A cell-free expression platform for production of protein microarrays

Methods in Molecular Biology

Volumn 1118, Issue , 2014, Pages 297-307

  Zárate, Xristo ^a   Galbraith, David W ^b  

^a UNIVERSIDAD AUTÓNOMA DE NUEVO LEÓN   (Mexico)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

Cell free protein expression; Green fluorescent protein; Hybrid cell free protein expression; Protein microarrays; S30 extract; Tyramide signal amplification; Wheat germ extract

Indexed keywords

CHIMERIC PROTEIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; PROTEIN; PROTEIN TAG;

ARTICLE; CELL FREE EXPRESSION SYSTEM; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; GENE EXPRESSION SYSTEM; GENETIC TRANSCRIPTION; HYBRID; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN MICROARRAY; RNA TRANSLATION; WHEAT GERM;

CELL EXTRACTS; CELL-FREE SYSTEM; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; PROTEIN ARRAY ANALYSIS; PROTEIN BIOSYNTHESIS;

EID: 84934436713     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-782-2_21     Document Type: Article

References (7)

1. Kaushansky A, Allen JE, Gordus A et al (2010) Quantifying protein-protein interactions in high throughput using protein domain microarrays. Nat Protoc 5:773-790
2. Gong W, He K, Covington M et al (2008) The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors. Mol Plant 1:27-41
3. Ramachandran N, Hainsworth E, Bhullar B et al (2004) Self-assembling protein microarrays. Science 305:86-90 (Pubitemid 38869377)
4. Ramachandran N, Raphael JV, Hainsworth E et al (2008) Next-generation high-density selfassembling functional protein arrays. Nat Methods 5:535-538 (Pubitemid 351761761)
5. Zarate X, Henderson DC, Phillips KC et al (2010) Development of high-yield autofl uorescent protein microarrays using hybrid cellfree expression with combined Escherichia coli S30 and wheat germ extracts. Proteome Sci 8:32
6. Kimzey MJ, Zarate X, Galbraith DW et al (2011) Optimizing microarray-based in situ transcription and translation of proteins for matrix-assisted laser desorption ionization mass spectrometry. Anal Biochem 414:282-286
7. Bourzac KM, Rounseville MP, Zarate X et al (2011) A high-density quantitative nuclease protection microarray platform for high-throughput analysis of gene expression. J Biotechnol 154:68-75