Effects of daidzein in regards to cytotoxicity in vitro, apoptosis, reactive oxygen species level, cell cycle arrest and the expression of caspase and Bcl-2 family proteins

Oncology Reports

Volumn 34, Issue 3, 2015, Pages 1115-1120

  Han, Bing Jie ^a   Li, Wei ^a   Jiang, Guang Bin ^a   Lai, Shang Hai ^a   Zhang, Cheng ^a   Zeng, Chuan Chuan ^a   Liu, Yun Jun ^a  

^a GUANGDONG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Cytotoxicity in vitro; Daidzein; Mitochondrial membrane potential; Reactive oxygen species; Western blot analysis

Indexed keywords

BIM PROTEIN; CASPASE; DAIDZEIN; PROTEIN BCL 2; PROTEIN BCL X; REACTIVE OXYGEN METABOLITE; ISOFLAVONE DERIVATIVE; PHYTOESTROGEN;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL LINE; CELL CYCLE ARREST; CONTROLLED STUDY; DOWN REGULATION; DRUG CYTOTOXICITY; G2 PHASE CELL CYCLE CHECKPOINT; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL CARCINOMA; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; PROTEIN EXPRESSION; UPREGULATION; BIOSYNTHESIS; CELL CYCLE CHECKPOINT; CELL PROLIFERATION; COMET ASSAY; DRUG EFFECTS; FLOW CYTOMETRY; METABOLISM; TUMOR CELL LINE; WESTERN BLOTTING;

APOPTOSIS; BLOTTING, WESTERN; CASPASES; CELL CYCLE CHECKPOINTS; CELL LINE, TUMOR; CELL PROLIFERATION; COMET ASSAY; FLOW CYTOMETRY; HUMANS; IN VITRO TECHNIQUES; ISOFLAVONES; MEMBRANE POTENTIAL, MITOCHONDRIAL; PHYTOESTROGENS; PROTO-ONCOGENE PROTEINS C-BCL-2; REACTIVE OXYGEN SPECIES;

EID: 84937200227     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2015.4133     Document Type: Article

References (12)

1. Shu XO, Zheng Y, Cai H, Gu K, Chen Z, Zheng W and Lu W: Soy food intake and breast cancer survival. JAMA 302: 2437-2443, 2009.
2. Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127: 2893-2917, 2010.
3. Kreijkamp-Kaspers S, Kok L, Grobbee DE, de Haan EH, Aleman A, Lampe JW and van der Schouw YT: Effect of soy protein containing isoflavones on cognitive function, bone mineral density, and plasma lipids in postmenopausal women: A randomized controlled trial. JAMA 292: 65-74, 2004.
4. Park D, Huang T and Frishman WH: Phytoestrogens as cardioprotective agents. Cardiol Rev 13: 13-17, 2005.
5. Vedavanam K, Srijayanta S, O'Reilly J, Raman A and Wiseman H: Antioxidant action and potential antidiabetic properties of an isoflavonoid-containing soyabean phytochemical extract (SPE). Phytother Res 13: 601-608, 1999.
6. Magee PJ, Allsopp P, Samaletdin A and Rowland IR: Daidzein, R-(+)equol and S-(-)equol inhibit the invasion of MDA-MB-231 breast cancer cells potentially via the down-regulation of matrix metalloproteinase-2. Eur J Nutr 53: 345-350, 2014.
7. Hirota K, Morikawa K, Hanada H, Nonaka M, Nakajima Y, Kobayashi M and Nakajima R: Effect of genistein and daidzein on the proliferation and differentiation of human preadipocyte cell line. J Agric Food Chem 58: 5821-5827, 2010.
8. Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63, 1983.
9. Lo KK, Lee TK, Lau JS, Poon WL and Cheng SH: Luminescent biological probes derived from ruthenium(II) estradiol polypyridine complexes. Inorg Chem 47: 200-208, 2008.
10. Hickman JA: Apoptosis induced by anticancer drugs. Cancer Metastasis Rev 11: 121-139, 1992.
11. Alapetite C, Wachter T, Sage E and Moustacchi E: Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays. Int J Radiat Biol 69: 359-369, 1996.
12. Danial NN and Korsmeyer SJ: Cell death: Critical control points. Cell 116: 205-219, 2004.