Isolation and propagation of colon cancer stem cells

Methods in Molecular Biology

Volumn 1035, Issue , 2013, Pages 247-259

  Prasetyanti, Pramudita R ^a   Zimberlin, Cheryl ^a   De Sousa E Melo, Felipe ^a   Medema, Jan Paul ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Adenoma; Cancer stem cells; CD133; Colorectal cancer; EGF; Isolation; Ultralow adherent; Wnt signaling

Indexed keywords

MATRIGEL; WNT PROTEIN;

ADHERENT CELL; ANIMAL CELL; ARTICLE; CANCER STEM CELL; CELL COUNTING; CELL DEDIFFERENTIATION; CELL DENSITY; CELL DIFFERENTIATION; CELL ISOLATION; CELL SUSPENSION; COCULTURE; COLON ADENOMA; COLON CANCER; COLORECTAL CANCER; CULTURE MEDIUM; HUMAN; HUMAN CELL; HUMAN TISSUE; LAMINAR FLOW; MOUSE; MYOFIBROBLAST; NONHUMAN; PRIORITY JOURNAL; SMALL INTESTINE; STEM CELL EXPANSION; TISSUE CULTURE; WNT SIGNALING PATHWAY;

EID: 84934443196     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-508-8_21     Document Type: Article

References (8)

1. Clarke MF, Dick JE, Dirks PB, Eaves CJ, Jamieson CH et al (2006) Cancer stem cells- perspectives on current status and future directions: AACR Workshop on cancer stem cells. Cancer Res 66:9339-9344
2. Huelsken MM, Wakamiya J, Ding J, van de Wetering M, Clevers H et al (2004) Beta- catenin regulates Cripto expression during embryogenesis and tumor formation. Eur J Cell Biol 83:31
3. Lee J, Kotliarova S, Kotliarov Y, Li A, Su Q et al (2006) Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell 9:391-403
4. O'Brien CA, Pollett A, Gallinger S, Dick JE (2007) A human colon cancer cell capable of initiating tumour growth in immunodefi cientmice. Nature 445:106-110
5. Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M et al (2007) Identifi cation and expansion of human colon-cancer- initiating cells. Nature 445:111-115
6. Todaro M, Alea MP, Di Stefano AB, Cammareri P, Vermeulen L et al (2007) Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4. Cell Stem Cell 1:389-402
7. Vermeulen L, De Sousa EM, van der Heijden M, Cameron K, de Jong JH et al (2010) Wnt activity defi nes colon cancer stem cells and is regulated by the microenvironment. Nat Cell Biol 12:468-476
8. Vermeulen L, Todaro M, de Sousa MF, Sprick MR, Kemper K et al (2008) Single-cell cloning of colon cancer stem cells reveals a multi- lineage differentiation capacity. Proc Natl Acad Sci USA 105:13427-13432