Purification and proteomic analysis of synaptic vesicles

Methods in Molecular Biology

Volumn 432, Issue , 2008, Pages 259-274

  Cox, Holly D ^a,b   Thompson, Charles M ^b  

^a UNIVERSITY OF MONTANA   (United States)

^b Department of Pathology ^*

Author keywords

16 BAC SDS PAGE; 2 D PAGE; Mass spectrometry; Membrane proteins; Synaptic vesicles

Indexed keywords

CAENORHABDITIS ELEGANS; MUS;

EID: 84934435010     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-28-7_18     Document Type: Article

References (8)

1. Vijayakrishnan, N. and Broadie, K. (2006) Temperature-sensitive paralytic mutants: insights into the synaptic vesicle cycle. Biochem. Soc. Trans. 34, 81-87.
2. Fernandez-Chacon, R. and Sudhof, T. C. (1999) Genetics of synaptic vesicle function: toward the complete functional anatomy of an organelle. Annu. Rev. Physiol. 61, 753-776.
3. Gualix, J., Pintor, J., and Miras-Portugal, M. T. (1999) Characterization of nucleotide transport into rat brain synaptic vesicles. J. Neurochem. 73, 1098-1104.
4. Goncalves, P. P., Meireles, S. M., Neves, P., and Vale, M. G. (2000) Distinction between Ca(2+) pump and Ca(2+)/H(+) antiport activities in synaptic vesicles of sheep brain cortex. Neurochem. Int. 37, 387-396.
5. Ikemoto, A., Bole, D. G., and Ueda, T. (2003) Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase. J. Biol. Chem. 278, 5929-5940.
6. Coughenour, H. D., Spaulding, R. S., and Thompson, C. M. (2004) The synaptic vesicle proteome: a comparative study in membrane protein identification. Proteomics 4, 3141-3155.
7. 7. Huttner, W. B., Schiebler, W., Greengard, P., and De Camilli, P. (1983) Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation. J. Cell Biol. 96, 1374-1388.
8. Hartinger, J., Stenius, K., Hogemann, D., and Jahn, R. (1996) 16-BAC/SDS-PAGE: a two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal. Biochem. 240, 126-133.