The challenge of on-tissue digestion for MALDI MSI— a comparison of different protocols to improve imaging experiments

Analytical and Bioanalytical Chemistry

Volumn 407, Issue 8, 2021, Pages

  Diehl, Hanna C ^a   Beine, Birte ^a,b   Elm, Julian ^a   Trede, Dennis ^c   Ahrens, Maike ^a   Eisenacher, Martin ^a   Marcus, Katrin ^a   Meyer, Helmut E ^b   Henkel, Corinna ^a,b  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b LEIBNIZ INSTITUT FÜR ANALYTISCHE WISSENSCHAFTEN ISAS E V   (Germany)

^c UNIVERSITY OF BREMEN   (Germany)

Author keywords

Formalin fixed paraffin embedded (FFPE); LysC mix; Mass spectrometry imaging (MSI); Matrix assisted laser desorption ionization (MALDI); Peptide imaging; Sample preparation; Tryptic digestion

Indexed keywords

DESORPTION; DRUG PRODUCTS; FORMALDEHYDE; IMAGING SYSTEMS; MASS SPECTROMETRY; PARAFFINS; PEPTIDES; QUALITY CONTROL; SEMANTICS; TISSUE;

DIFFERENT PROTOCOLS; EXPERIMENTAL EVALUATION; MASS SPECTROMETRY IMAGING (MSI); MATRIX ASSISTED LASER DESORPTION/IONIZATION; MYELIN BASIC PROTEIN; PROBABILISTIC LATENT SEMANTIC ANALYSIS; SAMPLE PREPARATION; TRYPTIC DIGESTION;

MOLECULAR IMAGING;

PEPTIDE; PROTEIN;

ANIMAL; BRAIN CHEMISTRY; CHEMISTRY; COMPARATIVE STUDY; DIGESTION; HISTOLOGY; MALE; MASS SPECTROMETRY; MICROTOMY; PROCEDURES; PROTEOMICS; RAT; WISTAR RAT;

ANIMALS; BRAIN CHEMISTRY; DIGESTION; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; MALE; MICROTOMY; PEPTIDES; PROTEINS; PROTEOMICS; RATS; RATS, WISTAR; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84938940986     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-8345-z     Document Type: Article

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