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Volumn 488, Issue , 2008, Pages 41-63

Assembly and glycerol gradient isolation of yeast spliceosomes containing transcribed or synthetic U6 snRNA

Author keywords

Glycerol gradient sedimentation; pre mRNA splicing; RNA ligation; Saccharomyces cerevisiae; SP6 transcription; spliceosome; Splicing extracts; T7 transcription; U6 reconstitution

Indexed keywords

SACCHAROMYCES CEREVISIAE;

EID: 84934443096     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-475-3_4     Document Type: Article
Times cited : (3)

References (45)
  • 1
    • 0026861506 scopus 로고
    • The RNP motif protein family
    • Haynes, S. R. (1992) The RNP motif protein family. New Biol. 4, 421-429.
    • (1992) New Biol , vol.4 , pp. 421-429
    • Haynes, S.R.1
  • 2
    • 0036948420 scopus 로고    scopus 로고
    • Allosteric cascade of spliceosome activation
    • Brow, D. A. (2002) Allosteric cascade of spliceosome activation. Annu. Rev. Genet. 36, 333-360.
    • (2002) Annu. Rev. Genet , vol.36 , pp. 333-360
    • Brow, D.A.1
  • 3
    • 0032489021 scopus 로고    scopus 로고
    • Mechanical devices of the spliceosome: Motors, clocks, springs, and things
    • Staley, J. P., and Guthrie, C. (1998) Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell 92, 315-326.
    • (1998) Cell , vol.92 , pp. 315-326
    • Staley, J.P.1    Guthrie, C.2
  • 4
    • 0021799778 scopus 로고
    • The "spliceosome": Yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction
    • Brody, E., and Abelson, J. (1985) The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. Science 228, 963-967.
    • (1985) Science , vol.228 , pp. 963-967
    • Brody, E.1    Abelson, J.2
  • 5
    • 0023296411 scopus 로고
    • Splicing of yeast nuclear premRNA in vitro requires a functional 40S spliceosome and several extrinsic factors
    • Lin, R. J., Lustig, A. J., and Abelson, J. (1987) Splicing of yeast nuclear premRNA in vitro requires a functional 40S spliceosome and several extrinsic factors. Genes Dev. 1, 7-18.
    • (1987) Genes Dev , vol.1 , pp. 7-18
    • Lin, R.J.1    Lustig, A.J.2    Abelson, J.3
  • 6
    • 0022354874 scopus 로고
    • A multicomponent complex is involved in the splicing of messenger RNA precursors
    • Grabowski, P. J., Seiler, S. R., and Sharp, P. A. (1985) A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell 42, 345-353.
    • (1985) Cell , vol.42 , pp. 345-353
    • Grabowski, P.J.1    Seiler, S.R.2    Sharp, P.A.3
  • 7
    • 0024792715 scopus 로고
    • In vitro assembly of yeast U6 snRNP: A functional assay
    • Fabrizio, P., McPheeters, D. S., and Abelson, J. (1989) In vitro assembly of yeast U6 snRNP: a functional assay. Genes Dev. 3, 2137-2150.
    • (1989) Genes Dev , vol.3 , pp. 2137-2150
    • Fabrizio, P.1    McPheeters, D.S.2    Abelson, J.3
  • 8
    • 0027145630 scopus 로고
    • Functional reconstitution of U6 snRNA in nematode cis- and trans-splicing: U6 can serve as both a branch acceptor and a 5′ exon
    • Yu, Y. T., Maroney, P. A., and Nilsen, T. W. (1993) Functional reconstitution of U6 snRNA in nematode cis- and trans-splicing: U6 can serve as both a branch acceptor and a 5′ exon. Cell 75, 1049-1059.
    • (1993) Cell , vol.75 , pp. 1049-1059
    • Yu, Y.T.1    Maroney, P.A.2    Nilsen, T.W.3
  • 9
    • 0022521854 scopus 로고
    • Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein
    • Black, D. L., and Steitz, J. A. (1986) Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein. Cell 46, 697-704.
    • (1986) Cell , vol.46 , pp. 697-704
    • Black, D.L.1    Steitz, J.A.2
  • 10
    • 0021691190 scopus 로고
    • The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors
    • Kramer, A., Keller, W., Appel, B., and Luhrmann, R. (1984) The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors. Cell 38, 299-307.
