Plasmid template design and in vitro transcription of short RNAs within a "structure cassette" for structure probing experiments

Methods in Molecular Biology

Volumn 941, Issue , 2012, Pages 157-169

  Vachon, Virginia K ^a   Conn, Graeme L ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

In vitro transcription; Reverse transcriptase; RNA structure probing; SHAPE; Structure cassette

Indexed keywords

POLYACRYLAMIDE GEL;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; BINDING SITE; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; IN VITRO STUDY; MOLECULE; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; RESTRICTION SITE; RNA CONFORMATION; RNA STRUCTURE; RNA TRANSCRIPTION; SITE DIRECTED MUTAGENESIS; SYNTHESIS;

EID: 84934438601     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-113-4_12     Document Type: Article

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