Tandem metal-oxide affinity chromatography for enhanced depth of phosphoproteome analysis

Methods in Molecular Biology

Volumn 1072, Issue , 2014, Pages 621-632

  Beckers, Gerold J M ^a   Hoehenwarter, Wolfgang ^a   Röhrig, Horst ^a   Conrath, Uwe ^a   Weckwerth, Wolfram ^a  

^a UNIVERSITY OF VIENNA   (Austria)

Author keywords

LC MS; Orbitrap; Phosphoproteomics; Tandem MOAC

Indexed keywords

PHOSPHOPEPTIDE; PHOSPHOPROTEOME; PROTEOME; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CATION EXCHANGE; COLLISIONALLY ACTIVATED DISSOCIATION; ELECTRON TRANSPORT; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOSPHOPROTEOMICS; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; PROTEOMICS; SOLID PHASE EXTRACTION; TANDEM METAL OXIDE AFFINITY CHROMATOGRAPHY;

EUKARYOTA;

ALUMINUM OXIDE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; MASS SPECTROMETRY; PHOSPHOPROTEINS; PROTEOME; PROTEOMICS; TITANIUM;

EID: 84934435620     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-631-3_42     Document Type: Article

References (9)

1. Wolschin F, Weckwerth W (2005) Combining metal oxide affi nity chromatography (MOAC) and selective mass spectrometry for robust identifi cation of in vivo protein phosphorylation sites. Plant Methods 1:9
2. Wolschin F, Wienkoop S, Weckwerth W (2005) Enrichment of phosphorylated proteins and peptides from complex mixtures using metal oxide/hydroxide affi nity chromatography (MOAC). Proteomics 5:4389-4397
3. Hoehenwarter W, Thomas M, Nukarinen E et al (2013) Identifi cation of novel in vivo MAP kinase substrates in Arabidopsis thaliana through use of tandem metal oxide affi nity chromatography. Mol Cel Proteomics 12:369-380
4. Molina H, Horn DM, Tang N et al (2007) Global proteomic profi ling of phosphopeptides using electron transfer dissociation tandem mass spectrometry. Proc Natl Acad Sci USA 104:2199-2204
5. Hoehenwarter W, van Dongen JT, Wienkoop S et al (2008) A rapid approach for phenotypescreening and database independent detection of cSNP/protein polymorphism using mass accuracy precursor alignment. Proteomics 8:4214-4225
6. Egelhofer V, Wolfgang H, Lyon D et al (2013) Using ProtMAX to create high mass accuracy precursor alignments from label-free quantitative mass spectrometry data generated in large shotgun proteomics experiments. Nat Protoc 8(3):595-601
7. Chen Y, Hoehenwarter W, Weckwerth W (2010) Comparative analysis of phytohormoneresponsive phosphoproteins in Arabidopsis thaliana using TiO2-phosphopeptide enrich-ment and mass accuracy precursor alignment. Plant J 63:1-17
8. Sun X, Weckwerth W (2012) COVAIN: a toolbox for uni-and multivariate statistics, time-series and correlation network analysis and inverse estimation of the differential Jacobian from metabolomics covariance data. Metabolomics 8:81-93
9. Cox J, Matic I, Hilger M et al (2009) A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc 4:698-705