    • (1984) Cell , vol.38 , pp. 299-307
    • Kramer, A.1    Keller, W.2    Appel, B.3    Luhrmann, R.4
  • 11
    • 0024376480 scopus 로고
    • Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing
    • Winkelmann, G., Bach, M., and Luhrmann, R. (1989) Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing. EMBO J. 8, 3105-3112.
    • (1989) EMBO J , vol.8 , pp. 3105-3112
    • Winkelmann, G.1    Bach, M.2    Luhrmann, R.3
  • 12
    • 0020655280 scopus 로고
    • Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine- specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera
    • Bringmann, P., Rinke, J., Appel, B., Reuter, R., and Luhrmann, R. (1983) Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine- specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera. EMBO J. 2, 1129-1135.
    • (1983) EMBO J , vol.2 , pp. 1129-1135
    • Bringmann, P.1    Rinke, J.2    Appel, B.3    Reuter, R.4    Luhrmann, R.5
  • 13
    • 0022413541 scopus 로고
    • Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences
    • Frendewey, D., and Keller, W. (1985) Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences. Cell 42, 355-367.
    • (1985) Cell , vol.42 , pp. 355-367
    • Frendewey, D.1    Keller, W.2
  • 14
    • 0022549635 scopus 로고
    • Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs
    • Konarska, M. M., and Sharp, P. A. (1986) Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell 46, 845-855.
    • (1986) Cell , vol.46 , pp. 845-855
    • Konarska, M.M.1    Sharp, P.A.2
  • 15
    • 0032708815 scopus 로고    scopus 로고
    • Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels
    • Das, R., and Reed, R. (1999) Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels. RNA 5, 1504-1508.
    • (1999) RNA , vol.5 , pp. 1504-1508
    • Das, R.1    Reed, R.2
  • 16
    • 0037126061 scopus 로고    scopus 로고
    • Purification and electron microscopic visualization of functional human spliceosomes
    • Zhou, Z., Sim, J., Griffith, J., and Reed, R. (2002) Purification and electron microscopic visualization of functional human spliceosomes. Proc. Natl. Acad. Sci. U. S. A. 99, 12203-12207.
    • (2002) Proc. Natl. Acad. Sci. U. S. A , vol.99 , pp. 12203-12207
    • Zhou, Z.1    Sim, J.2    Griffith, J.3    Reed, R.4
  • 17
    • 0036242097 scopus 로고    scopus 로고
    • Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis
    • Jurica, M. S., Licklider, L. J., Gygi, S. R., Grigorieff, N., and Moore, M. J. (2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA 8, 426-439.
    • (2002) RNA , vol.8 , pp. 426-439
    • Jurica, M.S.1    Licklider, L.J.2    Gygi, S.R.3    Grigorieff, N.4    Moore, M.J.5
  • 18
    • 0025941556 scopus 로고
    • U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction
    • Yean, S. L., and Lin, R. J. (1991) U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction. Mol. Cell. Biol. 11, 5571-5577.
    • (1991) Mol. Cell. Biol , vol.11 , pp. 5571-5577
    • Yean, S.L.1    Lin, R.J.2
  • 19
    • 0029965552 scopus 로고    scopus 로고
    • Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing
    • Kim, S. H., and Lin, R. J. (1996) Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol. Cell. Biol. 16, 6810-6819.
    • (1996) Mol. Cell. Biol , vol.16 , pp. 6810-6819
    • Kim, S.H.1    Lin, R.J.2
  • 20
    • 0030338611 scopus 로고    scopus 로고
    • Analysis of small nuclear RNAs in a precatalytic spliceosome
    • Yean, S. L., and Lin, R. J. (1996) Analysis of small nuclear RNAs in a precatalytic spliceosome. Gene Expr. 5, 301-313.
    • (1996) Gene Expr , vol.5 , pp. 301-313
    • Yean, S.L.1    Lin, R.J.2
  • 22
    • 0025149080 scopus 로고
    • A yeast mutant, PRP20, altered in mRNA metabolism and maintenance of the nuclear structure, is defective in a gene homologous to the human gene RCC1 which is involved in the control of chromosome condensation
