Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties

Methods in Molecular Biology

Volumn 978, Issue , 2013, Pages 45-61

  Baxter, Sarah K ^a,b,c   Lambert, Abigail R ^b,c   Scharenberg, Andrew M ^a,b,c,d   Jarjour, Jordan ^c,d  

^a UNIVERSITY OF WASHINGTON   (United States)

^b SEATTLE CHILDREN S RESEARCH INSTITUTE   (United States)

^c Northwest Genome Engineering Consortium   (United States)

^d Pregenen Inc   (United States)

Author keywords

Binding affinity; Flow cytometry; Homing endonuclease; Meganuclease; Protein DNA interaction; Yeast surface display; Yeast transformation

Indexed keywords

DOUBLE STRANDED DNA; ENDONUCLEASE; POLYACRYLAMIDE GEL; SYNTHETIC DNA;

ARTICLE; BINDING ASSAY; CATALYSIS; CONTROLLED STUDY; DNA BINDING; DNA CLEAVAGE; DNA DETERMINATION; DNA SEQUENCE; FLOW CYTOMETRY; FUNGUS GROWTH; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SACCHAROMYCES CEREVISIAE; SURFACE PROPERTY; YEAST;

EID: 84934442376     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-293-3_4     Document Type: Article

References (16)

1. Takeuchi R, Lambert AR, Mak AN-S, Jacoby K, Dickson RJ, Gloor GB, Scharenberg AM, Edgell DR, Stoddard BL (2011) Tapping natural reservoirs of homing endonucleases for targeted gene modification. Proc Natl Acad Sci U S A 108(32): 13077-13082
2. Heath PJ, Stephens KM, Monnat RJ, Stoddard BL (1997) The structure of I-Crel, a group I intron-encoded homing endonuclease. Nat Struct Biol 4(6): 468-476
3. Duan X, Gimble FS, Quiocho FA (1997) Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity. Cell 89(4): 555-564
4. Gimble FS (2000) Invasion of a multitude of genetic niches by mobile endonuclease genes. FEMS Microbiol Lett 185(2): 99-107
5. Jurica MS, Stoddard BL (1999) Homing endonucleases: structure, function and evolution. Cell Mol Life Sci 55(10): 1304-1326
6. Thermes V, Grabher C, Ristoratore F, Bourrat F, Choulika A, Wittbrodt J, Joly JS (2002) I-SceI meganuclease mediates highly efficient transgenesis infish. Mech Dev 118(1-2): 91-98
7. Gouble A, Smith J, Bruneau S et al (2006) Efficient in toto targeted recombination in mouse liver by meganuclease-induced double-strand break. J Gene Med 8(5): 616-622
8. Arnould S, Perez C, Cabaniols JP, Smith J, Gouble A, Grizot S, Epinat JC, Duclert A, Duchateau P, Paques F (2007) Engineered I-CreI derivatives cleaving sequences from the human XPC gene can induce highly efficient gene correction in mammalian cells. J Mol Biol 371(1): 49-65
9. Gao H, Smith J, Yang M et al (2010) Heritable targeted mutagenesis in maize using a designed endonuclease. Plant J 61(1): 176-187
10. Windbichler N, Papathanos PA, Catteruccia F, Ranson H, Burt A, Crisanti A (2007) Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos. Nucleic Acids Res 35(17): 5922-5933
11. Smith F, Rouet P, Romanienko PJ, Jasin M (1995) Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells. Nucleic Acids Res 23(24): 5012-5019
12. Jarjour J, West-Foyle H, Certo MT, Hubert CG, Doyle L, Getz MM, Stoddard BL, Scharenberg AM (2009) High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display. Nucleic Acids Res 37(20): 6871-6880
13. Volná P, Jarjour J, Baxter S, Roffler SR, Monnat RJ, Stoddard BL, Scharenberg AM (2007) Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Res 35(8): 2748-2758
14. Gietz RD, Schiestl RH (2007) High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2(1): 31-34
15. Gietz RD, Schiestl RH (2007) Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2(1): 1-4
16. Gietz RD, Schiestl RH (2007) Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2(1): 38-41