Analysis of compartmentalized cAMP: A method to compare signals from differently targeted FRET reporters

Methods in Molecular Biology

Volumn 1071, Issue , 2014, Pages 59-71

  Stangherlin, Alessandra ^a   Koschinski, Andreas ^a   Terrin, Anna ^a   Zoccarato, Anna ^a   Jiang, He ^a   Fields, Laura Ashley ^a   Zaccolo, Manuela ^a,b  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

cAMP compartmentalization; F rster resonance energy transfer (FRET); Imaging; Targeted sensors

Indexed keywords

CYCLIC AMP; FORSKOLIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CALCULATION; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CHO CELLS; COLFORSIN; CRICETINAE; CRICETULUS; CYCLIC AMP; FLUORESCENCE RESONANCE ENERGY TRANSFER; GUANINE NUCLEOTIDE EXCHANGE FACTORS;

EID: 84934442042     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-622-1_5     Document Type: Article

References (20)

1. Clegg R (1996) Fluorescence resonance energy transfer. In: Wang XF, Herman B (eds) Fluorescence imaging spectroscopy and microscopy, vol 137. Wilely, New York, pp 179-251
2. Zaccolo M, De Giorgi F, Cho CY et al (2000) A genetically encoded, fluorescent indicator for cyclic AMP in living cells. Nat Cell Biol 2: 25-29
3. Zaccolo M, Pozzan T (2002) Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes. Science 295:1711-1715
4. Terrin A, Di Benedetto G, Pertegato V et al (2006) PGE(1) stimulation of HEK293 cells generates multiple contiguous domains with different [cAMP]: role of compartmentalized phosphodiesterases. J Cell Biol 175:441-451
5. Di Benedetto G, Zoccarato A, Lissandron V et al (2008) Protein kinase A type I and type II define distinct intracellular signaling compartments. Circ Res 103:836-844
6. Monterisi S, Favia M, Guerra L et al (2012) CFTR regulation in human airway epithelial cells requires integrity of the actin cytoskeleton and compartmentalized cAMP and PKA activity. J Cell Sci 125(Pt 5):1106-1117
7. DiPilato LM, Cheng X, Zhang J (2004) Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signalling within discrete subcellular compartments. Proc Natl Acad Sci U S A 101: 16513-16518
8. Sin YY, Edwards HV, Li X et al (2011) Disruption of the cyclic AMP phosphodiesterase-4 (PDE4)-HSP20 complex attenuates the beta-agonist induced hypertrophic response in cardiac myocytes. J Mol Cell Cardiol 50: 872-883
9. Herget S, Lohse MJ, Nikolaev VO (2008) Real-time monitoring of phosphodiesterase inhibition in intact cells. Cell Signal 20: 1423-1431
10. Mohamed TM, Oceandy D, Zi M et al (2011) Plasma membrane calcium pump (PMCA4)-neuronal nitric-oxide synthase complex regulates cardiac contractility through modulation of a compartmentalized cyclic nucleotide microdomain. J Biol Chem 286:41520-41529
11. Matthiesen K, Nielsen J (2011) Cyclic AMP control measured in two compartments in HEK293 cells: phosphodiesterase K(M) is more important than phosphodiesterase localization. PLoS One 6:e24392
12. Wachten S, Masada N, Ayling LJ et al (2010) Distinct pools of cAMP centre on different isoforms of adenylyl cyclase in pituitary-derived GH3B6 cells. J Cell Sci 123:95-106
13. Castro LR, Gervasi N, Guiot E et al (2010) Type 4 phosphodiesterase plays different integrating roles in different cellular domains in pyramidal cortical neurons. J Neurosci 30: 6143-6151
14. Nikolaev VO, Bunemann M, Hein L et al (2004) Novel single chain cAMP sensors for receptor-induced signal propagation. J Biol Chem 279:37215-37218
15. Gesellchen F, Stangherlin A, Surdo N et al (2011) Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. Methods Mol Biol 746: 297-316
16. Borner S, Schwede F, Schlipp A et al (2011) FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells. Nat Protoc 6:427-438
17. Berrera M, Dodoni G, Monterisi S et al (2008) A toolkit for real-time detection of cAMP: insights into compartmentalized signaling. Handb Exp Pharmacol 186:285-298
18. Evellin S, Mongillo M, Terrin A et al (2004) Measuring dynamic changes in cAMP using fluorescence resonance energy transfer. Methods Mol Biol 284:259-270
19. Zhang J, Ma Y, Taylor SS et al (2001) Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc Natl Acad Sci U S A 98:14997-15002
20. Nikolaev VO, Gambaryan S, Lohse MJ (2006) Fluorescent sensors for rapid monitoring of intracellular cGMP. Nat Methods 3:23-25