Study of glucose uptake in adipose cells

Methods in Molecular Biology

Volumn 456, Issue , 2008, Pages 307-315

  Shi, Jun ^a   Kandror, Konstantin V ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adipocytes; Cell differentiation; Flow cytometry; Glucose; Glut4; Insulin

Indexed keywords

MAMMALIA;

EID: 84934437702     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-245-8_23     Document Type: Article

References (10)

1. Saltiel AR and Kahn CR (2001) Insulin signalling and the regulation of glucose and lipid metabolism. Nature 414:799-806
2. Kandror KV (2003) A long search for Glut4 activation. Sci STKE 2003:PE5
3. Shi J, Kandror KV (2005) Sortilin is essential and sufficient for the formation of Glut4-storage vesicles in 3T3-L1 adipocytes. Dev Cell 9:99-108
4. Carvalho E, Schellhorn SE, Zabolotny JM, Martin S, Tozzo E, Peroni OD, Houseknecht KL, Mundt A, James DE, Kahn BB (2004) GLUT4 overexpression or deficiency in adipocytes of transgenic mice alters the composition of GLUT4 vesicles and the subcellular localization of GLUT4 and insulin-responsive aminopeptidase. J Biol Chem 279:21598-21605
5. Kanai F, Nishioka Y, Hayashi H, Kamohara S, Todaka M, Ebina Y (1993) Direct demonstration of insulin-induced GLUT4 translocation to the surface of intact cells by insertion of a cmyc epitope into an exofacial GLUT4 domain. J Biol Chem 268:14523-14526
6. Kishi K, Muromoto N, Nakaya Y, Miyata I, Hagi A, Hayashi H, Ebina Y (1998) Bradykinin directly triggers GLUT4 translocation via an insulin-independent pathway. Diabetes 47:550-558
7. Ueyama A, Yaworsky KL, Wang Q, Ebina Y, Klip A (1999) GLUT-4 myc ectopic expression in L6 myoblasts generates a GLUT-4-specific pool conferring insulin sensitivity. Am J Physiol 277:E572-E578
8. Karylowski O, Zeigerer A, Cohen A, McGraw TE (2004) GLUT4 is retained by an intracellular cycle of vesicle formation and fusion with endosomes. Mol Biol Cell 15:870-882
9. Govers R, Coster AC, James DE (2004) Insulin increases cell surface GLUT4 levels by dose dependently discharging GLUT4 into a cell surface recycling pathway. Mol Cell Biol 24:6456-6466
10. Bogan JS, McKee AE and Lodish HF (2001) Insulin-responsive compartments containing Glut4 in 3T3-L1 and CHO cells: regulation by amino acid concentrations. Mol Cell Biol 21:4785-4806