Identification and analysis of exosomes secreted from macrophages extracted by different methods

International Journal of Clinical and Experimental Pathology

Volumn 8, Issue 6, 2015, Pages 6135-6142

  Wang, Jianjun ^a   Yao, Yongliang ^a   Wu, Jianhong ^a   Li, Guangxin ^b  

^a Affiliated to Jiangsu University   (China)

^b Chongqing Cancer Institute   (China)

Author keywords

Exosomes; Macrophage; MHC I; TNF alpha;

Indexed keywords

BIOLOGICAL MARKER; CD68 ANTIGEN, HUMAN; CULTURE MEDIUM; DIFFERENTIATION ANTIGEN; HEAT SHOCK PROTEIN 70; LAMP1 PROTEIN, HUMAN; LEUKOCYTE ANTIGEN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN; TUMOR NECROSIS FACTOR;

COMPARATIVE STUDY; CULTURE MEDIUM; DENSITY GRADIENT CENTRIFUGATION; EXOSOME; FILTRATION; FLOW CYTOMETRY; HUMAN; MACROPHAGE; METABOLISM; PROCEDURES; SECRETION (PROCESS); TRANSMISSION ELECTRON MICROSCOPY; TUMOR CELL LINE; ULTRASTRUCTURE; WESTERN BLOTTING;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BIOMARKERS; BLOTTING, WESTERN; CELL LINE, TUMOR; CENTRIFUGATION, DENSITY GRADIENT; CULTURE MEDIA; EXOSOMES; FILTRATION; FLOW CYTOMETRY; HSP70 HEAT-SHOCK PROTEINS; HUMANS; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEINS; MACROPHAGES; MICROSCOPY, ELECTRON, TRANSMISSION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84938599885     PISSN: None     EISSN: 19362625     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Schorey JS, Bhatnagar S. Exosome function: from tumor immunology to pathogen biology. Traffic 2008; 9: 871-81.
2. Vlassov AV, Magdaleno S, Setterquist R, Conrad R. Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochim Biophys Acta 2012; 1820: 940-48.
3. Segura E, Guérin C, Hogg N, Amigorena S, Théry C. CD8+ dendritic cells use LFA-1 to capture MHC-peptide complexes from exosomes in vivo. J Immunol 2007; 179: 1489-96.
4. Laulagnier K, Motta C, Hamdi S, Roy S, Fauvelle F, Pageaux JF, Kobayashi T, Salles JP, Perret B, Bonnerot C, Record M. Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization. Biochem J 2004; 380: 161-71.
5. Woodworth JS, Wu Y, Behar SM. Mycobacterium tuberculosis-specific CD8+ T Cells require perforin to kill target cells and provide protection in vivo. J Immunol 2008; 181: 8595-603.
6. Zech D, Rana S, Büchler MW, Zöller M. Tumor-exosomes and leukocyte activation: an ambivalent crosstalk. Cell Commun Signal 2012; 10: 37.
7. Keller S, Ridinger J, Rupp AK, Janssen JW, Altevogt P. Body fluid derived exosomes as a novel template for clinical diagnostics. J Transl Med 2011; 9: 86.
8. Torregrosa Paredes P, Esser J, Admyre C, Nord M, Rahman QK, Lukic A, Rådmark O, Grönneberg R, Grunewald J, Eklund A, Scheynius A, Gabrielsson S. Bronchoalveolar lavage fluid exosomes contribute to cytokine and leukotriene production in allergic asthma. Allergy 2012; 67: 911-19.
9. Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN. Proteomic analysis of exosomes isolated from human malignant pleural effusions. Am J Respir Cell Mol Biol 2004; 31: 114-21.
10. Segura E, Guérin C, Hogg N, Amigorena S, Théry C. CD8+ dendritic cells use LFA-1 to capture MHC-peptide complexes from exosomes in vivo. J Immunol 2007; 179: 1489-1496.
11. Zitvogel L, Regnault A, Lozier A, Wolfers J, Flament C, Tenza D, Ricciardi-Castagnoli P, Raposo G, Amigorena S. Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes. Nat Med 1998; 4: 594-600.
12. Hosseini HM, Fooladi AA, Nourani MR, Ghanezadeh F. The role of exosomes in infectious diseases. Inflamm Allergy Drug Targets 2013; 12: 29-37.
13. Giri PK, Kruh NA, Dobos KM, Schorey JS. Proteomic analysis identifies highly antigenic proteins on exosomes from M. tuberculosis-infected and culture filtrate protein-treated macrophages. Proteomics 2010; 10: 3190-202.
14. O'Neill SK, Cao Y, Hamel KM, Doodes PD, Hutas G, Finnegan A. Expression of CD80/86 on B cells is essential for autoreactive T cell activation and the development of arthritis. J Immunol 2007; 179: 5109-16.
15. Gutiérrez-Vázquez C, Villarroya-Beltri C, Mittelbrunn M, Sánchez-Madrid F. Transfer of extracellular vesicles during immune cell-cell interactions. Immunol Rev 2013; 251: 125-42.
16. Chaput N, Taïeb J, Schartz N, Flament C, Novault S, André F, Zitvogel L. The potential of exosomes in immunotherapy of cancer. Blood Cells Mol Dis 2005; 35: 111-5.
17. Johnstone RM, Adam M, Hammond JR, Orr L, Turbide C. Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). J Biol Chem 1987; 262: 9412-20.
18. Tang LJ, De Seta F, Odreman F, Venge P, Piva C, Guaschino S, Garcia RC. Proteomic analysis of human cervical-vaginal fluids. J Proteome Res 2007; 6: 2874-83.
19. Qu JL, Qu XJ, Zhao MF, Teng YE, Zhang Y, Hou KZ, Jiang YH, Yang XH, Liu YP. Gastric cancer exosomes promote tumour cell proliferation through PI3K/Akt and MAPK/ERK activation. J Dig Liver Dis 2009; 41: 875-80.
20. Taylor DD, Zacharias W, Gercel-Taylor C. Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol 2011; 728: 235-46.
21. Griffiths RE, Heesom KJ, Anstee DJ. Normal prion protein trafficking in cultured humanerythroblasts. Blood 2007; 110: 4518-25.
22. Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Möbius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W. Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. J Biol Chem 2003; 278: 10963-72.
23. Yang C, Chalasani G, Ng YH, Robbins PD. Exosomes released from Mycoplasma infected tumor cells activate inhibitory B cells. PLoS One 2012; 7: e36138.
24. Qazi KR, Torregrosa Paredes P, Dahlberg B, Grunewald J, Eklund A, Gabrielsson S. Proinfl ammatory exosomes in bronchoalveolar lavage fl uid of patients with sarcoidosis. Thorax 2010; 65: 1016-24.
25. Steinman RM. Lasker Basic Medical Research Award. Dendritic cells: versatile controllers of the immune system. Nat Med 2007; 13: 1155-9.
26. Demangel C, Britton WJ. Interaction of dendritic cells with mycobacteria: where the action starts. Immunol Cell Biol 2000; 78: 318-24.
27. Lande R, Giacomini E, Grassi T, Remoli ME, Iona E, Miettinen M, Julkunen I, Coccia EM. IFN-alpha beta released by Mycobacterium tuberculosis-infected human dendritic cells induces the expression of CXCL10: selective recruitment of NK and activated T cells. J Immunol 2003; 170: 1174-82.
28. Lanzavecchia A, Sallusto F. Regulation of T cell immunity by dendritic cells. Cell 2001; 106: 263-66.