Purification and characterization of human and mouse complement C3

Methods in Molecular Biology

Volumn 1100, Issue , 2014, Pages 75-91

  Ruseva, Marieta M ^a   Heurich, Meike ^b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b CARDIFF UNIVERSITY SCHOOL OF MEDICINE   (United Kingdom)

Author keywords

C3; Complement system; Hemolysis; Ion exchange chromatography

Indexed keywords

COMPLEMENT COMPONENT C3; COMPLEMENT COMPONENT C3B; PLASMINOGEN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BLOOD SAMPLING; COMPLEMENT HEMOLYSIS TEST; ERYTHROCYTE; GEL FILTRATION; HUMAN; ION EXCHANGE CHROMATOGRAPHY; MALE; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

ANIMALS; CHROMATOGRAPHY, LIQUID; COMPLEMENT C3; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEMOLYSIS; HUMANS; MICE;

EID: 84934444461     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-724-2_6     Document Type: Article

References (30)

1. de Bruijn MH, Fey GH (1985) Human complement component C3: cDNA coding sequence and derived primary structure. Proc Natl Acad Sci U S A 82:708-712
2. Lundwall A, Wetsel RA, Domdey H et al (1984) Structure of murine complement component C3. I. Nucleotide sequence of cloned complementary and genomic DNA coding for the beta chain. J Biol Chem 259:13851-13856
3. Wetsel RA, Lundwall A, Davidson F et al (1984) Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain. J Biol Chem 259:13857-13862
4. Wetsel R (1995) Structure, function and cellular expression of complement anaphylatoxin receptors. Curr Opin Immunol 7:48-53
5. Nonaka M, Natsuume-Sakai S, Kaidoh T et al (1980) Characterization of allotypic determinants of murine complement C3. J Immunol 125:2025-2030
6. Pangburn MK, Schreiber RD, Müller-Eberhard HJ (1981) Formation of the initial C3 convertase of the alternative complement pathway. Acquisition of C3b-like activities by spontaneous hydrolysis of the putative thioester in native C3. J Exp Med 154:856-867
7. Amara U, Rittirsch D, Flierl M et al (2008) Interaction between the coagulation and complement system. Adv Exp Med Biol 632:71-79
8. Brodbeck W, Mold C, Atkinson J et al (2000) Cooperation between decay-accelerating factor and membrane cofactor protein in protecting cells from autologous complement attack. J Immunol 165:3999-4006
9. Lublin DM, Atkinson JP (1989) Decay-accelerating factor: biochemistry, molecular biology, and function. Annu Rev Immunol 7:35-58
10. Nicholson-Weller A, Wang C (1994) Structure and function of decay accelerating factor CD55. J Lab Clin Med 123:485-491
11. Nicholson-Weller A, Burge J, Fearon DT et al (1982) Isolation of a human erythrocyte membrane glycoprotein with decay-accelerating activity for C3 convertases of the complement system. J Immunol 129:184-189
12. Pangburn MK, Schreiber RD, Muller-Eberhard HJ (1977) Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution. J Exp Med 146:257-270
13. Weiler JM, Daha MR, Austen KF et al (1976) Control of the amplification convertase of complement by the plasma protein beta1H. Proc Natl Acad Sci U S A 73:3268-3272
14. Morgan BP, Harris CL (1999) Complement regulatory proteins. Academic, San Diego, London
15. Fearon D (1980) Identification of the membrane glycoprotein that is the C3b receptor of the human erythrocyte, polymorphonuclear leukocyte, B lymphocyte, and monocyte. J Exp Med 152:20-30
16. Ross G, Medof M (1985) Membrane complement receptors specific for bound fragments of C3. Adv Immunol 37:217-267
17. Ross G, Lambris J, Cain J et al (1982) Generation of three different fragments of bound C3 with purified factor I or serum. I. Requirements for factor H vs. CR1 cofactor activity. J Immunol 129:2051-2060
18. Foley S, Li B, Dehoff M et al (1993) Mouse Crry/p65 is a regulator of the alternative pathway of complement activation. Eur J Immunol 23:1381-1384
19. Kim YU, Kinoshita T, Molina H et al (1995) Mouse complement regulatory protein Crry/p65 uses the specific mechanisms of both human decay-accelerating factor and membrane cofactor protein. J Exp Med 181:151-159
20. van Lookeren Campagne M, Wiesmann C, Brown EJ (2007) Macrophage complement receptors and pathogen clearance. Cell Microbiol 9:2095-2102
21. Harrison R (1996) Purification, assay, characterization of complement proteins from plasma in handbook of experimental immunology. In: Leonore A. Herzenberg (ed) Cell surface and messenger molecules of the immune system, Donald M. Weir/co-editors, vol 2, 5th edn, Caroline Blackwell. edn, Blackwell Science, Cambridge
22. Koch C, Skjødt K, Laursen I (1985) A simple immunoblotting method after separation of proteins in agarose gel. J Immunol Methods 84:271-278
23. Natsuume-Sakai S, Moriwaki K, Amano S et al (1979) Allotypes of C3 in laboratory and wild mouse distinguished by alloantisera. J Immunol 123:216-221
24. Ruseva MM, Hughes TR, Donev RM et al (2009) Crry deficiency in complement sufficient mice: C3 consumption occurs without associated renal injury. Mol Immunol 46:803-811
25. Morgan BP (2000) Complement methods and protocols. Humana, New York
26. van Dijk H, Rademaker PM, Willers JM (1980) Estimation of classical pathway of mouse complement activity by use of sensitized rabbit erythrocytes. J Immunol Methods 39: 257-268
27. Morgan BP (2000) Measurement of complement hemolytic activity, generation of complement-depleted sera, and production of hemolytic intermediates. Methods Mol Biol 150:61-71
28. Heurich M, Martínez-Barricarte R, Francis NJ et al (2011) Common polymorphisms in C3, factor B, and factor H collaborate to determine systemic complement activity and disease risk. Proc Natl Acad Sci U S A 108:8761-8766
29. Isenman DE, Kells DI, Cooper NR et al (1981) Nucleophilic modification of human complement protein C3: correlation of conformational changes with acquisition of C3b-like functional properties. Biochemistry 20:4458-4467
30. Lachmann PJ (2010) Preparing serum for functional complement assays. J Immunol Methods 352:195-197