메뉴 건너뛰기




Volumn 978, Issue , 2013, Pages 173-186

Protein engineering: Single or multiple site-directed mutagenesis

Author keywords

Gibson assembly; Inverse PCR mutagenesis; Site directed mutagenesis; Synthetic biology

Indexed keywords

DNA POLYMERASE; NUCLEOTIDE;

EID: 84934439066     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-293-3_13     Document Type: Article
Times cited : (24)

References (14)
  • 1
    • 0019252609 scopus 로고
    • Sitespecific mutagenesis using oligodeoxyribonucleotides: Isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency
    • Gillam S, Astell CR, Smith M (1980) Sitespecific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency. Gene 12: 129-137
    • (1980) Gene , vol.12 , pp. 129-137
    • Gillam, S.1    Astell, C.R.2    Smith, M.3
  • 2
    • 0020971324 scopus 로고
    • Oligonucleotidedirected mutagenesis of DNA fragments cloned into M13 vectors
    • Zoller MJ, Smith M (1983) Oligonucleotidedirected mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol 100: 468-500
    • (1983) Methods Enzymol , vol.100 , pp. 468-500
    • Zoller, M.J.1    Smith, M.2
  • 3
    • 0022470481 scopus 로고
    • Direct cloning and sequence analysis of enzymatically amplified genomic sequences
    • Scharf SJ, Horn GT, Erlich HA (1986) Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233: 1076-1078
    • (1986) Science , vol.233 , pp. 1076-1078
    • Scharf, S.J.1    Horn, G.T.2    Erlich, H.A.3
  • 4
    • 77957219390 scopus 로고
    • A simple method for sitedirected mutagenesis using the polymerase chain reaction
    • Hemsley A, Arnheim N, Toney MD, Cortopassi G, Galas DJ (1989) A simple method for sitedirected mutagenesis using the polymerase chain reaction. Nucleic Acids Res 17: 6545-6551
    • (1989) Nucleic Acids Res , vol.17 , pp. 6545-6551
    • Hemsley, A.1    Arnheim, N.2    Toney, M.D.3    Cortopassi, G.4    Galas, D.J.5
  • 5
    • 0023684064 scopus 로고
    • A general method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interactions
    • Higuchi R, Krummel B, Saiki RK (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 16: 7351-7367
    • (1988) Nucleic Acids Res , vol.16 , pp. 7351-7367
    • Higuchi, R.1    Krummel, B.2    Saiki, R.K.3
  • 6
    • 0024520745 scopus 로고
    • Site-directed mutagenesis by overlap extension using the polymerase chain reaction
    • Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59
    • (1989) Gene , vol.77 , pp. 51-59
    • Ho, S.N.1    Hunt, H.D.2    Horton, R.M.3    Pullen, J.K.4    Pease, L.R.5
  • 7
    • 22244483744 scopus 로고    scopus 로고
    • A rapid and efficient PCRbased mutagenesis method applicable to cell physiology study
    • Ko JK, Ma J (2005) A rapid and efficient PCRbased mutagenesis method applicable to cell physiology study. Am J Physiol Cell Physiol 288:C1273-C1278
    • (2005) Am J Physiol Cell Physiol , vol.288
    • Ko, J.K.1    Ma, J.2
  • 8
    • 0025643362 scopus 로고
    • A general method for rapid site-directed mutagenesis using the polymerase chain reaction
    • Landt O, Grunert HP, Hahn U (1990) A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene 96: 125-128
    • (1990) Gene , vol.96 , pp. 125-128
    • Landt, O.1    Grunert, H.P.2    Hahn, U.3
  • 9
    • 0030743251 scopus 로고    scopus 로고
    • Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method
    • Ke SH, Madison EL (1997) Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method. Nucleic Acids Res 25: 3371-3372
    • (1997) Nucleic Acids Res , vol.25 , pp. 3371-3372
    • Ke, S.H.1    Madison, E.L.2
  • 10
    • 34250170787 scopus 로고    scopus 로고
    • Deletion mutagenesis of large areas in Plasmodium falciparum genes: A comparative study
    • Williams M, Louw AI, Birkholtz LM (2007) Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study. Malar J 6:64
    • (2007) Malar J , vol.6 , pp. 64
    • Williams, M.1    Louw, A.I.2    Birkholtz, L.M.3
  • 11
    • 4344678207 scopus 로고    scopus 로고
    • Expression improvement and mechanistic study of the retro-Diels-Alderase catalytic antibody 10F11 by site-directed mutagenesis
    • Zheng L, Goddard JP, Baumann U, Reymond JL (2004) Expression improvement and mechanistic study of the retro-Diels-Alderase catalytic antibody 10F11 by site-directed mutagenesis. J Mol Biol 341: 807-814
    • (2004) J Mol Biol , vol.341 , pp. 807-814
    • Zheng, L.1    Goddard, J.P.2    Baumann, U.3    Reymond, J.L.4
  • 12
    • 0028052614 scopus 로고
    • PCR mutagenesis and recombination in vivo
    • Jones DH (1994) PCR mutagenesis and recombination in vivo. PCR Methods Appl 3:S141-S148
    • (1994) PCR Methods Appl , vol.3
    • Jones, D.H.1
  • 13
    • 73349139923 scopus 로고    scopus 로고
    • Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
    • Gibson DG (2009) Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides. Nucleic Acids Res 37: 6984-6990
    • (2009) Nucleic Acids Res , vol.37 , pp. 6984-6990
    • Gibson, D.G.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.