Method for extraction of high-quantity and -quality cell-free DNA from amniotic fluid

Methods in Molecular Biology

Volumn 444, Issue , 2008, Pages 303-309

  Lapaire, Olav ^a,b   Johnson, Kirby L ^a   Bianchi, Diana W ^a  

^a TUFTS MEDICAL CENTER   (United States)

^b UNIVERSITY WOMEN S HOSPITAL   (Switzerland)

Author keywords

Cell free fetal DNA; Pregnancy; Prenatal diagnosis; Prenatal screening

Indexed keywords

EID: 84934436537     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-66-9_24     Document Type: Article

References (7)

1. Lo, Y. M. D, Corbetta, N., Chamberlain, P. F., et al. (1997) Presence of fetal DNA in maternal plasma and serum. Lancet 350, 485-487.
2. Zhong, X. Y., Laivuori, H., Livingston, J. C., et al. (2001) Elevation of both maternal and fetal extracellular circulating deoxyribonucleic acid concentrations in the plasma of pregnant women with preeclampsia. Am. J. Obstet. Gynecol. 184, 414-419.
3. Wataganara, T., LeShane, E.S., Farina, A., et al. (2003) Maternal serum cell-free fetal DNA levels are increased in cases of trisomy 13, but not trisomy 18. Hum. Genet. 112, 204-208.
4. Farina, A., LeShane, E. S., Lambert-Messerlian, G. M., Canick, J. A., Lee, T., and Neveux, L. M. (2003) Valuation of fetal cell free DNA as a second-trimester marker of Down syndrome pregnancy. Clin. Chem. 49, 239-242.
5. Bianchi, D. W., LeShane, E., and Cowan, J. M. (2001) Large amounts of cell-free fetal DNA are present in amniotic fluid. Clin. Chem. 47, 1867-1869.
6. Larrabee, P. B., Johnson, K. L., Pestova, E., et al. (2004) Microarray analysis of cell-free fetal DNA in amniotic fluid: a prenatal molecular karyotype. Am. J. Hum. Genet. 75, 485-491.
7. Lapaire, O., Stroh, H., Peter, I., et al. (2006) Larger filters and change of lysis buffer significantly improve the quantity of extracted cell-free DNA from amniotic fluid. Clin. Chem. 52, 156-157.