EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells

International Endodontic Journal

Volumn 49, Issue 6, 2016, Pages 581-590

  Galler, K M ^a   Widbiller, M ^a   Buchalla, W ^a   Eidt, A ^a   Hiller, K A ^a   Hoffer, P C ^a   Schmalz, G ^a,b  

^a UNIVERSITY HOSPITAL REGENSBURG   (Germany)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Dental pulp stem cells; Dentine; EDTA; Growth factors; Regenerative endodontics

Indexed keywords

EDETIC ACID;

BONE MINERALIZATION; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL MOTION; CELL SURVIVAL; CYTOLOGY; DENTIN; DRUG EFFECTS; HUMAN; PHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; STEM CELL; TOOTH PULP;

CALCIFICATION, PHYSIOLOGIC; CELL ADHESION; CELL DIFFERENTIATION; CELL MOVEMENT; CELL SURVIVAL; CELLS, CULTURED; DENTAL PULP; DENTIN; EDETIC ACID; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; STEM CELLS;

EID: 84937484645     PISSN: 01432885     EISSN: 13652591     Source Type: Journal    
DOI: 10.1111/iej.12492     Document Type: Article

References (35)

1. Arai T, Busby W, Clemmons DR (1996) Binding of insulin-like growth factor (IGF) I or II to IGF-binding protein-2 enables it to bind to heparin and extracellular matrix. Endocrinology 137, 4571-5.
2. Baker SM, Sugars RV, Wendel M et al. (2009) TGF-beta/extracellular matrix interactions in dentin matrix: a role in regulating sequestration and protection of bioactivity. Calcified Tissue International 85, 66-74.
3. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008) Growth factors and cytokines in wound healing. Wound Repair and Regeneration 16, 585-601.
4. Casagrande L, Demarco FF, Zhang Z, Araujo FB, Shi S, Nör JE (2010) Dentin-derived BMP-2 and odontoblast differentiation. Journal of Dental Research 89, 603-8.
5. Cassidy N, Fahey M, Prime SS, Smith AJ (1997) Comparative analysis of transforming growth factor-beta isoforms 1-3 in human and rabbit dentine matrices. Archives of Oral Biology 42, 219-23.
6. Czonstkowsky M, Wilson EG, Holstein FA (1990) The smear layer in endodontics. Dental Clinics of North America 34, 13-25.
7. Diogenes AR, Ruparel NB, Teixeira FB, Hargreaves KM (2014) Translational science in disinfection for regenerative endodontics. Journal of Endodontics 40, 52-7.
8. Dreyfuss JL, Regatieri CV, Jarrouge TR, Cavalheiro RP, Sampaio LO, Nader HB (2009) Heparan sulfate proteoglycans: structure, protein interactions and cell signaling. Anais Da Academia Brasileira De Ciências 81, 409-29.
9. Dung SZ, Gregory RL, Li Y, Stookey GK (1995) Effect of lactic acid and proteolytic enzymes on the release of organic matrix components from human root dentin. Caries Research 29, 483-9.
10. Ferracane JL, Cooper PR, Smith AJ (2013) Dentin matrix component solubilization by solutions of pH relevant to self-etching dental adhesives. The Journal of Adhesive Dentistry 15, 407-12.
11. Finkelman RD, Mohan S, Jennings JC, Taylor AK, Jepsen S, Baylink DJ (1990) Quantitation of growth factors IGF-I, SGF/IGF-II, and TGF-beta in human dentin. Journal of Bone and Mineral Research 5, 717-23.
12. Galler KM, Schweikl H, Thonemann B, D'Souza RN, Schmalz G (2006) Human pulp-derived cells immortalized with Simian Virus 40 T-antigen. European Journal of Oral Sciences 114, 138-46.
13. Galler KM, D'Souza RN, Federlin M et al. (2011) Dentin conditioning codetermines cell fate in regenerative endodontics. Journal of Endodontics 37, 1536-41.
14. Galler KM, Buchalla W, Hiller K-A et al. (2015) Influence of root canal disinfectants on growth factor release from dentin. Journal of Endodontics 41, 363-8.
