Isolation, purification and in vitro differentiation of cytotrophoblast cells from human term placenta

Reproductive Biology and Endocrinology

Volumn 13, Issue 1, 2015, Pages

  Li, Liping ^a,b   Schust, Danny J ^a  

^a University of Missouri   (United States)

^b GUANGZHOU FIRST PEOPLE'S HOSPITAL   (China)

Author keywords

Cytotrophoblast cells; Differentiation; Fusion; Isolation; Placenta; Syncytiotrophoblast

Indexed keywords

CD163 ANTIGEN; CD31 ANTIGEN; CD45 ANTIGEN; CHORIONIC GONADOTROPIN BETA SUBUNIT; CYTOKERATIN 7; HLA G ANTIGEN; PEPTIDES AND PROTEINS; PROTEIN GCM 1; SYNCYTIN; UNCLASSIFIED DRUG; UVOMORULIN; VIMENTIN; CADHERIN; CHORIONIC GONADOTROPIN; GCM1 PROTEIN, HUMAN; NUCLEAR PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; CELL AGGREGATION; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CELL SELECTION; CELL VIABILITY; CONTROLLED STUDY; CYTOTROPHOBLAST; CYTOTROPHOBLAST CELL; DENSITY GRADIENT CENTRIFUGATION; ENDOTHELIUM CELL; ENZYMATIC DEGRADATION; FEMALE; FLOW CYTOMETRY; HUMAN; IN VITRO STUDY; LEUKOCYTE; MESENCHYME CELL; MONONUCLEAR CELL; NONHUMAN; PLACENTA; PROTEIN EXPRESSION; PURIFICATION; SYNCYTIOTROPHOBLAST; TROPHOBLAST; CELL SEPARATION; CYTOLOGY; GIANT CELL; METABOLISM; PHYSIOLOGY; PREGNANCY;

CADHERINS; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; CHORIONIC GONADOTROPIN; FEMALE; FLOW CYTOMETRY; GIANT CELLS; HUMANS; IN VITRO TECHNIQUES; KERATIN-7; NUCLEAR PROTEINS; PLACENTA; PREGNANCY; TRANSCRIPTION FACTORS; TROPHOBLASTS;

EID: 84936760409     PISSN: None     EISSN: 14777827     Source Type: Journal    
DOI: 10.1186/s12958-015-0070-8     Document Type: Article

References (32)

