PCR and RFLP analyses based on the ribosomal protein operon

Methods in Molecular Biology

Volumn 938, Issue , 2013, Pages 173-188

  Martini, Marta ^a   Lee, Ing Ming ^b  

^a UNIVERSITY OF UDINE   (Italy)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

16Sr group speci fi c primers; 16SR subgroup specific primers; Phytoplasma strain differentiation; Restriction enzymes; RplV ( rpl22 ); Rpsc ( rps3 ); Semiuniversal primers

Indexed keywords

ACRYLAMIDE; DNA; N,N' METHYLENEBISACRYLAMIDE; POLYACRYLAMIDE GEL; REAGENT; RESTRICTION ENDONUCLEASE; RIBOSOME PROTEIN; RNA 16S;

AGAR GEL ELECTROPHORESIS; ARTICLE; GENE SEQUENCE; NONHUMAN; OPERON; PHYTOPLASMA; PLANT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RIBOSOME; STRAIN DIFFERENCE;

EID: 84934434631     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-89-2_15     Document Type: Article

References (16)

1. Zhao Y et al (2010) Recent advances in 16S rRNA gene-based phytoplasma differentiation, classification and taxonomy. In: Weintraub PG, Jones P (eds) Phytoplasmas: genomes, plant hosts and vectors. CAB International, Wallingford, pp 64-92
2. Lee I-M et al (2011) ' Candidatus Phytoplasma costaricanum' a new phytoplasma associated with a newly emerging disease in soybean in Costa Rica. Int J Syst Evol Microbiol. doi: 10.1099/ijs.0.029041-0
3. Lee I-M, Zhao Y, Davis RE (2010) Prospects of multiple gene-based systems for differentiation and classification of phytoplasmas. In: Weintraub PG, Jones P (eds) Phytoplasmas: genomes, plant hosts and vectors. CAB International, Wallingford, pp 51-63
4. Lee I-M et al (1998) Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153-1169
5. Lee I-M et al (2004) ' Candidatus Phytoplasma asteris', a novel phytoplasma taxon associated with aster yellows and related diseases. Int J Syst Evol Microbiol 54:1037-1048
6. Lee I-M et al (2004) Classification of phytoplasma strains in the elm yellows group (16SrV) and proposition of ' Candidatus Phytoplasma ulmi' for the phytoplasma associated with elm yellows. Int J Syst Evol Microbiol 54:337-347
7. Martini M et al (2002) Genetic variability among fl avescence dorée phytoplasmas from different origins in Italy and France. Mol Cell Probes 16:197-208
8. Martini M et al (2007) Ribosomal protein gene-based phylogeny for fi ner differentiation and classification of phytoplasmas. Int J Syst Evol Microbiol 57:2037-2051
9. Lim P-O, Sears BB (1992) Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences. J Bacteriol 174:2606-2611
10. Lee I-M et al (2003) Ecological implications from a molecular analysis of phytoplasmas involved in an aster yellows epidemic in various crops in Texas. Phytopathology 93:1368-1377
11. Martini M (2004) Ribosomal protein genebased phylogeny: a basis for phytoplasma classification. PhD dissertation, University of Udine, Udine, Italy, p 106
12. Martini M et al (2008) Molecular differentiation of ' Candidatus Phytoplasma mali' and its spreading in Friuli Venezia Giulia region (north-east Italy). Acta Hortic 781:395-402
13. Gundersen DE et al (1996) Genomic diversity and differentiation among phytoplasma strains in 16S rRNA group I (aster yellows and related phytoplasmas) and III (X-disease and related phytoplasmas). Int J Syst Bacteriol 46:64-75
14. Grif fi ths HM et al (1999) The phytoplasma associated with ash yellows and lilac witches'- broom: ' Candidatus Phytoplasma fraxini'. Int J Syst Bacteriol 49:1605-1614
15. Durante G et al (2008) Bois noir in Lombardy (northern Italy): Identification of molecular markers for diagnosis and characterization of 16SrXII-A phytoplasmas. Proceedings of the: 4th National Workshop on Phytoplasma Diseases. Rome, Italy 28-30 May 2008, p 300-303
16. Streten C, Gibb KS (2005) Genetic variation in Candidatus Phytoplasma australiense. Plant Pathol 54:8-14