15. Graham LW, Cooper PR, Cassidy N, Nör JE, Sloan AJ, Smith AJ (2006) The effect of calcium hydroxide on solubilisation of bio-active dentine matrix components. Biomaterials 27, 2865-73.
16. Hargreaves KM, Diogenes AR, Teixeira FB (2013) Treatment options: biological basis of regenerative endodontic procedures. Journal of Endodontics 39, 30-43.
17. He H, Yu J, Liu Y et al. (2008) Effects of FGF2 and TGFbeta1 on the differentiation of human dental pulp stem cells in vitro. Cell Biology International 32, 827-34.
18. Kalyva M, Papadimitriou S, Tziafas D (2010) Transdentinal stimulation of tertiary dentine formation and intratubular mineralization by growth factors. International Endodontic Journal 43, 382-92.
19. Lovelace TW, Henry MA, Hargreaves KM, Diogenes AR (2011) Evaluation of the delivery of mesenchymal stem cells into the root canal space of necrotic immature teeth after clinical regenerative endodontic procedure. Journal of Endodontics 37, 133-8.
20. Melin M, Joffre-Romeas A, Farges JC, Couble ML, Magloire H, Bleicher F (2000) Effects of TGFbeta1 on dental pulp cells in cultured human tooth slices. Journal of Dental Research 79, 1689-96.
21. Miller RG (2011) Simultaneous Statistical Inference. New York, USA: Springer.
22. Mullane EM, Dong Z, Sedgley CM et al. (2008) Effects of VEGF and FGF2 on the revascularization of severed human dental pulps. Journal of Dental Research 87, 1144-8.
23. Musson DS, McLachlan JL, Sloan AJ, Smith AJ, Cooper PR (2010) Adrenomedullin is expressed during rodent dental tissue development and promotes cell growth and mineralization. Biology of the Cell 102, 145-57.
24. Pang N-S, Lee SJ, Kim E et al. (2014) Effect of EDTA on attachment and differentiation of dental pulp stem cells. Journal of Endodontics 40, 811-7.
25. Paralkar VM, Vukicevic S, Reddi AH (1991) Transforming growth factor beta type 1 binds to collagen IV of basement membrane matrix: implications for development. Developmental Biology 143, 303-8.
26. Rahman S, Patel Y, Murray J et al. (2005) Novel hepatocyte growth factor (HGF) binding domains on fibronectin and vitronectin coordinate a distinct and amplified Met-integrin induced signalling pathway in endothelial cells. BMC Cell Biology 6, 8.
27. Roberts-Clark DJ, Smith AJ (2000) Angiogenic growth factors in human dentine matrix. Archives of Oral Biology 45, 1013-6.
28. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3, 1103-8.
29. Smith AJ, Murray PE, Sloan AJ, Matthews JB, Zhao S (2001) Trans-dentinal stimulation of tertiary dentinogenesis. Advances in Dental Research 15, 51-4.
30. Somasundaram R, Ruehl M, Tiling N et al. (2000) Collagens serve as an extracellular store of bioactive interleukin 2. The Journal of Biological Chemistry 275, 38170-5.
31. Suzuki T, Lee CH, Chen M et al. (2011) Induced migration of dental pulp stem cells for in vivo pulp regeneration. Journal of Dental Research 90, 1013-8.
32. Tomson PL, Grover LM, Lumley PJ, Sloan AJ, Smith AJ, Cooper PR (2007) Dissolution of bio-active dentine matrix components by mineral trioxide aggregate. Journal of Dentistry 35, 636-42.
33. Trevino EG, Patwardhan AN, Henry MA et al. (2011) Effect of irrigants on the survival of human stem cells of the apical papilla in a platelet-rich plasma scaffold in human root tips. Journal of Endodontics 37, 1109-15.
34. Zhang R, Cooper PR, Gay S, Nör JE, Smith AJ (2011) Angiogenic activity of dentin matrix components. Journal of Endodontics 37, 26-30.
35. Zhao S, Sloan AJ, Murray PE, Lumley PJ, Smith AJ (2000) Ultrastructural localisation of TGF-beta exposure in dentine by chemical treatment. The Histochemical Journal 32, 489-94.