    • Aebi, M., Clark, M. W., Vijayraghavan, U., and Abelson, J. (1990) A yeast mutant, PRP20, altered in mRNA metabolism and maintenance of the nuclear structure, is defective in a gene homologous to the human gene RCC1 which is involved in the control of chromosome condensation. Mol. Gen. Genet. 224, 72-80.
    • (1990) Mol. Gen. Genet , vol.224 , pp. 72-80
    • Aebi, M.1    Clark, M.W.2    Vijayraghavan, U.3    Abelson, J.4
  • 23
    • 28344451166 scopus 로고    scopus 로고
    • Optimizing splinted ligation of highly structured small RNAs
    • Kurschat, W. C., Muller, J., Wombacher, R., and Helm, M. (2005) Optimizing splinted ligation of highly structured small RNAs. RNA 11, 1909-1914.
    • (2005) RNA , vol.11 , pp. 1909-1914
    • Kurschat, W.C.1    Muller, J.2    Wombacher, R.3    Helm, M.4
  • 24
    • 0034131335 scopus 로고    scopus 로고
    • Joining of RNAs by splinted ligation
    • Moore, M. J., and Query, C. C. (2000) Joining of RNAs by splinted ligation. Methods Enzymol. 317, 109-123.
    • (2000) Methods Enzymol , vol.317 , pp. 109-123
    • Moore, M.J.1    Query, C.C.2
  • 25
    • 0036676886 scopus 로고    scopus 로고
    • The conserved central domain of yeast U6 snRNA: Importance of U2-U6 helix Ia in spliceosome assembly
    • Ryan, D. E., and Abelson, J. (2002) The conserved central domain of yeast U6 snRNA: importance of U2-U6 helix Ia in spliceosome assembly. RNA 8, 997-1010.
    • (2002) RNA , vol.8 , pp. 997-1010
    • Ryan, D.E.1    Abelson, J.2
  • 26
    • 0034649666 scopus 로고    scopus 로고
    • Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome
    • Yean, S. L., Wuenschell, G., Termini, J., and Lin, R. J. (2000) Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome. Nature 408, 881-884.
    • (2000) Nature , vol.408 , pp. 881-884
    • Yean, S.L.1    Wuenschell, G.2    Termini, J.3    Lin, R.J.4
  • 28
    • 0022907583 scopus 로고
    • The yeast RNA gene products are essential for mRNA splicing in vitro
    • Lustig, A. J., Lin, R. J., and Abelson, J. (1986) The yeast RNA gene products are essential for mRNA splicing in vitro. Cell 47, 953-963.
    • (1986) Cell , vol.47 , pp. 953-963
    • Lustig, A.J.1    Lin, R.J.2    Abelson, J.3
  • 29
    • 0026340961 scopus 로고
    • How does RNase H recognize a DNA.RNA hybrid?
    • Nakamura, H., Oda, Y., Iwai, S., et al. (1991) How does RNase H recognize a DNA.RNA hybrid? Proc. Natl. Acad. Sci. U. S. A. 88, 11535-11539.
    • (1991) Proc. Natl. Acad. Sci. U. S. A , vol.88 , pp. 11535-11539
    • Nakamura, H.1    Oda, Y.2    Iwai, S.3
  • 30
    • 0023653323 scopus 로고    scopus 로고
    • Kretzner, L., Rymond, B. C., and Rosbash, M. (1987) S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA. Cell 50, 593-602.
    • Kretzner, L., Rymond, B. C., and Rosbash, M. (1987) S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA. Cell 50, 593-602.
  • 31
    • 0024788534 scopus 로고
    • In vitro reconstitution of functional yeast U2 snRNPs
    • McPheeters, D. S., Fabrizio, P., and Abelson, J. (1989) In vitro reconstitution of functional yeast U2 snRNPs. Genes Dev. 3, 2124-2136.
    • (1989) Genes Dev , vol.3 , pp. 2124-2136
    • McPheeters, D.S.1    Fabrizio, P.2    Abelson, J.3
  • 32
    • 0025001918 scopus 로고
    • Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing
    • Fabrizio, P., and Abelson, J. (1990) Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing. Science 250, 404-409.
    • (1990) Science , vol.250 , pp. 404-409
    • Fabrizio, P.1    Abelson, J.2
  • 33
    • 33748029810 scopus 로고    scopus 로고
    • Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing
    • McGrail, J. C., Tatum, E. M., and O'Keefe, R. T. (2006) Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing. EMBO J. 25, 3813-3822.
    • (2006) EMBO J , vol.25 , pp. 3813-3822
    • McGrail, J.C.1    Tatum, E.M.2    O'Keefe, R.T.3
  • 34
