The association of Panton-Valentine leukocidin and mecA genes in methicillin-resistant staphylococcus aureus isolates from patients referred to educational hospitals in Ahvaz, Iran

Jundishapur Journal of Microbiology

Volumn 8, Issue 8, 2015, Pages

  Motamedi, Hossein ^a   Abadi, Seyyed Soheil Rahmat ^a   Moosavian, Seyyed Mojtaba ^b   Torabi, Maryam ^b  

^a SHAHID CHAMRAN UNIVERSITY OF AHVAZ   (Iran)

^b AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Iran; Methicillin resistance; MRSA; Panton valentine leukocidin; Virulence factor

Indexed keywords

ANTIBIOTIC AGENT; BACITRACIN; CATALASE; COAGULASE; DNA; FURAZOLIDONE; MANNITOL; METICILLIN; OXACILLIN; OXIDOREDUCTASE; PANTON VALENTINE LEUKOCIDIN; PENICILLIN BINDING PROTEIN 2A;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM ISOLATION; CONTROLLED STUDY; DISK DIFFUSION; DNA EXTRACTION; DRUG SCREENING; FEMALE; FERMENTATION; GENETIC ASSOCIATION; GENETIC IDENTIFICATION; GENETIC MARKER; HUMAN; IRAN; MALE; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PATIENT REFERRAL; PROTEIN ANALYSIS; PVL GENE;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 84938810174     PISSN: 20083645     EISSN: 20084161     Source Type: Journal    
DOI: 10.5812/jjm.22021v2     Document Type: Article

References (30)

