The use of artificial MicroRNA technology to control gene expression in Arabidopsis thaliana

Methods in Molecular Biology

Volumn 1062, Issue , 2014, Pages 211-224

  Eamens, Andrew L ^a   McHale, Marcus ^a   Waterhouse, Peter M ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

amiRNA; Arabidopsis; miRNA; Plant expression vector; RNA silencing; Target gene expression

Indexed keywords

MICRORNA;

ARABIDOPSIS THALIANA; ARTICLE; CLONING VECTOR; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; GENE SILENCING; MOLECULAR CLONING; NONHUMAN; PLANT DEVELOPMENT; PLANT GENETICS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS);

AGROBACTERIUM TUMEFACIENS; ARABIDOPSIS; BASE SEQUENCE; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, PLANT; GENETIC VECTORS; MICRORNAS; MOLECULAR SEQUENCE DATA; PLASMIDS; RNA INTERFERENCE;

EID: 84934438370     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-580-4_11     Document Type: Article

References (28)

1. Reinhart BJ et al (2002) MicroRNAs in plants. Genes Dev 16:1616-1626
2. Adenot X et al (2006) DRB4-dependent TAS3 trans -acting siRNAs control leaf morphology through AGO7. Curr Biol 16:927-932
3. Borsani O et al (2005) Endogenous siRNAs derived from a pair of natural cis -antisense transcripts regulate salt tolerance in Arabidopsis. Cell 123:1279-1291
4. Onodera Y et al (2005) Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation. Cell 120:613-622
5. Pontes O et al (2006) The Arabidopsis chromatin-modifying nuclear siRNA pathway involves a nucleolar RNA processing center. Cell 126:79-92
6. Boutet S et al (2003) Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Curr Biol 13:843-848
7. Dunoyer P et al (2004) Probing the microRNA and small interfering RNA pathways with virus-encoded suppressors of RNA silencing. Plant Cell 16:1235-1250
8. Sunkar R, Zhu JK (2004) Novel and stressregulated microRNAs and other small RNAs from Arabidopsis. Plant Cell 16:2001-2019
9. Mallory AC, Vaucheret H (2006) Functions of microRNAs and related small RNAs in plants. Nat Genet 38:S31-S36
10. Park W et al (2002) CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana. Curr Biol 12:1484-1495
11. Golden TA et al (2002) SHORT INTEGUMENTS1/SUSPENSOR1/CARPEL FACTORY, a Dicer homolog, is a maternal effect gene required for embryo development in Arabidopsis. Plant Physiol 130: 808-822
12. Gasciolli V et al (2005) Partially redundant functions of Arabidopsis DICER-like enzymes and a role for DCL4 in producing trans -acting siRNAs. Curr Biol 15:1494-1500
13. Xie Z et al (2005) DICER-LIKE4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana. Proc Natl Acad Sci U S A 102:12984-12989
14. Xie Z et al (2004) Genetic and functional diversification of small RNA pathways in plants. PLoS Biol 2:e104
15. Smith NA et al (2000) Total silencing by intron-spliced hairpin RNAs. Nature 407: 319-320
16. Stoutjesdijk PA et al (2004) hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing. Plant Physiol 129:1723-1731
17. Jackson AL, Linsley PS (2004) Noise amidst the silence: off-target effects of siRNAs? Trends Genet 20:521-524
18. Xu P et al (2006) Computational estimation and experimental verification of off-target silencing during posttranscriptional gene silencing in plants. Plant Physiol 142: 429-440
19. Senthil-Kumar M, Mysore KS (2011) Caveat of RNAi in plants: the off-target effect. Methods Mol Biol 744:13-25
20. Eamens AL et al (2009) The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes. RNA 15:2219-2235
21. Eamens AL et al (2011) Efficient silencing of endogenous microRNAs using artificial microRNAs in Arabidopsis thaliana. Mol Plant 4:157-170
22. Eamens AL, Waterhouse PM (2011) Vectors and methods for hairpin RNA and artificial microRNA-mediated gene silencing in plants. Methods Mol Biol 701:179-197
23. Parizotto EA et al (2004) In vivo investigation of the transcription, processing, endonucleolytic activity, and functional relevance of the spatial distribution of a plant miRNA. Genes Dev 18:2237-2242
24. Alvarez JP et al (2006) Endogenous and synthetic microRNAs stimulate simultaneous, effi-cient, and localized regulation of multiple targets in diverse species. Plant Cell 18:1134-1151
25. Niu QW et al (2006) Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Nat Biotechnol 24: 1420-1428
26. Schwab R et al (2006) Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18:1121-1133
27. Qu J, Ye J, Fang R (2007) Artificial miRNAmediated virus resistance in plants. J Virol 81:6690-6699
28. Mi S et al (2008) Sorting of small RNAs into Arabidopsis Argonaute complexes is directed by the 5′ terminal nucleotide. Cell 133:1-12