Use of gas-phase fractionation to increase protein identifications: Application to the peroxisome

Methods in Molecular Biology

Volumn 432, Issue , 2008, Pages 217-228

  Kennedy, Jacob ^a   Yi, Eugene C ^a  

^a ZYMOGENETICS INC   (United States)

Author keywords

Data dependent duty cycle; Gas phase fractionation; Liquid chromatography tandem mass spectrometry; Mass spectrometry; Nycodenz gradient fraction; Organelles; Peroxisome; Subcellular; Yeast

Indexed keywords

EID: 84934437039     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-28-7_15     Document Type: Article

References (5)

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2. Davis, M. T., Spahr, C. S., McGinley, M. D., Robinson, J. H., Bures, E. J., Beierle, J., et al. (2001) Towards defining the urinary proteome using liquid chromatographytandem mass spectrometry II. Limitations of complex mixture analyses. Proteomics 1, 108-117.
3. Yi., E. C., Marelli, M., Lee, H., Purvine, S. O., Aebersold, R., Aitchison, J. D., et al. (2002) Approaching complete peroxisome characterization by gas-phase fractionation. Electrophoresis 23, 3205-3216.
4. Marelli, M., Smith, J. J., Jung, S., Yi, E., Nesvizhskii, A. I., Christmas, R. H., et al. Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane (2004) J. Cell Biol. 167, 1099-1112.
5. Bonifacino, J. S., Dasso, M., Harford, J. B., Lippincott-Schwartz, J., Yamada, K. M. (2001) Current Protocols in Cell Biology, Vol 1.