ^a   Li, Shuguang ^a   Wang, Hui ^a  

^a PEKING UNIVERSITY PEOPLE S HOSPITAL   (China)

Author keywords

Klebsiella pneumoniae; marA; ramA; ramR; Tigecycline

Indexed keywords

ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; MINOCYCLINE; RAMA PROTEIN, KLEBSIELLA; TIGECYCLINE; TRANSACTIVATOR PROTEIN; TRANSCRIPTOME;

ANALOGS AND DERIVATIVES; ANTIBIOTIC RESISTANCE; DRUG EFFECTS; GENE INACTIVATION; GENETIC COMPLEMENTATION; HUMAN; ISOLATION AND PURIFICATION; KLEBSIELLA INFECTION; KLEBSIELLA PNEUMONIAE; METABOLISM; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REGULON;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DRUG RESISTANCE, BACTERIAL; GENE KNOCKOUT TECHNIQUES; GENETIC COMPLEMENTATION TEST; HUMANS; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; MICROBIAL SENSITIVITY TESTS; MINOCYCLINE; POLYMERASE CHAIN REACTION; REGULON; TRANS-ACTIVATORS; TRANSCRIPTOME;

EID: 84939984183     PISSN: 09248579     EISSN: 18727913     Source Type: Journal    
DOI: 10.1016/j.ijantimicag.2014.12.022     Document Type: Article

References (15)

1. Y. Sun, Y. Cai, X. Liu, N. Bai, B.B. Liang, and R. Wang The emergence of clinical resistance to tigecycline Int J Antimicrob Agents 41 2013 110 116
2. D. Keeney, A. Ruzin, and P.A. Bradford RamA, a transcriptional regulator, and AcrAB, a RND-type efflux pump, are associated with decreased susceptibility to tigecycline in Enterobacter cloacae Microb Drug Resist 13 2007 1 6
3. D. Keeney, A. Ruzin, F. McAleese, M.E. Murphy, and P.A. Bradford MarA-mediated overexpression of the AcrAB efflux pump results in decreased susceptibility to tigecycline in Escherichia coli J Antimicrob Chemother 61 2008 46 53
4. T. Horiyama, E. Nikaido, A. Yamaguchi, and K. Nishino Roles of Salmonella multidrug efflux pumps in tigecycline resistance J Antimicrob Chemother 66 2011 105 110
5. Z.K. Sheng, F. Hu, W. Wang, Q. Guo, Z. Chen, X. Xu, et al. Mechanisms of tigecycline resistance among Klebsiella pneumoniae clinical isolates Antimicrob Agents Chemother 58 2014 6982 6985
6. T. Schneiders, S.G.B. Amyes, and S.B. Levy Role of AcrR and RamA in fluoroquinolone resistance in clinical Klebsiella pneumoniae isolates from Singapore Antimicrob Agents Chemother 47 2003 2831 2837
7. A. Ruzin, F.W. Immermann, and P.A. Bradford Real-time PCR and statistical analyses of acrAB and ramA expression in clinical isolates of Klebsiella pneumoniae Antimicrob Agents Chemother 52 2008 3430 3432
8. S. Bratu, D. Landman, A. George, J. Salvani, and J. Quale Correlation of the expression of acrB and the regulatory genes marA, soxS and ramA with antimicrobial resistance in clinical isolates of Klebsiella pneumoniae endemic to New York City J Antimicrob Chemother 64 2009 278 283
9. M. Hentschke, M. Wolters, I. Sobottka, I. Rohde, and M. Aepfelbacher ramR mutation in clinical isolates of Klebsiella pneumoniae with reduced susceptibility to tigecycline Antimicrob Agents Chemother 54 2010 2720 2723
10. R. Rosenblum, E. Khan, G. Gonzalez, R. Hasan, and T. Schneiders Genetic regulation of the ramA locus and its expression in clinical isolates of Klebsiella pneumoniae Int J Antimicrob Agents 38 2011 39 45
11. L. Villa, C. Feudi, D. Fortini, A. García-Fermández, and A. Carattoli Genomics of KPC-producing Klebsiella pneumoniae sequence type 512 clone highlights the role of RamR and ribosomal S10 protein mutations in conferring tigecycline resistance Antimicrob Agents Chemother 58 2014 1707 1712
12. Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing: twenty-third informational supplement Document M100-S23 2013 CLSI Wayne, PA
13. S. Bialek-Davenet, E. Marcon, V. Leflon-Guibout, J.P. Lavigne, F. Bert, R. Moreau, et al. In vitro selection of ramR and soxR mutants overexpressing efflux systems by fluoroquinolones as well as cefoxitin in Klebsiella pneumoniae Antimicrob Agents Chemother 55 2011 2795 2802
14. Y.J. Pan, H.C. Fang, H.C. Yang, T.L. Lin, P.F. Hsieh, F.C. Tsai, et al. Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype J Clin Microbiol 46 2008 2231 2240
15. E. Voulgari, A. Poulou, V. Koumaki, and A. Tsakris Carbapenemase-producing Enterobacteriaceae: now that the storm is finally here, how will timely detection help us fight back? Future Microbiol 8 2013 27 39