Gametic cell adhesion and fusion in the unicellular alga Chlamydomonas

Methods in Molecular Biology

Volumn 475, Issue , 2008, Pages 39-51

  Wilson, Nedra F ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Cell fusion; Chlamydomonas; Fertilization tubule; Flagella; Gamete; Signal transduction

Indexed keywords

ALGAE; CHLAMYDOMONAS; EUKARYOTA;

EID: 84934444047     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-250-2_3     Document Type: Review

References (43)

1. Lien, T. and Knutsen, G. (1979) Synchronous growth of Chlamydomonas reinhardtii (Chlorophyceae): a review of optimal conditions. J. Phycol. 15, 191-200.
2. Sager, R. and Granick, S. (1954) Nutritional control of sexuality in Chlamydomonas reinhardi. J. Gen. Physiol. 37, 729-742.
3. Pan, J-M, Haring, M. A., and Beck, C. F. (1996) Dissection of the blue-light dependent signal-transduction pathway involved in gametic differentiation of Chlamydomonas reinhardtii. Plant Physiol. 112, 303-309.
4. Adair, W. S., Monk, B. C., Cohen, R., Hwang, C., and Goodenough, U. W. (1982) Sexual agglutinins from the Chlamydomonas flagellar membrane. Partial purification and characterization. J. Biol. Chem. 257, 4593-4602.
5. Adair, W. S., Hwang, C., and Goodenough, U. W. (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33, 183-193.
6. Cooper, J. B., Adair, W. S., Mecham, R. P., Heuser, J. E., and Goodenough, U. W. (1983) Chlamydomonas agglutinin is a hydroxyproline-rich glycoprotein. Proc. Natl. Acad. Sci. U.S.A. 80, 5898-5901.
7. Collin-Osdoby, P., and Adair, W. S. (1985) Characterization of the purified Chlamydomonas minus agglutinin. J. Cell Biol. 101, 1144-1152.
8. Ferris, P. J., Waffenschmidt, S., Umen, J. G., Lin, H., Lee, J-H., Ishida, K., Kubo, T., Lau, J., and Goodenough, U. W. (2005) Plus and minus sexual agglutinins from Chlamydomonas reinhardtii. Plant Cell 17, 597-615.
9. Goodenough, U. W. and Jurivich, D. (1978) Tipping and mating-structure activation induced in Chlamydomonas gametes by flagellar membrane antisera. J. Cell Biol. 79, 680-693.
10. Goodenough, U. W. (1993) Tipping of flagellar agglutinins by gametes of Chlamydomonas reinhardtii. Cell Motil. Cytoskeleton 25, 179-189.
11. Snell, W. J. and Roseman, S. (1979) Kinetics of adhesion and de-adhesion of Chlamydomonas gametes. J. Biol. Chem. 254, 10820-10829.
12. Hunnicutt, G. R., Kosfiszer, M. G., and Snell, W. J. (1990) Cell body and flagellar agglutinins in Chlamydomonas reinhardtii: the cell body plasma membrane is a reservoir for agglutinins whose migration to the flagella is regulated by a functional barrier. J. Cell Biol. 111, 1605-1616.
13. Goodenough, U. W. (1989) Cyclic AMP enhances the sexual agglutinability of Chlamydomonas flagella. J. Cell Biol. 109, 247-252.
14. Pasquale, S. M. and Goodenough, U. W. (1987) Cyclic AMP functions as a primary sexual signal in gametes of Chlamydomonas reinhardtii. J. Cell Biol. 105, 2279-2292.
15. Saito, T., Small, L., and Goodenough, U. W. (1993) Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and heat. J. Cell Biol. 122, 137-147.
16. Zhang, Y., Ross, E. M., and Snell, W. J. (1991) ATP-dependent regulation of flagellar adenylylcyclase in gametes of Chlamydomonas reinhardtii. J. Biol. Chem. 266, 22954-22959.
17. Zhang, Y. and Snell, W. J. (1993) Differential regulation of adenylylcyclases in vegetative and gametic flagella of Chlamydomonas. J. Biol. Chem. 268, 1786-1791.
18. Zhang, Y. and Snell, W. J. (1994) Flagellar adhesion-dependent regulation of Chlamydomonas adenylyl cyclase in vitro: a possible role for protein kinases during sexual signaling. J. Cell Biol. 125, 617-624.
19. Wang, Q. and Snell, W. J. (2003) Flagellar adhesion between mating type plus and mating type minus gametes activates a flagellar protein-tyrosine kinase during fertilization in Chlamydomonas. J. Biol. Chem. 278, 32936-32942.
20. Wang, Q., Pan, J., and Snell, W. J. (2006) Intraflagellar transport particles participate directly in cilium-generated signaling in Chlamydomonas. Cell 125, 549-562.
21. Kozminski, K. G., Johnson, K. A., Forscher, P., and Rosenbaum, J. L. (1993) A motility in the eukaryotic flagellum unrelated to flagellar beating. Proc. Natl. Acad. Sci. U.S.A. 90, 5519-5523.
22. Qin, H., Diener, D. R., Geimer, S., Cole, D. G., and Rosenbaum, J. L. (2004) Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body. J. Cell Biol. 164, 255-266.
