Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens

Methods in Molecular Biology

Volumn 493, Issue , 2009, Pages 179-190

  Thomas, Jonathan C ^a   Pettigrew, Melinda M ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

eBURST; Haemophilus influenzae; Molecular epidemiology; Moraxella catarrhalis; Multilocus sequence typing; Otitis media; Pulsed field gel electrophoresis; Streptococcus pneumoniae

Indexed keywords

BACTERIA (MICROORGANISMS); HAEMOPHILUS INFLUENZAE; MORAXELLA CATARRHALIS; STREPTOCOCCUS PNEUMONIAE;

EID: 84934434621     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-523-7_11     Document Type: Article

References (14)

1. Maiden, M. C., Bygraves, J. A., Feil, E., Morelli, G., Russell, J. E., Urwin, R., Zhang, Q., Zhou, J., Zurth, K., Caugant, D. A., Feavers, I. M., Achtman, M. and Spratt, B. G. (1998) Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. U.S.A. 95, 3140-3145.
2. Enright, M. and Spratt, B. (1998) A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144, 3049-3060.
3. Meats, E., Feil, E. J., Stringer, S., Cody, A. J., Goldstein, R., Kroll, J. S., Popovic, T. and Spratt, B. G. (2003) Characterization of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. J. Clin. Microbiol. 41, 1623-1636.
4. Feil, E. J. and Enright, M. C. (2004) Analyses of clonality and the evolution of bacterial pathogens. Curr. Opinion Microbiol. 7, 308-313.
5. Lee, M., Lee, Y. and Chiou, C. (2006) The suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Bordetella pertussis. Diagn. Microbiol. Infect. Dis. 56, 217-219.
6. Schwartz, D. C. and Cantor, C. R. (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37, 67-75.
7. Birren, B. and Lai, E. (1994) Rapid pulsed field separation of DNA molecules up to 250 kb. Nucleic Acids Res. 22, 5366-5370.
8. Dagan, R., Leibovitz, G., Cheletz, G., Leiberman, A. and Porat, N. (2001) Antibiotic treatment in acute otitis media promotes superinfection with resistant Streptococcus pneumoniae carried before initiation of treatment. J. Infect. Dis. 183, 800-806.
9. Yano, H., Suetake, M., Kuga, A., Irinoda, K., Okamoto, R., Kobayashi, T. and Inoue, M. (2000) Pulsed-field gel electrophoresis analysis of nasopharyngeal flora in children attending a day care center. J. Clin. Microbiol. 38, 625-629.
10. Pettigrew, M. M., Foxman, B., Ecevit, Z., Marrs, C. F. and Gilsdorf, J. (2002) Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae. J. Clin. Microbiol. 40, 660-662.
11. Malchowa, N., Sabat, A., Gniadkowski, M., Krzyszton-Russjan, J., Empel, J., Miedzobrodzki, J., Kosowska-Shick, K., Appelbaum, P. C. and Hryniewicz, W. (2005) Comparison of multiple-locus variable-number tandem repeat analysis with pulsed-field gel electophoresis, spa typing, and multilocus sequence typing for clonal characterization of Staphylococcus aureus isolates. J. Clin. Microbiol. 43, 3095-3100.
12. Pettigrew, M. M., Fennie, K. P., York, M. P., Daniels, J. and Ghaffar, F. (2006) Variation in the presence of neuraminidase genes among Streptococcus pneumoniae isolates with identical sequence types. Infect. Immun. 74, 3360-3365.
13. Feil, E. J., Li, B.C., Aanensen, D. M., Hanage, W. P. and Spratt, B. G. (2004) eBURST: Inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J. Bacteriol. 186, 1518-1530.
14. Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H. and Swaminathan, B. (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33, 2233-2239.