The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration

Methods in Molecular Biology

Volumn 454, Issue , 2008, Pages 103-107

  Jones, Brenda V ^a   Hennion, Ruth M ^a  

^a INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

Author keywords

Ciliostasis; Respiratory virus; Tracheal organ culture; Viral assay

Indexed keywords

EUKARYOTA;

EID: 84934440361     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-181-9_9     Document Type: Article

References (8)

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2. Tyrell, D. A. J., and Bynoe, M. L. (1965) Cultivation of novel type of common-cold virus in organ cultures. Br. Med. J. 5448, 1467-1470.
3. Cummiskey, J. F., Hallum, J. V., Skinner, M. S., and Leslie, G. A. (1973) Persistent Newcastle disease virus infection in embryonic chicken tracheal organ cultures. Infect. Immun. 8 (4), 657-664.
4. Higgins, P. G., and Ellis, E. M. (1972) The isolation of influenza viruses. J. Clin. Path. 25, 521-524.
5. Cherry, J. D., and Taylor-Robinson, D. (1970) Large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies. Appl. Microbiol. 19(4), 658-662.
6. Hodgson, T.,Casais, R., Dove, B., Britton, P. and Cavanagh, D (2004) Recombinant infectious bronchitis coronavirus Baudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity. J. Virol. 78(24), 13804-13811.
7. Cook, J. K. A., Darbyshire, J. H., and Peters, R. W. (1976) The use of chicken tracheal organ cultures for the isolation and assay of infectious bronchitis virus. Arch. Virol. 50, 109-118.
8. Bhattacharjee, P. S., Naylor C. J., and Jones R. C. (1994) A simple method for immunofluorescence staining of tracheal organ cultures for the rapid identification of infectious bronchitis virus. Avian Pathol. 23, 471-480.