1. + channels modulate human placental trophoblast syncytialization. PLoS One. 2014;9:e90961.
2. Kliman HJ, Segel L. The placenta may predict the baby. J Theor Biol. 2003;225:143-5.
3. Bloxam DL, Bax BE, Bax CM. Culture of syncytiotrophoblast for the study of human placental transfer. Part II: Production, culture and use of syncytiotrophoblast. Placenta. 1997;18:99-108.
4. Zhuang XW, Li J, Brost BC, Xia XY, Chen HB, Wang CX, et al. Decreased expression and altered methylation of syncytin-1 gene in human placentas associated with preeclampsia. Curr Pharm Des. 2014;20:1796-802.
5. Anton L, Brown AG, Bartolomei MS, Elovitz MA. Differential methylation of genes associated with cell adhesion in preeclamptic placentas. PLoS One. 2014;9:e100148.
6. Ruebner M, Strissel PL, Langbein M, Fahlbusch F, Wachter DL, Faschingbauer F, et al. Impaired cell fusion and differentiation in placentae from patients with intrauterine growth restriction correlate with reduced levels of HERV envelope genes. J Mol Med (Berl). 2010;88:1143-56.
7. Weiss G, Goldsmith LT, Taylor RN, Bellet D, Taylor HS. Inflammation in reproductive disorders. Reprod Sci. 2009;16:216-29.
8. Hiden U, Ghaffari-Tabrizi N, Gauster M, Tam-Amersdorfer C, Cetin I, Dieber-Rotheneder M, et al. Membrane-type matrix metalloproteinase 1 regulates trophoblast functions and is reduced in fetal growth restriction. Am J Pathol. 2013;182:1563-71.
9. Kliman HJ, Nestler JE, Sermasi E, Sanger JM, Strauss 3rd JF. Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology. 1986;118:1567-82.
10. Williams SF, Fik E, Zamudio S, Illsley NP. Global protein synthesis in human trophoblast is resistant to inhibition by hypoxia. Placenta. 2012;33:31-8.
11. Díaz-Luján C, Triquell MF, Schijman A, Paglini P, Fretes RE. Differential susceptibility of isolated human trophoblasts to infection by Trypanosoma cruzi. Placenta. 2012;33:264-70.
12. Kaplan A, Chung K, Kocak H, Bertolotto C, Uh A, Hobel CJ, et al. Group B streptococcus induces trophoblast death. Microb Pathog. 2008;45:231-5.
13. Douglas GC, King BF. Isolation of pure villous cytotrophoblast from term human placenta using immunomagnetic microspheres. J Immunol Methods. 1989;119:259-68.
14. Linton EA, Rodriguez-Linares B, Rashid-Doubell F, Ferguson DJ, Redman CW. Caveolae and caveolin-1 in human term villous trophoblast. Placenta. 2003;24:745-57.
15. Sun LZ, Yang NN, De W, Xiao YS. Proteomic analysis of proteins differentially expressed in preeclamptic trophoblasts. Gynecol Obstet Invest. 2007;64:17-23.
16. Stenqvist AC, Chen T, Hedlund M, Dimova T, Nagaeva O, Kjellberg L, et al. An efficient optimized method for isolation of villous trophoblast cells from human early pregnancy placenta suitable for functional and molecular studies. Am J Reprod Immunol. 2008;60:33-42.
17. Khan S, Katabuchi H, Araki M, Nishimura R, Okamura H. Human villous macrophage-conditioned media enhance human trophoblast growth and differentiation in vitro. Biol Reprod. 2000;62:1075-83.
18. + NK with trophoblast cells during normal pregnancy and recurrent spontaneous abortion at early term of gestation. Scand J Immunol. 2014;80:198-208.
19. Singh AT, Dharmarajan A, Aye IL, Keelan JA. Sphingosine-sphingosine-1-phosphate pathway regulates trophoblast differentiation and syncytialization. Reprod Biomed Online. 2012;24:224-34.
20. Pötgens AJ, Kataoka H, Ferstl S, Frank HG, Kaufmann P. A positive immunoselection method to isolate villous cytotrophoblast cells from first trimester and term placenta to high purity. Placenta. 2003;24:412-23.
21. Sengupta J, Khan MA, Huppertz B, Ghosh D. In-vitro effects of the antimicrobial peptide Ala8,13,18-magainin II amide on isolated human first trimester villous trophoblast cells. Reprod Biol Endocrinol. 2011;9:49.
22. Salomon C, Kobayashi M, Ashman K, Sobrevia L, Mitchell MD, Rice GE. Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes. PLoS One. 2013;8:e79636.
23. Tang Z, Tadesse S, Norwitz E, Mor G, Abrahams VM, Guller S. Isolation of hofbauer cells from human term placentas with high yield and purity. Am J Reprod Immunol. 2011;66:336-48.
24. Maldonado-Estrada J, Menu E, Roques P, Barré-Sinoussi F, Chaouat G. Evaluation of Cytokeratin 7 as an accurate intracellular marker with which to assess the purity of human placental villous trophoblast cells by flow cytometry. J Immunol Methods. 2004;286:21-34.
25. Cervar-Zivkovic M, Stern C. Trophoblast isolation and culture. In: Kay HH, Nelson DM, Wang Y, editors. The placenta: from development to disease. England: Wiley-Blackwell; 2011. p. 155-62.
26. Handschuh K, Guibourdenche J, Cocquebert M, Tsatsaris V, Vidaud M, Evain-Brion D, et al. Expression and regulation by PPARγ of hCG aα- and β-subunits: comparison between villous and invasive extravillous trophoblastic cells. Placenta. 2009;30:1016-22.
27. Huppertz B, Bartz C, Kokozidou M. Trophoblast fusion: fusogenic proteins, syncytins and ADAMs, and other prerequisites for syncytial fusion. Micron. 2006;37:509-17.
28. Schmon B, Hartmann M, Jones CJ, Desoye G. Insulin and glucose do not affect the glycogen content in isolated and cultured trophoblast cells of human term placenta. J Clin Endocrinol Metab. 1991;73:888-93.
29. 5. Mol Hum Reprod. 1999;5:162-7.
30. Boyd AW. Human leukocyte antigens: an update on structure, function and nomenclature. Pathology. 1987;19:329-37.
31. Blaschitz A, Weiss U, Dohr G, Desoye G. Antibody reaction patterns in first trimester placenta: implications for trophoblast isolation and purity screening. Placenta. 2000;21:733-41.
32. Gonen-Gross T, Goldman-Wohl D, Huppertz B, Lankry D, Greenfield C, Natanson-Yaron S, et al. Inhibitory NK receptor recognition of HLA-G: regulation by contact residues and by cell specific expression at the fetal-maternal interface. PLoS One. 2010;5:e8941.