Low pH increases the yield of exosome isolation

Biochemical and Biophysical Research Communications

Volumn 461, Issue 1, 2015, Pages 76-79

  Ban, Jae Jun ^a   Lee, Mijung ^b   Im, Wooseok ^b   Kim, Manho ^b,c  

^a Sejong University   (South Korea)

^b Seoul National University Hospital   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

Acidic microenvironment; Exosome; pH

Indexed keywords

CULTURE MEDIUM;

ANIMAL; BOVINE; CELL FRACTIONATION; CHEMISTRY; CULTURE MEDIUM; CULTURE TECHNIQUE; EXOSOME; HEK293 CELL LINE; HUMAN; PH; PROCEDURES; ULTRASTRUCTURE;

ANIMALS; CATTLE; CELL CULTURE TECHNIQUES; CELL FRACTIONATION; CULTURE MEDIA; EXOSOMES; HEK293 CELLS; HUMANS; HYDROGEN-ION CONCENTRATION;

EID: 84937511785     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2015.03.172     Document Type: Article

References (24)

1. A. Lakkaraju, and E. Rodriguez-Boulan Itinerant exosomes: emerging roles in cell and tissue polarity Trends Cell Biol. 18 2008 199 209
2. C. Thery, S. Amigorena, G. Raposo, and A. Clayton Isolation and characterization of exosomes from cell culture supernatants and biological fluids Curr. Protoc. Cell Biol. 2006 Chapter 3 Unit 3.22
3. S.K. Gupta, C. Bang, and T. Thum Circulating microRNAs as biomarkers and potential paracrine mediators of cardiovascular disease Circ. Cardiovasc. Genet. 3 2010 484 488
4. M. Tsujiura, D. Ichikawa, S. Komatsu, A. Shiozaki, H. Takeshita, T. Kosuga, H. Konishi, R. Morimura, K. Deguchi, H. Fujiwara, K. Okamoto, and E. Otsuji Circulating microRNAs in plasma of patients with gastric cancers Br. J. Cancer 102 2010 1174 1179
5. H. Zhou, P.S. Yuen, T. Pisitkun, P.A. Gonzales, H. Yasuda, J.W. Dear, P. Gross, M.A. Knepper, and R.A. Star Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery Kidney Int. 69 2006 1471 1476
6. A. Michael, S.D. Bajracharya, P.S. Yuen, H. Zhou, R.A. Star, G.G. Illei, and I. Alevizos Exosomes from human saliva as a source of microRNA biomarkers Oral Dis. 16 2010 34 38
7. D.M. Pegtel, K. Cosmopoulos, D.A. Thorley-Lawson, M.A. van Eijndhoven, E.S. Hopmans, J.L. Lindenberg, T.D. de Gruijl, T. Wurdinger, and J.M. Middeldorp Functional delivery of viral miRNAs via exosomes Proc. Natl. Acad. Sci. U. S. A. 107 2010 6328 6333
8. H. Valadi, K. Ekstrom, A. Bossios, M. Sjostrand, J.J. Lee, and J.O. Lotvall Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells Nat. Cell. Biol. 9 2007 654 659
9. I.S. Zeelenberg, M. Ostrowski, S. Krumeich, A. Bobrie, C. Jancic, A. Boissonnas, A. Delcayre, J.B. Le Pecq, B. Combadiere, S. Amigorena, and C. Thery Targeting tumor antigens to secreted membrane vesicles in vivo induces efficient antitumor immune responses Cancer Res. 68 2008 1228 1235
10. F. Arslan, R.C. Lai, M.B. Smeets, L. Akeroyd, A. Choo, E.N. Aguor, L. Timmers, H.V. van Rijen, P.A. Doevendans, G. Pasterkamp, S.K. Lim, and D.P. de Kleijn Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury Stem cell Res. 10 2013 301 312