1. David MZ, Daum RS. Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clin Microbiol Rev. 2010;23(3):616–87
2. Dash N, Panigrahi D, Al Zarouni M, Yassin F, Al-Shamsi M. Incidence of community-acquired methicillin-resistant Staphylococcus aureus carrying Pantone-Valentine leucocidin gene at a referral hospital in United Arab Emirates. APMIS. 2014; 122(4):341–6
3. Sanchini A, Del Grosso M, Villa L, Ammendolia MG, Superti F, Monaco M, et al. Typing of Panton-Valentine leukocidin-encoding phages carried by methicillin-susceptible and methicillinresistant Staphylococcus aureus from Italy. Clin Microbiol Infect. 2014;20(11):O840–6
4. Carleton HA, Diep BA, Charlebois ED, Sensabaugh GF, Perdreau- Remington F. Community-adapted methicillin-resistant Staphylococcus aureus (MRSA): population dynamics of an expanding community reservoir of MRSA. J Infect Dis. 2004;190(10):1730–8
5. Srinivasan A, Seifried S, Zhu L, Srivastava DK, Flynn PM, Shenep JL, et al. Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus infections in children with cancer. Pediatr Blood Cancer. 2009;53(7):1216–20
6. Khosravi AD, Hoveizavi H, Farshadzadeh Z. The prevalence of genes encoding leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in Taleghani Hospital, Ahvaz, Iran. Burns. 2012;38(2):247–51
7. Los FC, Randis TM, Aroian RV, Ratner AJ. Role of pore-forming toxins in bacterial infectious diseases. Microbiol Mol Biol Rev. 2013;77(2):173–207
8. Yanagihara K, Kihara R, Araki N, Morinaga Y, Seki M, Izumikawa K, et al. Efficacy of linezolid against Panton-Valentine leukocidin (PVL)-positive meticillin-resistant Staphylococcus aureus (MRSA) in a mouse model of haematogenous pulmonary infection. Int J Antimicrob Agents. 2009;34(5):477–81
9. Terry Alli OA, Ogbolu DO, Mustapha JO, Akinbami R, Ajayi AO. The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus aureus from hospitals in South Western Nigeria. Indian J Med Microbiol. 2012;30(2):159–64
10. Kim MH, Lee WI, Kang SY. Detection of methicillin-resistant Staphylococcus aureus in healthcare workers using real-time polymerase chain reaction. Yonsei Med J. 2013;54(5):1282–4
11. Ba X, Harrison EM, Edwards GF, Holden MT, Larsen AR, Petersen A, et al. Novel mutations in penicillin-binding protein genes in clinical Staphylococcus aureus isolates that are methicillin resistant on susceptibility testing, but lack the mec gene. J Antimicrob Chemother. 2014;69(3):594–7
12. van der Meeren BT, Millard PS, Scacchetti M, Hermans MH, Hilbink M, Concelho TB, et al. Emergence of methicillin resistance and Panton-Valentine leukocidin positivity in hospital- and community-acquired Staphylococcus aureus infections in Beira, Mozambique. Trop Med Int Health. 2014;19(2):169–76
13. Neela V, Ehsanollah GR, Zamberi S, Van Belkum A, Mariana NS. Prevalence of Panton-Valentine leukocidin genes among carriage and invasive Staphylococcus aureus isolates in Malaysia. Int J Infect Dis. 2009;13(3):e131–2
14. Chen FJ, Hiramatsu K, Huang IW, Wang CH, Lauderdale TL. Panton- Valentine leukocidin (PVL)-positive methicillin-susceptible and resistant Staphylococcus aureus in Taiwan: identification of oxacillin-susceptible mecA-positive methicillin-resistant S. aureus. Diagn Microbiol Infect Dis. 2009;65(4):351–7
15. Tile PM. Bailey & Scott’s Diagnostic Microbiology.New York: Mosby; 2013
16. Clinical and laboratory standard institute.. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Wayne; 2006
17. Clinical and laboratory standard institute.. Performance standards for antimicrobial susceptibility testing; Twenty second informational supplement. Wayne; 2012
18. Sambrookm J, Fritsch EF, Maniatis T. Molecular cloning, A Laboratorry Manual.New York: Cold Spring Harbor Laboratory Press; 1989
19. McClure JA, Conly JM, Lau V, Elsayed S, Louie T, Hutchins W, et al. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. J Clin Microbiol. 2006;44(3):1141–4
20. Shore AC, Tecklenborg SC, Brennan GI, Ehricht R, Monecke S, Coleman DC. Panton-Valentine leukocidin-positive Staphylococcus aureus in Ireland from 2002 to 2011: 21 clones, frequent importation of clones, temporal shifts of predominant methicillinresistant S. aureus clones, and increasing multiresistance. J Clin Microbiol. 2014;52(3):859–70
21. Bagdonas R, Tamelis A, Rimdeika R, Kiudelis M. Analysis of burn patients and the isolated pathogens. Lithuanian Surg. 2004; 2(3):190–3
22. Turnidge JD, Nimmo GR, Pearson J, Gottlieb T, Collignon PJ, Australian Group on Antimicrobial R. Epidemiology and outcomes for Staphylococcus aureus bacteraemia in Australian hospitals, 2005-06: report from the Australian Group on Antimicrobial Resistance. Commun Dis Intell Q Rep. 2007;31(4):398–403
23. Li J, Zeng H, Xu X. [A study of methicillin -- resistant staphylococcus aureus (MRSA) in a burn unit with repetitive -- DNA -- sequence -- based PCR fingerprinting]. Zhonghua Shao Shang Za Zhi. 2001;17(2):88–90
24. Fatholahzadeh B, Emaneini M, Aligholi M, Gilbert G, Taherikalani M, Jonaidi N, et al. Molecular characterization of methicillin-resistant Staphylococcus aureus clones from a teaching hospital in Tehran. Jpn J Infect Dis. 2009;62(4):309–11
25. Japoni A, Alborzi A, Orafa F, Rasouli M, Farshad S. Distribution patterns of methicillin resistance genes (mecA) in Staphylococcus aureus isolated from clinical specimens. Iran Biomed J. 2004;8(4):173–8
26. Melles DC, Gorkink RF, Boelens HA, Snijders SV, Peeters JK, Moorhouse MJ, et al. Natural population dynamics and expansion of pathogenic clones of Staphylococcus aureus. J Clin Invest. 2004;114(12):1732–40
27. Okon KO, Basset P, Uba A, Lin J, Oyawoye B, Shittu AO, et al. Cooccurrence of predominant Panton-Valentine leukocidin-positive sequence type (ST) 152 and multidrug-resistant ST 241 Staphylococcus aureus clones in Nigerian hospitals. J Clin Microbiol. 2009;47(9):3000–3
28. Affolabi D, Odoun M, Faïhun F, Damala RL, Ahovegbe L, Prudence Wachinou A. Non-association of the presence of panton-valentine leukocidin gene with antimicrobial-resistance in Staphylococcus aureus isolates in Cotonou, Benin. Int J of Curr Res. 2014;6(1):4617–20
29. San Juan JA, Cabugao DBG, Dabbay B, Cabrera EC. Occurrence of Methicillin-Resistant Staphylococcus aureus (MRSA) among the Health Workers of Rizal Provincial Hospital and Characterization for the Presence of luks-lukf PVL Gene. Philippine J Sci. 2012;141(2):157–63
30. Rebollo-Perez J, Ordonez-Tapia C, Herazo-Herazo C, Reyes-Ramos N. Nasal carriage of Panton Valentine leukocidin-positive methicillin- resistant Staphylococcus aureus in healthy preschool children. Rev Salud Publica (Bogota). 2011;13(5):824–32.