In vivo parathyroid hormone treatments and RNA isolation and analysis

Methods in Molecular Biology

Volumn 455, Issue , 2008, Pages 79-87

  Xin, Li ^a   Ling, Qin ^b   Nicola, C Partridge ^b  

^a UNIVERSITY OF MICHIGAN SCHOOL OF DENTISTRY   (United States)

^b RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

Bone; In vivo; Infusion; Injection; PTH; RNA

Indexed keywords

DRUG ANALYSIS; DRUG THERAPY; PARATHYROID HORMONE; PHARMACOLOGY; PTH PROTEIN, HUMAN; RNA;

ANALYTICAL EQUIPMENT; ANIMAL; ARTICLE; BONE; DRUG EFFECT; DRUG THERAPY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOUSE; OSTEOPOROSIS; PHYSIOLOGY; RAT;

ANALYSIS; ANIMALS; BONE AND BONES; DRUG EFFECTS; DRUG THERAPY; GENETICS; HUMANS; ISOLATION AND PURIFICATION; MICE; OSTEOPOROSIS; PARATHYROID HORMONE; PHARMACOLOGY; PHYSIOLOGY; RATS; REAGENT KITS, DIAGNOSTIC; RNA; THERAPEUTIC USE;

ANIMALIA; MUS; RATTUS;

EID: 84934442256     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-104-8_5     Document Type: Article

References (10)

1. Bauer, W., Aub, J., Albright, F. (1929) Studies in calcium phosphorus metabolism: study of bone trabeculae as ready available reserve supply of calcium. J Exp Med 49, 145-162.
2. Burr, D. B., Hirano, T., Turner, C. H., et al. (2000) Intermittently administered human parathyroid hormone (1-34) treatment increases intracortical bone turnover and porosity without reducing bone strength in the humerus of ovariectomized cynomolgus monkeys. J Bone Miner Res 16, 157-165.
3. Neer, R. M., Arnaud, C. D., Zanchetta, J. R., et al. (2001) Effect of parathyroid hormone (1-34) on fractures and bone mineral density in postmenopausal women with osteoporosis. NEJM 344, 1434-1441.
4. Dempster, D. W., Cosman, F., Kurland, E. S., et al. (2001) Effects of daily treatment with parathyroid hormone on bone microarchitecture and turnover in patients with osteoporosis: a paired biopsy study. J Bone Miner Res 16, 1846-1853.
5. Brommage, R., Hotchkiss, C. E., Lees, C. J., et al. (1999) Daily treatment with human recombinant parathyroid hormone-(1-34), LY333334, for 1 year increases bone mass in ovariectomized monkeys. J Clin Endocrinol Metab 84, 3757-3763.
6. Onyia, J. E., Bidwell, J., Herring, J., et al. (1995) In vivo, human parathyroid hormone fragment (hPTH 1-34) transiently stimulates immediate early response gene expression, but not proliferation, in trabecular bone cells of young rats. Bone 17, 479-484.
7. Alzet Guide to Pump Use (Alzet).
8. Protocol for Sigma's TRI Reagent (Sigma).
9. RNase Mini Handbook (Qiagen).
10. Li, X. (2006) Parathyroid hormone's dual effects (anabolic versus catabolic) on osteoblast gene expression. Doctoral dissertation. University of Medicine and Dentistry of New Jersey, Newark, NJ.