Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses

Methods in Molecular Biology

Volumn 454, Issue , 2008, Pages 229-236

  De Haan, Cornelis A M ^a   Haijema, Bert Jan ^a   Masters, Paul S ^b   Rottier, Peter J M ^c  

^a UTRECHT UNIVERSITY   (Netherlands)

^b WADSWORTH CENTER   (United States)

^c NONE

Author keywords

Coronavirus; Feline infectious peritonitis virus; Host cell switching; Mouse hepatitis virus; Reverse genetics; Targeted RNA recombination; Tropism

Indexed keywords

CORONAVIRUS; FELIDAE; FELINE INFECTIOUS PERITONITIS VIRUS; MURINAE; MURINE HEPATITIS VIRUS;

EID: 84934442153     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-181-9_17     Document Type: Article

References (7)

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2. Koetzner, C. A., et al. (1992) Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination. J. Virol. 66, 1841-1848.
3. van der Most, R. G., et al. (1992) Homologous RNA recombination allows efficient introduction of site-specific mutations into the genome of coronavirus MHV-A59 via synthetic co-replicating RNAs. Nucleic Acids Res. 20, 3375-3381.
4. Masters, P. S., et al. (1994) Optimization of targeted RNA recombination and mapping of a novel nucleocapsid gene mutation in the coronavirus mouse hepatitis virus. J. Virol. 68, 328-337.
5. Godeke, G. J., et al. (2000) Assembly of spikes into coronavirus particles ismediated by the carboxy-terminal domain of the spike protein. J. Virol. 74, 1566-1571.
6. Kuo, L., et al. (2000) Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: crossing the host cell species barrier. J. Virol. 74, 1393-1406.
7. Haijema, B. J., Volders, H., and Rottier, P. J. (2003). Switching species tropism: an effective way to manipulate the feline coronavirus genome. J. Virol. 77, 4528-38.