Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells: A "one plasmid" mammalian two-hybrid system

Methods in Molecular Biology

Volumn 911, Issue , 2012, Pages 175-182

  Tanaka, Tomoyuki ^a   Rabbitts, Terence H ^b  

^a ST JAMES S UNIVERSITY HOSPITAL   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Competition; IAC; iDab; Intrabodies; Intracellular antibodies; M2H; Mammalian two hybrid; Protein protein interaction; Single domains

Indexed keywords

ANTIBODY; DNA; INTRACELLULAR SINGLE DOMAIN ANTIBODY FRAGMENT; OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; DNA SEQUENCE; MAMMALIAN TWO HYBRID SYSTEM; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION;

EID: 84934434931     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-968-6_11     Document Type: Article

References (13)

1. Tanaka T, Lobato MN, Rabbitts TH (2003) Single domain intracellular antibodies: a minimal fragment for direct in vivo selection of antigen-speci fic intrabodies. J Mol Biol 331:1109-1120
2. Colby DW, Chu Y, Cassady JP, Duennwald M, Zazulak H, Webster JM, Messer A, Lindquist S, Ingram VM, Wittrup KD (2004) Potent inhibition of huntingtin aggregation and cytotoxic-ity by a disulfide bond-free single-domain intracellular antibody. Proc Natl Acad Sci USA 101:17616-17621
3. Tanaka T, Sewell H, Waters S, Philips S, Rabbitts TH (2011) Single domain intracellu-lar antibodies from diverse libraries: emphasizing dual functions of LMO2 protein interactions using a VH single domain. J Biol Chem 286:3707-3716
4. Tse E, Lobato MN, Forster A, Tanaka T, Chung GTY, Rabbitts TH (2002) Intracellular antibody capture technology: application to selection of single chain Fv recognising the BCR-ABL oncogenic protein. J Mol Biol 317:85-94
5. Tanaka T, Rabbitts TH (2010) Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture. Nat Protoc 5:67-92
6. Tanaka T, Williams RL, Rabbitts TH (2007) Tumour prevention by a single antibody domain inhibiting binding of signal transduc-tion molecules to activated RAS. EMBO J 26:3250-3259
7. Bartel PL, Chien C, Sternglanz R, Fields S (1993) Using the two-hybrid system to detect protein-protein interaction. In: Hartley DA (ed) Cellular interaction in development: a practical approach. IRL Press, Oxford, pp 153-179
8. Visintin M, Tse E, Axelson H, Rabbitts TH, Cattaneo A (1999) Selection of antibodies for intracellular function using a two-hybrid in vivo system. Proc Natl Acad Sci USA 96:11723-11728
9. Vojtek AB, Hollenberg SM, Cooper JA (1993) Mammalian Ras interacts directly with the ser-ine/threonine kinase Raf. Cell 74:205-214
10. Sambrook J, Russell PW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
11. Engebrecht JBR, Kaderbhai MA (1992) Escherichia coli, plasmids, and bacteriophages. Wiley, New York
12. Sadowski I, Bell B, Broad P, Hollis M (1992) GAL4 fusion vectors for expression in yeast or mammalian cells. Gene 118:137-141
13. Tucker KL, Beard C, Dausmann J, Jackson-Grusby L, Laird PW, Lei H, Li E, Jaenisch R (1996) Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev 10:1008-1020