Bioluminescent imaging of MAPK function with intein-mediated reporter gene assay

Methods in Molecular Biology

Volumn 574, Issue , 2009, Pages 185-192

  Kanno, Akira ^a   Ozawa, Takeaki ^a   Umezawa, Yoshio ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

intein; Luciferase; reporter gene; transcription factor

Indexed keywords

EID: 84934437402     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-321-3_15     Document Type: Article

References (10)

1. Seger, R., and Krebs, E. G. (1995) The MAPK signaling cascade. FASEB J 9, 726-735.
2. Mor, A., and Philips, M. R. (2006) Compartmentalized Ras/MAPK signaling. Annu Rev Immunol 24, 771-800.
3. Hirata, R., Ohsumi, Y., and Anraku, Y. (1989) Functional molecular masses of vacuolar membrane H+-ATPase from Saccharomyces cerevisiae as studied by radiation inactivation analysis. FEBS Lett 244, 397-401.
4. Kane, P. M., Yamashiro, C. T., Wolczyk, D. F., Neff, N., Goebl, M., and Stevens, T. H. (1990) Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. Science 250, 651-657.
5. Noren, C. J., Wang, J. M., and Perler, F. B. (2000) Dissecting the chemistry of protein splicing and its applications. Angew Chem Int Ed 39, 451-466.
6. Paulus, H. (2000) Protein splicing and related forms of protein autoprocessing. Annu Rev Biochem 69, 447-496.
7. Cooper, A. A., Chen, Y. J., Lindorfer, M. A., and Stevens, T. H. (1993) Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision. EMBO J 12, 2575-2583.
8. Chong, S. R., and Xu, M. Q. (1997) Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs. J Biol Chem 272, 15587-15590.
9. Rhee, Y., Gurel, F., Gafni, Y., Dingwall, C., and Citovsky, V. (2000) A genetic system for detection of protein nuclear import and export. Nat Biotechnol 18, 433-437.
10. Lorenz, W. W., McCann, R. O., Longiaru, M., and Cormier, M. J. (1991) Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc Natl Acad Sci USA 88, 4438-4442.