Application of reverse transcription-PCR and real-time PCR in nanotoxicity research

Methods in Molecular Biology

Volumn 926, Issue , 2012, Pages 99-112

  Mo, Yiqun ^a   Wan, Rong ^a   Zhang, Qunwei ^a  

^a UNIVERSITY OF LOUISVILLE   (United States)

Author keywords

Agarose gel electrophoresis; Polymerase chain reaction (PCR); Real time fluorescent quantitative PCR; Reverse transcription (RT); RNA isolation

Indexed keywords

DNA POLYMERASE; RNA DIRECTED DNA POLYMERASE;

AQUEOUS SOLUTION; ARTICLE; DNA BASE COMPOSITION; DNA CONTAMINATION; MOLONEY LEUKEMIA ONCOVIRUS; NANOTOXICOLOGY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SECONDARY STRUCTURE; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA EXTRACTION; RNA ISOLATION;

EID: 84934439616     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-2-1_7     Document Type: Article

References (3)

1. Product information, Technical Bulletin MB-205. TRI Reagent™ (Sigma, St. Louis, MO), Cat. # T9424
2. Usage information. M-MLV reverse transcriptase (Promega, Madison, WI), Cat. #M1701
3. Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor