Detecting mycoplasma contamination in cell cultures by polymerase chain reaction

Methods in Molecular Biology

Volumn 731, Issue , 2011, Pages 93-103

  Uphoff, Cord C ^a   Drexler, Hans G ^a  

^a NONE

Author keywords

Cell lines; Contamination; Detection; Mycoplasma; PCR; Speciation

Indexed keywords

DNA FRAGMENT; TAQ POLYMERASE; BACTERIAL DNA; PRIMER DNA;

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; CELL CULTURE; CONTINUOUS CULTURE; DNA EXTRACTION; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; FREEZE THAWING; MYCOPLASMA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; CELL LINE; GENETICS; ISOLATION AND PURIFICATION; LABORATORY; METHODOLOGY; MICROBIOLOGY; QUALITY CONTROL; STANDARD; ANIMAL CELL CULTURE; ARTIFACT; BACTERIUM CULTURE; CONTAMINATION; GENE AMPLIFICATION; HUMAN; HUMAN CELL; RELIABILITY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENTIATION;

ANIMALIA; MYCOPLASMA; MYCOPLASMATALES;

CELL LINE; CELLS, CULTURED; DNA PRIMERS; DNA, BACTERIAL; LABORATORIES; MYCOPLASMA; POLYMERASE CHAIN REACTION; QUALITY CONTROL;

EID: 84934444634     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-80-5_8     Document Type: Article

References (5)

1. Drexler, H. G. and Uphoff, C. C. (2002) Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention Cytotechnol 39, 23-38.
2. Uphoff, C. C. and Drexler, H. G. (2001) Prevention of mycoplasma contamination in leukemia-lymphoma cell lines Human Cell 14, 244-7.
3. Hopert, A., Uphoff, C. C., Wirth, M., Hauser, H., and Drexler, H. G. (1993) Specifcity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell lines J Immunol Methods 164, 91-100.
4. Uphoff, C. C. and Drexler, H. G. (2002) Detection of mycoplasma in leukemia-lymphoma cell lines using polymerase chain reaction Leukemia 16, 289-93.
5. Uphoff, C. C. and Drexler, H. G. (2010) Contamination of cell cultures, mycoplasma. In: Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology (Flickinger M. C., ed), Wiley-Blackwell, New York, pp. 3611-3630.