11. M.C. Deregibus, V. Cantaluppi, R. Calogero, M. Lo Iacono, C. Tetta, L. Biancone, S. Bruno, B. Bussolati, and G. Camussi Endothelial progenitor cell derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA Blood 110 2007 2440 2448
12. T. Katsuda, R. Tsuchiya, N. Kosaka, Y. Yoshioka, K. Takagaki, K. Oki, F. Takeshita, Y. Sakai, M. Kuroda, and T. Ochiya Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes Sci. Reports 3 2013 1197
13. S. Sahoo, E. Klychko, T. Thorne, S. Misener, K.M. Schultz, M. Millay, A. Ito, T. Liu, C. Kamide, H. Agrawal, H. Perlman, G. Qin, R. Kishore, and D.W. Losordo Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity Circ. Res. 109 2011 724 728
14. S. Ohno, M. Takanashi, K. Sudo, S. Ueda, A. Ishikawa, N. Matsuyama, K. Fujita, T. Mizutani, T. Ohgi, T. Ochiya, N. Gotoh, and M. Kuroda Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells Mol. Ther. J. Am. Soc. Gene Ther. 21 2013 185 191
15. Y. Shao, M. Ye, X. Jiang, W. Sun, X. Ding, Z. Liu, G. Ye, X. Zhang, B. Xiao, and J. Guo Gastric juice long noncoding RNA used as a tumor marker for screening gastric cancer Cancer 120 2014 3320 3328
16. V. Estrella, T. Chen, M. Lloyd, J. Wojtkowiak, H.H. Cornnell, A. Ibrahim-Hashim, K. Bailey, Y. Balagurunathan, J.M. Rothberg, B.F. Sloane, J. Johnson, R.A. Gatenby, and R.J. Gillies Acidity generated by the tumor microenvironment drives local invasion Cancer Res. 73 2013 1524 1535
17. A. Riemann, B. Schneider, A. Ihling, M. Nowak, C. Sauvant, O. Thews, and M. Gekle Acidic environment leads to ROS-induced MAPK signaling in cancer cells PloS One 6 2011 e22445
18. I.F. Tannock, and D. Rotin Acid pH in tumors and its potential for therapeutic exploitation Cancer Res. 49 1989 4373 4384
19. I. Parolini, C. Federici, C. Raggi, L. Lugini, S. Palleschi, A. De Milito, C. Coscia, E. Iessi, M. Logozzi, A. Molinari, M. Colone, M. Tatti, M. Sargiacomo, and S. Fais Microenvironmental pH is a key factor for exosome traffic in tumor cells J. Biol. Chem. 284 2009 34211 34222
20. G. Taraboletti, S. D'Ascenzo, I. Giusti, D. Marchetti, P. Borsotti, D. Millimaggi, R. Giavazzi, A. Pavan, and V. Dolo Bioavailability of VEGF in tumor-shed vesicles depends on vesicle burst induced by acidic pH Neoplasia 8 2006 96 103
21. B. Fevrier, D. Vilette, F. Archer, D. Loew, W. Faigle, M. Vidal, H. Laude, and G. Raposo Cells release prions in association with exosomes Proc. Natl. Acad. Sci. U. S. A. 101 2004 9683 9688
22. M.L. DeMarco, and V. Daggett Molecular mechanism for low pH triggered misfolding of the human prion protein Biochemistry 46 2007 3045 3054
23. Z. Brownlee, K.D. Lynn, P.E. Thorpe, and A.J. Schroit A novel "salting-out" procedure for the isolation of tumor-derived exosomes J. Immunol. Methods 407 2014 120 126
24. S. Saman, W. Kim, M. Raya, Y. Visnick, S. Miro, S. Saman, B. Jackson, A.C. McKee, V.E. Alvarez, N.C. Lee, and G.F. Hall Exosome-associated tau is secreted in tauopathy models and is selectively phosphorylated in cerebrospinal fluid in early Alzheimer disease J. Biol. Chem. 287 2012 3842 3849