High-speed isocratic and gradient liquid-chromatography separations at 1500bar

Journal of Chromatography A

Volumn 1409, Issue , 2015, Pages 138-145

  De Vos, Jelle ^a   De Pra, Mauro ^b   Desmet, Gert ^a   Swart, Remco ^b   Edge, Tony ^b   Steiner, Frank ^b   Eeltink, Sebastiaan ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b Thermo Fisher Scientific   (United States)

Author keywords

Accucore; Kinetic gain factor; Peak compression; Poppe plot; Sub 2 m particles; UHPLC

Indexed keywords

EFFICIENCY; HIGH PRESSURE EFFECTS; LIQUID CHROMATOGRAPHY; PARTICLE SIZE; SEPARATION; SHELLS (STRUCTURES); WATER POLLUTION;

ACCUCORE; GAIN FACTORS; PEAK COMPRESSION; POPPE PLOT; UHPLC;

PARTICLE SIZE ANALYSIS;

ANALYTICAL PARAMETERS; ARTICLE; CHEMICAL REACTION KINETICS; CORE SHELL PARTICLE; GRADIENT LIQUID CHROMATOGRAPHY; GRADIENT STEEPNESS; ISOCRATIC LIQUID CHROMATOGRAPHY; KINETIC TIME GAIN FACTOR; PARTICLE SIZE; PRESSURE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REDUCED PLATE HEIGHT; SEPARATION EFFICIENCY; SEPARATION IMPEDANCE; TIME; TUBING CONFIGURATION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; WASTE WATER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; PROCEDURES;

BENZENE DERIVATIVE; KETONE;

BENZENE DERIVATIVES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; KETONES; KINETICS; PARTICLE SIZE; PRESSURE; TIME FACTORS;

EID: 84938743712     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2015.07.043     Document Type: Article

References (23)

1. Wren S.A.C., Tchelitcheff P. Use of ultra-performance liquid chromatography in pharmaceutical development. J. Chromatogr. A 2006, 1119:140-146.
2. Núnez O., Gallart-Ayalaa H., Martins C.P.B., Lucci P. New trends in fast liquid chromatography for food and environmental analysis. J. Chromatogr. A 2012, 1228:298-323.
3. Fekete S., Schappler J., Veuthey J.-L., Guillarme D. Current and future trends in UHPLC. Trends Anal. Chem. 2014, 63:2-13.
4. Fekete S., Oláh E., Fekete J. Fast liquid chromatography: the domination of core-shell and very fine particles. J. Chromatogr. A 2012, 1228:57-71.
5. González-Ruiz V., Olives A.I., Martín M.A. Core-shell particles lead the way to renewing high-performance liquid chromatography. Trends Anal. Chem. 2015, 64:17-28.
6. Hayes R., Ahmeda A., Edge T., Zhanga H. Core-shell particles: preparation, fundamentals and applications in high performance liquid chromatography. J. Chromatogr. A 2014, 1357:36-52.
7. Gritti F., Guiochon G. Perspectives on the evolution of the column efficiency in liquid chromatography. Anal. Chem. 2013, 85:3017-3035.
8. Bruns S., Grinias J.P., Blue L.E., Jorgenson J.W., Tallarek U. Morphology and separation efficiency of low-aspect-ratio capillary ultrahigh pressure liquid chromatography columns. Anal. Chem. 2012, 84:4496-4503.
9. Giddings J.C. Comparison of theoretical limit of separating speed in gas and liquid chromatography. Anal. Chem. 1965, 37:60-63.
10. Poppe H. Some reflections on speed and efficiency of modern chromatographic methods. J. Chromatogr. A 1997, 778:3-21.
11. Desmet G., Clicq D., Gzil P. Geometry-independent plate height representation methods for the direct comparison of the kinetic performance of LC supports with a different size or morphology. Anal. Chem. 2005, 77:4058-4070.
12. Eeltink S., Gzil P., Kok W.Th., Schoenmakers P.J., Desmet G. Selection of comparison criteria and experimental conditions to evaluate the kinetic performance of monolithic and packed-bed columns. J. Chromatogr. A 2006, 1130:108-114.
13. Guillarme D., Grata E., Glauser G., Wolfender J.-L., Veuthey J.-L., Rudaz S. Some solutions to obtain very efficient separations in isocratic and gradient modes using small particles size and ultra-high pressure. J. Chromatogr. A 2009, 1216:3232-3243.
14. Clicq D., Heinisch S., Rocca J.L., Cabooter D., Gzil P., Desmet G. Use of the kinetic plot method to analyze commercial high-temperature liquid chromatography systems II. Practically constrained performance comparison. J. Chromatogr. A 2007, 1146:193-201.
15. Eeltink S., Decrop W.M.C., Steiner F., Ursem M., Cabooter D., Desmet G., Kok W.Th. Use of kinetic plots for the optimization of the separation time in ultra-high-pressure LC. J. Sep. Sci. 2010, 33:2629-2635.
16. Broeckhoven K., Desmet G. The future of UHPLC: towards higher pressure and/or smaller particles?. Trends Anal. Chem. 2014, 63:6575.
17. Gritti F., Guiochon G. Mass transfer mechanism in liquid chromatography columns packed with shell particles: would there be an optimum shell structure?. J. Chromatogr. A 2010, 1217:8167-8180.
18. Liekens A., Denayer J., Desmet G. Experimental investigation of the difference in B-term dominated band broadening between fully porous and porous-shell particles for liquid chromatography using the Effective Medium Theory. J. Chromatogr. A 2011, 1218:4406-4416.
19. Broeckhoven K., Cabooter D., Eeltink S., Desmet G. Kinetic plot based comparison of the efficiency and peak capacity of high-performance liquid chromatography columns: theoretical background and selected samples. J. Chromatogr. A 2012, 1228:20-30.
20. Billen J., Broeckhoven K., Liekens A., Choikhet K., Rozing G., Desmet G. Influence of pressure and temperature on the physico-chemical properties of mobile-phase mixtures commonly used in high-performance liquid chromatography. J. Chromatogr. A 2010, 1210:30-44.
21. Neue U.D. Theory of peak capacity in gradient elution. J. Chromatogr. A 2005, 1079:153-161.
22. Vaast A., De Vos J., Broeckhoven K., Verstraeten M., Eeltink S., Desmet G. Maximizing the peak capacity using coupled columns packed with 2.6μm core-shell particles operated at 1200bar. J. Chromatogr. A 2012, 1256:72-79.
23. Snyder L.R., Dolan J.W. High-performance Gradient Elution - the Practical Application of the Linear-solvent-strength Model 2007, Wiley, Hoboken, NJ.