Plasma membrane isolation using immobilized concanavalin a magnetic beads

Methods in Molecular Biology

Volumn 909, Issue , 2012, Pages 29-41

  Lee, Yu Chen ^a   Gajdosik, Martina Srajer ^b,c   Josic, Djuro ^c,d   Lin, Sue Hwa ^a  

^a UNIVERSITY OF TEXAS   (United States)

^b JOSIP JURAJ STROSSMAYER UNIVERSITY OF OSIJEK   (Croatia)

^c BROWN UNIVERSITY   (United States)

^d University of Rijeka   (Croatia)

Author keywords

Concanavalin A; Liver plasma membrane; Magnetic beads

Indexed keywords

CONCANAVALIN A; LIVER PROTEIN; NANOBEAD; PROTEOME;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CELL ISOLATION; ELECTROSPRAY MASS SPECTROMETRY; GLYCOSYLATION; LIQUID CHROMATOGRAPHY; LIVER CELL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 84934434699     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-959-4_3     Document Type: Article

References (9)

1. Boone CW, Ford LE, Bond HE, Stuart DC, Lorenz D (1969) Isolation of plasma membrane fragments from HeLa cells. J Cell Biol 41:378-392
2. Lawson EL, Clifton JG, Huang F, Li X, Hixson DC, Josic D (2006) Use of magnetic beads with immobilized monoclonal antibodies for isolation of highly pure plasma membranes. Electrophoresis 27:2747-2758
3. Bhattacharyya L, Ceccarini C, Lorenzoni P, Brewer CF (1987) Concanavalin A interactions with asparagine-linked glycopeptides. Bivalency of high mannose and bisected hybrid type glycopeptides. J Biol Chem 262: 1288-1293
4. Neville DMJ (1968) Isolation of an organ speci fic protein antigen from cell surface membrane of rat liver. Biochim Biophys Acta 154:540-552
5. Cheung PH, Luo W, Qiu Y, Zhang X, Earley K, Millirons P et al (1993) Structure and function of C-CAM1. The first immunoglobulin domain is required for intercellular adhesion. J Biol Chem 268:24303-24310
6. Clifton J, Huang F, Rucevic M, Cao L, Hixson D, Josic Dj (2011) Protease inhibitors as possible pitfalls in proteomic analyses of complex biological samples. J Proteomics 74: 935-941
7. Eng JK, McCormack AL, Yates JR III (1994) An approach to correlate tandem mass spectral data of peptides with amino acid sequence in a protein database. J Am Soc Mass Spectrom 5:976-989
8. Elias JE, Gygi SP (2007) Target-decoy search strategy for increased con fidence in large-scale protein identi fication by mass spectrometry. Nat Methods 4:207-214
9. Lee YC, Block G, Chen H, Folch-Puy E, Foronjy R, Jalili R et al (2008) One-step isolation of plasma membrane proteins using magnetic beads with immobilized concanavalin A. Protein Expr Purif 62:223-229