Drug-to-antibody ratio (DAR) by UV/Vis spectroscopy

Methods in Molecular Biology

Volumn 1045, Issue , 2013, Pages 267-273

  Chen, Yan ^a  

^a GENENTECH INC   (United States)

Author keywords

Antibody drug conjugate; Drug to antibody ratio; Extinction coefficient; UV Vis

Indexed keywords

ANTINEOPLASTIC AGENT; CALICHEAMICIN; DOXORUBICIN; DRUG ANTIBODY; MAYTANSINOID DM; METHOTREXATE; RACHELMYCIN; TRASTUZUMAB; TRASTUZUMAB EMTANSINE; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; ARTICLE; DRUG ABSORPTION; DRUG DETERMINATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 84934436311     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-541-5_16     Document Type: Article

References (18)

1. Wakankar A, Chen Y, Gokarn Y (2011) Analytical methods for physicochemical characterization of antibody drug conjugates. MAbs 3:161-172
2. Hamblett KJ, Senter PD, Chace DF et al (2004) Effects of drug loading on the antitumor activity of a monoclonal antibody drug conjugate. Clin Cancer Res 10:7063-7070
3. Hinman LM, Hamann PR, Wallace R et al (1993) Preparation and characterization of monoclonal antibody conjugates of the calicheamicins: A novel and potent family of antitumor antibiotics. Cancer Res 53:3336-3342
4. Siegel MM, Hollander IJ, Hamann PR et al (1991) Matrix-Assisted UV-laser desorption/ ionization mass spectrometric analysis of monoclonal antibodies for the determination of carbohydrate, conjugated chelator and conjugated drug content. Anal Chem 63:2470-2481
5. Lazar AC, Wang L, Blattler WA et al (2005) Analysis of the composition of immunoconjugates using size-exclusion chromatography coupled to mass spectrometry. Rapid Commun Mass Spectrom 19:1806-1814
6. Schoonheydt RA (2010) UV-VIS-NIR spectroscopy and microscopy of heterogeneous catalysts. Chem Soc Rev 39:5051-5066
7. Pretsch E, Buhlmann P, Badertscher M (2009) Structure determination of organic compounds, tables of spectral data, 4th edn. Springer, New York, pp 401-420
8. Schmid F (2001) Biological macromolecules: UV-visible spectrophotometry. Encyclopedia Life sci. doi:10.1038/npg.els.0003142
9. Wang L, Amphlett G, Blattler WA et al (2005) Structural characterization of the maytansinoid-monoclonal antibody immunoconjugate, huN901-DM1, by mass spectrometry. Protein Sci 14:2436-2446
10. Hudecz F, Garnett MC, Khan T et al (1992) The influence of synthetic conditions on the stability of methotrexate-monoclonal antibody conjugates determined by reversed phase high performance liquid chromatography. Biomed Chromatogr 6:128-132
11. Chari RV, Jackel KA, Bourret LA et al (1995) Enhancement of the selectivity and antitumor efficacy of a CC-1065 analogue through immunoconjugate formation. Cancer Res 55:4079-4084
12. Greenfield RS, Kaneko T, Daues A et al (1990) Evaluation in vitro of adriamycin immunoconjugates synthesized using an acid-sensitive hydrazone linker. Cancer Res 50:6600-6607
13. Willner D, Trail PA, Hofstead SJ et al (1993) (6-Maleimidocaproyl) hydrazone of doxorubicin -A new derivative for the preparation of immunoconjugates of doxorubicin. Bioconjug Chem 4:521-527
14. Moran J (2002) Presentation at 224th American Chemical Society National Meeting, 18-22 Aug, Boston, MA
15. Laguzza BC, Nichols CL, Briggs SL et al (1989) New antitumor monoclonal antibodyvinca conjugates LY203725 and related compounds: Design, preparation and representative in vivo activity. J Med Chem 32:548-555
16. Benson AM, Suruda AJ, Talalay P (1975) Concentration-dependent association of A-3-ketosteroid isomerase of Pseudomonas testosteroni. J Biol Chem 250:276-280
17. Jaenicke L (1974) A rapid micromethod for the determination of nitrogen and phosphate in biological material. Anal Biochem 61:623-627
18. Pace CN, Vajdos F, Fee L et al (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci 4:2411-2423