    • 0026654005 scopus 로고
    • Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro
    • Fabrizio, P., and Abelson, J. (1992) Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro. Nucleic Acids Res. 20, 3659-3664.
    • (1992) Nucleic Acids Res , vol.20 , pp. 3659-3664
    • Fabrizio, P.1    Abelson, J.2
  • 35
    • 33748029810 scopus 로고    scopus 로고
    • Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing
    • McGrail, J. C., Tatum, E. M., and O'Keefe, R. T. (2006) Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing. EMBO J. 25, 3813-3822.
    • (2006) EMBO J , vol.25 , pp. 3813-3822
    • McGrail, J.C.1    Tatum, E.M.2    O'Keefe, R.T.3
  • 36
    • 0029377886 scopus 로고
    • SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome
    • Roscigno, R. F., and Garcia-Blanco, M. A. (1995) SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome. RNA 1, 692-706.
    • (1995) RNA , vol.1 , pp. 692-706
    • Roscigno, R.F.1    Garcia-Blanco, M.A.2
  • 37
    • 0025777075 scopus 로고
    • Isolation of HnRNP particles from Drosophila melanogaster embryos
    • Schenkel, J. (1991) Isolation of HnRNP particles from Drosophila melanogaster embryos. Biochem. Int. 24, 423-428.
    • (1991) Biochem. Int , vol.24 , pp. 423-428
    • Schenkel, J.1
  • 38
    • 0024232371 scopus 로고
    • Electron microscopic identification of the yeast spliceosome
    • Clark, M. W., Goelz, S., and Abelson, J. (1988) Electron microscopic identification of the yeast spliceosome. EMBO J. 7, 3829-3836.
    • (1988) EMBO J , vol.7 , pp. 3829-3836
    • Clark, M.W.1    Goelz, S.2    Abelson, J.3
  • 39
    • 0021995992 scopus 로고
    • Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors
    • Konarska, M. M., Grabowski, P. J., Padgett, R. A., and Sharp, P. A. (1985) Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors. Nature 313, 552-557.
    • (1985) Nature , vol.313 , pp. 552-557
    • Konarska, M.M.1    Grabowski, P.J.2    Padgett, R.A.3    Sharp, P.A.4
  • 40
    • 0023449728 scopus 로고
    • Spliceosome assembly in yeast
    • Cheng, S. C., and Abelson, J. (1987) Spliceosome assembly in yeast. Genes Dev. 1, 1014-1027.
    • (1987) Genes Dev , vol.1 , pp. 1014-1027
    • Cheng, S.C.1    Abelson, J.2
  • 41
    • 0027364421 scopus 로고
    • Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing
    • Moore, M. J., and Sharp, P. A. (1993) Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature 365, 364-368.
    • (1993) Nature , vol.365 , pp. 364-368
    • Moore, M.J.1    Sharp, P.A.2
  • 42
    • 0029258928 scopus 로고
    • Keeping RNA happy
    • Uhlenbeck, O. C. (1995) Keeping RNA happy. RNA 1, 4-6.
    • (1995) RNA , vol.1 , pp. 4-6
    • Uhlenbeck, O.C.1
  • 43
    • 0015424833 scopus 로고
    • Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells
    • Kitamura, K., and Yamamoto, Y. (1972) Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells. Arch. Biochem. Biophys. 153, 403-406.
    • (1972) Arch. Biochem. Biophys , vol.153 , pp. 403-406
    • Kitamura, K.1    Yamamoto, Y.2
  • 44
    • 0024710729 scopus 로고    scopus 로고
    • Vijayraghavan, U., Company, M., and Abelson, J. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 3, 1206-1216.
    • Vijayraghavan, U., Company, M., and Abelson, J. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 3, 1206-1216.
  • 45
    • 0025336797 scopus 로고
    • Measurement and analysis of yeast pre-mRNA sequence contribution to splicing efficiency
    • Rymond, B. C., Pikielny, C., Seraphin, B., Legrain, P., and Rosbash, M. (1990) Measurement and analysis of yeast pre-mRNA sequence contribution to splicing efficiency. Methods Enzymol. 181, 122-147.
    • (1990) Methods Enzymol , vol.181 , pp. 122-147
    • Rymond, B.C.1    Pikielny, C.2    Seraphin, B.3    Legrain, P.4    Rosbash, M.5


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