23. Singla, V. and Reiter, J. F. (2006) The primary cilium as the cell's antenna: signaling at a sensory organelle. Science 313, 629-633.
24. Piperno, G., Mead, K., and Henderson, S. (1996) Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1 (FLA10) to reach the distal part of flagella in Chlamydomonas. J. Cell Biol. 133, 371-379.
25. Pan, J. and Snell, W. J. (2002) Kinesin-II is required for flagellar sensory transduction during fertilization in Chlamydomonas. Mol. Biol. Cell 13, 1417-1426.
26. Imam, S. H. and Snell, W. J. (1988) The Chlamydomonas cell wall degrading enzyme, lysin, acts on two substrates within the framework of the wall. J. Cell Biol. 106, 2211-2221.
27. Matsuda, Y., Saito, T., Yamaguchi, T., and Kawase, H. (1985) Cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii is a metalloprotease and digests the sodium perchlorate-insoluble component of cell wall. J. Biol. Chem. 260, 6373-6377.
28. Matsuda, Y., Saito, T., Yamaguchi, T., Koseki, M., and Hayashi, K. (1987) Topography of cell wall lytic enzyme in Chlamydomonas reinhardtii: form and location of the stormed enzyme in vegetative cell and gamete. J. Cell Biol. 104, 321-329.
29. Buchanan, M. J., Imam, S. H., Eskue, W. A., and Snell, W. J. (1989) Activation of the cell wall degrading protease, lysin, during sexual signalling in Chlamydomonas. The enzyme is stored as an inactive, higher relative molecular mass precursor in the periplasm. J. Cell Biol. 108, 199-207.
30. Snell, W. J., Eskue, W. A., and Buchanan, M. J. (1989) Regulated secretion of a serine protease that activates an extracellular matrix-degrading metalloprotease during fertilization in Chlamydomonas. J. Cell Biol. 109, 1689-1694.
31. Goodenough, U. W. and Weiss, R. L. (1975) Gametic differentiation in Chlamydomonas reinhardtii III. Cell wall lysis and microfilament- associated mating structure activation in wild-type and mutant strains. J. Cell Biol. 67, 623-637.
32. Goodenough, U. W., Detmers, P. A., and Hwang, C. (1982) Activation for cell fusion in Chlamydomonas: analysis of wild-type gametes and nonfusing mutants. J. Cell Biol. 92, 378-386.
33. Detmers, P. A., Goodenough, U. W., and Condeelis, J. (1983) Elongation of the fertilization tubule in Chlamydomonas: new observations on the core microfilaments and the effect of transient intracellular signals on their structural integrity. J. Cell Biol. 97, 522-532.
34. Detmers, P. A., Carboni, J. M., and Condeelis, J. (1985) Localization of actin in Chlamydomonas using antiactin and NBD-phallacidin. Cell Motil. 5, 415-430.
35. Goodenough, U. W. and Weiss, R. L. (1978) Interrelationships between microtubules, a striated fiber, and the gametic mating structures of Chlamydomonas reinhardtii. J. Cell Biol. 76, 430-438.
36. Forest, C. L. (1983) Specific contact between mating structure membranes observed in conditional fusion-defective Chlamydomonas mutants. Exp. Cell Res. 148, 143-154.
37. Weiss, R. L., Goodenough, D. A., and Goodenough, U. W. (1977) Membrane differentiations at sites specialized for cell fusion. J. Cell Biol. 72, 144-160.
38. Wilson, N. F., Foglesong, M. J., and Snell, W. J. (1997) The Chlamydomonas mating type plus fertilization tubule, a prototypic cell fusion organelle: isolation, characterization, and in vitro adhesion to mating type minus gametes. J. Cell Biol. 30, 1537-1553.
39. Wilson, N. F. (1996) The Chlamydomonas mt+ fertilization tubule: a model system for studying the role of cell fusion organelles in gametic cell fusion [Ph.D. thesis]. UT Southwestern Medical Center, Dallas.
40. Ferris, P. J., Woessner, J. P., and Goodenough, U. W. (1996) A sex recognition glycoprotein is encoded by the plus mating-type gene fus1 of Chlamydomonas reinhardtii. Mol. Biol. Cell 7, 1235-1248.
41. Misamore, M. J., Gupta, S., and Snell, W. J. (2003) The Chlamydomonas Fus1 protein is present on the mating type plus fusion organelle and required for a critical membrane adhesion event during fusion with minus gametes. Mol. Biol. Cell 14, 2530-2542.
42. Vallance, B. A. and Finlay, B. B. (2000) Exploitation of host cells by enteropathogenic Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97, 8799-8806.
43. Forest, C. L. (1987) Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J. Cell Sci. 88, 613-621.