Tobacco Chloroplasts as Bioreactors for the Production of Recombinant Superoxide Dismutase in Plants, an Industrially Useful Enzyme

Plant Molecular Biology Reporter

Volumn 33, Issue 4, 2015, Pages 1107-1115

  Madanala, Raju ^a   Gupta, Vijayta ^b   Pandey, Ashutosh Kumar ^a   Srivastava, Subhi ^a   Pandey, Vivek ^a   Singh, Pradhyumna Kumar ^a   Tuli, Rakesh ^c  

^a CSIR NATIONAL BOTANICAL RESEARCH INSTITUTE   (India)

^b CSIR CENTRAL INSTITUTE OF MEDICINAL AND AROMATIC PLANTS   (India)

^c NATIONAL AGRI FOOD BIOTECHNOLOGY INSTITUTE   (India)

Author keywords

Chloroplast transformation; Cu Zn SOD; Recombinant proteins; Tobacco; Transplastomic plants

Indexed keywords

NICOTIANA TABACUM; WITHANIA SOMNIFERA;

EID: 84938746704     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-014-0805-2     Document Type: Article

References (37)

1. Bafana A, Dutt S, Kumar S, Ahuja PS (2010) Superoxide dismutase: an industrial perspective. Crit Rev Biotechnol 31:65–76
2. Bally J, Paget E, Droux M, Job C, Dubald M (2008) Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins. Plant Biotechnol J 6:46–61
3. Barnes D, Franklin S, Schultz J, Henry R, Brown E, Coragliotti A, Mayfield SP (2005) Contribution of 5′ and 3′ untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes. Mol Gen Genomics 274:625–636
4. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
5. Bock R (2013) Strategies for metabolic pathway engineering with multiple transgenes. Plat Mol Biol 83:21–31
6. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
7. Chen C-N, Pan S-M (1996) Assay of superoxide dismutase activity by combining electrophoresis and densitometry. Bot Bull Acad Sin 37:107–111
8. Daniell H (2006) Production of biopharmaceuticals and vaccines in plants via the chloroplast genome. Biotechnol J 1:1071–1079
9. Daniell H, Cohill P, Kumar S, Dufourmantel N (2004) Chloroplast genetic engineering. In: Daniell H, Chase C (eds) In molecular biology and biotechnology of plant organelles. Springer, Verlag, pp 443–490
10. Dufourmantel N, Tissot G, Garc¸on F, Pelissier B, Dubald M (2006) Stability of soybean recombinant plastome over six generations. Transgenic Res 15:305–311
11. Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112
12. Hefferon K (2013) Plant-derived pharmaceuticals for the developing world. Biotechnol J 8:1193–1202
13. Hennig A, Bong K, Roitsch T, Warzecha H (2007) Expression of the recombinant bacterial outer surface protein A in tobacco chloroplasts leads to thylakoid localization and loss of photosynthesis. FEBS J 274:5749–5758
14. Hood EE (2002) From green plants to industrial enzymes. Enz Microb Technol 30:279–283
15. Khanna P, Staba J (1968) Antimicrobials from plant tissue cultures. Llyodia 31:180–189
16. Kolotilin I, Kaldis A, Pereira EO, Laberge S, Menassa R (2013) Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields. Biotechnol Biofuels 6:65
17. Koya V, Moayeri M, Leppla SH, Daniell H (2005) Plant based vaccine: mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge. Infect Immun 73:8266–8274
18. Kusnadi A, Nikolov G, Howard J (1997) Production of recombinant proteins in plants: practical considerations. Biotechnol Bioeng 56:473–484
19. Madanala R, Gupta V, Deeba F, Upadhyay SK, Pandey V, Singh PK, Tuli R (2011) A highly stable Cu/Zn superoxide dismutase from Withania somnifera plant: cloning, expression and characterization of recombinant protein. Biotechnol Let 33:2057–2063
20. Madanala R, Gupta V, Singh PK, Tuli R (2012) Development of chloroplast transformation vectors, and a new target region in the tobacco plastid genome. Plant Biotechnol Rep 6:77–87
21. Maliga P (2004) Plastid transformation in higher plants. Annu Rev Plant Biol 55:289–313
22. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
23. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
24. Nugent JM, Joyce SM (2005) Producing human therapeutic proteins in plastids. Curr Pharm Des 11:2459–2470
25. Peter OO, Shaukat HR (1989) A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lac2 gene in Escherichia coli. J Biolog Chem 264:16973–16976
26. Prashanth SR, Sadhasivam V, Parida A (2008) Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in Indica rice var Pusa Basmati-1 confers abiotic stress tolerance. Transgenic Res 17:281–291
27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Har:old Spring Harbor Laboratory Press, New York
28. Sawant SV, Singh PK, Tuli R (2000) Pretreatment of micropojectiles to improve the delivery of DNA in plant transformation. Biotechniques 29:246–248
29. Scharff LB, Bock R (2014) Synthetic biology in plastids. Plant J 78:783–798
30. Streatfeld SJ (2007) Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnol J 5:2–15
31. Surzycki R, Greenham K, Kitayama K, Dibal F, Wagner R, Rochaix JD, Ajam T, Surzycki S (2009) Factors effecting expression of vaccines in microalgae. Biologicals 37(3):133–138
32. Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci U S A 90:913–917
33. Tiwari S, Verma PC, Singh PK, Tuli R (2009) Plants as bioreactors for the production of vaccine antigens. Biotechnol Adv 27(4):449–67
34. Tregoning JS, Nixon P, Kuroda H, Svab Z, Clare S, Bowe F, Fairweather N, Ytterberg J, vanWijk KJ, Dougan G, Maliga P (2003) Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res 31:1174–1179
35. Tuli R, Sangwan RS, Kumar S, Bhattacharya S, Misra L, Mandal C, Raghubir R, Nath C, Trivedi PK, Tiwari SK, Mishra P, Chaturvedi P, Sangwan NS, Nair KN, Rawat AKS, Srivatsava V, Srivatsava RK, Ojha SK, Mehrotra S, Khanuja A, Suri KA (2009) A monograph, Aswagandha (Withania somnifera) a model Indian medicinal plant. (eds.) Tuli, R., Sangwan, R.S., CSIR, New Delhi, India. September, 2009. ISBN no: 978-93-80235
36. Verma D, Kanagaraj A, Jin S, Singh ND, Kolattukudy PE, Daniell H (2010) Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars. Plant Biotechnol J 8:332–350
37. Yarbakht M, Jalali-Javaran M, Nikkhah M, Mohebodini M (2014) Dicistronic expression of human proinsulin-protein A fusion in tobacco chloroplast. Biotechnol Appl Biochem. doi:10.1002/bab.1230