Comparative analysis of urinary total proteins by bicinchoninic acid and pyrogallol red molybdate methods

Journal of Clinical and Diagnostic Research

Volumn 9, Issue 8, 2015, Pages BC01-BC04

  Yalamati, Padma ^a   Bhongir, Aparna Varma ^a   Karra, Madhulatha ^a   Beedu, Sashidhar Rao ^b  

^a Mediciti Institute of Medical Sciences   (India)

^b OSMANIA UNIVERSITY   (India)

Author keywords

Bland and altman analysis; Correlation; Passing and bablok regression analysis; Urinary protein assays

Indexed keywords

ARTICLE; BICINCHONINIC ACID METHOD; CLINICAL ARTICLE; CROSS-SECTIONAL STUDY; HUMAN; MEASUREMENT ACCURACY; MEASUREMENT PRECISION; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN URINE LEVEL; PYROGALLOL RED MOLYBDATE METHOD; ULTRAVIOLET SPECTROPHOTOMETRY; URINALYSIS;

EID: 84938571300     PISSN: 2249782X     EISSN: 0973709X     Source Type: Journal    
DOI: 10.7860/JCDR/2015/13543.6313     Document Type: Article

References (27)

1. Savory J, Pu PH, Sunderman FWJ. A biuret method for determination of protein in normal urine. Clin Chem. 1968;14:1160–71.
2. Doetsch K, Gadsden RH. Determination of total urinary protein, combining Lowry sensitivity and biuret specificity. Clin Chem. 1973;19:1170–78.
3. Rice EW. Improved biuret procedure for routine determination of urinary total proteins in clinical proteinuria. Clin Chem. 1975;21:398–401.
4. Shahangian S, Brown PI, Ash KO. Turbidimetric measurement of total urinary proteins: A revised method. Am J Clin Pathol. 1984;81:651–54.
5. Henry RJ, Sobel C SM. Turbidimetric determination of proteins with sulphosalicylic and trichloroacetic acids. Proc Soc Exp Biol Med. 1956;92:748–51.
6. Iwata J, Nishikaze O. New micro-turbidimetric method for determination of protein in cerebrospinal fluid and urine. Clin Chem. 1979;25:1317–19.
7. McIntosh JC. Application of dye-binding method to the determination of protein in urine and cerebrospinal fluid. Clin Chem. 1977;23:1939–40.
8. Schleicher E, Wieland OH. Evaluation of the Bradford method for protein determination in body fluids. J Clin Chem Clin Biochem. 1978;16:533–34.
9. Watanabe N, Kamei S, Ohkubo A, Yamanaka M, Ohsawa S, Makino K, et al. Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyser. Clin Chem. 1986;32:1551–54.
10. McElderry LA, Tarbit IF, Cassells-Smith AJ. Six methods for urinary protein compared. Clin Chem. 1982;28:356–60.
11. Dilena BA, Penberthy LA, Fraser CG. Six methods for determining urinary protein compared. Clin Chem. 1983;29:553–57.
12. Nishi HH, Elin RJ. Three turbidimetric methods for determining total protein compared. Clin Chem. 1985;31:1377–80.
13. Pesce MA, Strande CS. A new micromethod for determination of protein in cerebrospinal fluid and urine. Clin Chem. 1973;19:1265–67.
14. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein dye binding. Anal Biochem. 1976;72:248–54.
15. Pierce BCA protein assay kit [Internet]. [cited 2014 Jan 15]. Available from: www.thermo.com/pierce. Data accessed: Instructions for BCA protein assay.
16. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985;150:76–85.
17. Shishido K, Sudo J. Reevaluation of analytical procedures on quantitation of urinary protein in rats. J Toxicol Sci. 1988;13:1–6.
18. Mistry HD, Bramham K, Weston AJ, Ward MA, Thompson AJ, Chappell LC. Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension. 2013;3:211–14.
19. Kawakami H, Murakami T, Kajii T. Normal values for 24-h urinary protein excretion: total and low molecular weight proteins with a sex-related difference. Clin Nephrol. 1990;33:232–36.
20. Altman D, Bland J. Measurement in medicine: the analysis of method comparison studies. Stat [Internet]. 1983;32:307–17. Available from: http://www.jstor.org/stable/2987937. Data accessed: proposed method of analysis.
21. Brown RE, Jarvis KL, Hyland KJ. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal Biochem. 1989;180:136–39.
22. Johnson AM, Rohlfs EM SL. Analysis of Proteins. In: Burtis CA AE, editor. Tietz Fundamentals of Clinical Chemistry. 5th edition. Philadelphia: W.B Saunders Company; 2001. pp. 343–51.
23. Johnson AM, Rohlfs EM SL. Analysis of Proteins. In: Burtis CA AE, editor. Tietz Fundamentals of Clinical Chemistry. 3rd edition. Philadelphia: W.B Saunders Company; 1999. pp. 477–540.
24. NCCLS EP5-T2: precision performance of clinical chemistry devices-Second edition- Tentative Guideline [Internet]. Wayne, Pennsylvania; 1992. Available from: https://www.lifescan.com/sites/default/files/pdf/hospital/tb867r.pdf. Data accessed: precision and accuracy.
25. Westgard JO, Carey RN, Wold S. Criteria for judging precision and accuracy in method development and evaluation. Clin Chem. 1974;20:825–33.
26. Passing H, Bablok W. A new biometrical procedure for testing the equality of measuremments from two different analytical methods. Application of linear regression procedures for method comparison studies in Clinical Chemistry, Part 1. J Clin Chem Clin Biochem. 1983;21:709–20.
27. Lowry OH, Rosenbourgh NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265–75.