Combined bidimensional electrophoresis and electron microscopy to study specific plasmid DNA replication intermediates in human cells

Methods in Molecular Biology

Volumn 1094, Issue , 2014, Pages 209-219

  Follonier, Cindy ^a   Lopes, Massimo ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

2D gel; DNA replication; Electron microscopy; In vivo replication intermediates; Replication fork structure; SV40

Indexed keywords

AGAROSE; GENOMIC DNA; PLASMID DNA;

ARTICLE; COMBINED BIDIMENSIONAL ELECTROPHORESIS; DNA REPLICATION; DNA REPLICATION ORIGIN; ELECTRON MICROSCOPY; ELECTROPHORESIS; HUMAN; HUMAN CELL; MOLECULAR BIOLOGY; PLASMID; PRIORITY JOURNAL;

CELL LINE; DNA REPLICATION; ELECTROPHORESIS; HUMANS; MICROSCOPY, ELECTRON; PLASMIDS; SIMIAN VIRUS 40; TRANSFECTION;

EID: 84934444346     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-706-8_16     Document Type: Article

References (4)

1. Fanning E, Zhao K (2009) SV40 DNA replication: from the A gene to a nanomachine. Virology 384:352-359
2. Follonier C, Oehler J, Herrador R, Lopes M (2013) Friedreich's Ataxia associated GAA repeats induce replication fork reversal and unusual molecular junctions in human cells. Nat Struct Mol Biol 20:486-494
3. Hirt B (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26:365-369
4. Neelsen KJ et al (2013) Visualization and interpretation of eukaryotic DNA replication intermediates In Vivo by Electron Microscopy. Methods Mol Biol 1094:177-208